11 research outputs found
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms
DNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. In Candida albicans, there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. This work examined the presence and quantified the amount of eDNA in C. albicans biofilm ECM and the effect of DNase treatment and the addition of exogenous DNA on C. albicans biofilm development as indicators of a role for eDNA in biofilm development. We were able to detect the accumulation of eDNA in biofilm ECM extracted from C. albicans biofilms formed under conditions of flow, although the quantity of eDNA detected differed according to growth conditions, in particular with regards to the medium used to grow the biofilms. Experiments with C. albicans biofilms formed statically using a microtiter plate model indicated that the addition of exogenous DNA (>160 ng/ml) increases biofilm biomass and, conversely, DNase treatment (>0.03 mg/ml) decreases biofilm biomass at later time points of biofilm development. We present evidence for the role of eDNA in C. albicans biofilm structure and formation, consistent with eDNA being a key element of the ECM in mature C. albicans biofilms and playing a predominant role in biofilm structural integrity and maintenance.National Institute of Dental & Craniofacial ResearchFundação para a Ciência e
Tecnologia (FCT) - SFRH/BD/28222/2006National Institute of Allergy and
Infectious Disease
Genetic variants and their interactions in disease risk prediction – machine learning and network perspectives
Regulation of protein degradation pathways by amino acids and insulin in skeletal muscle of neonatal pigs
Oxytocin biases eye-gaze to dynamic and static social images and the eyes of fearful faces: associations with trait autism
Anterior knee pain and functional outcome following different surgical techniques for tibial nailing: a systematic review
Yeast Biofilms
Yeast biofilms are an escalating clinical problem, which affect both the healthy and immunocompromised, and are related to significant rates of mortality within hospitalized patients. Candida albicans is the most notorious yeast biofilm former and as a result the most widely studied; however, other Candida species and yeasts such as Cryptococcus neoformans are also implicated in biofilm-associated infections. Yeast biofilms have distinct developmental phases, including adhesion, colonization, maturation and dispersal, which have been examined utilizing various in vitro and in vivo model systems. Furthermore, the complex molecular events governing biofilm development are slowly being elucidated, including the role of quorum sensing. Clinically, biofilms act as reservoirs for systemic infection, and also induce localized pathology and tissue damage. However, the key virulence factor is their recalcitrance to antifungal therapy. This chapter will discuss our current understanding of the role that yeast biofilms play in the clinical setting