Fertirrigação nitrogenada para produção de macieiras Eva e Princesa em Petrolina-PE.

O presente trabalho foi desenvolvido com o objetivo de avaliar produção de frutos das 26 macieiras ?Eva? e ?Princesa?, em função a fertirrigação nitrogenada em Petrolina-PE

Qualidade de frutos de macieiras Eva e Princesa sob fertirrigação nitrogenada em Petrolina-PE.

O presente trabalho foi desenvolvido com o objetivo de avaliar a qualidade física e química de frutos das macieiras Eva e Princesa, em função a fertirrigação nitrogenada em Petrolina-PE

Clonagem do abacateiro variedade "Duke 7" (Persea americana Mill.) por alporquia.

O objetivo deste trabalho foi avaliar o efeito do anelamento e do ácido indol-butirico (AIB), em mudas de abacateiro da variedade ?Duke 7?, com a finalidade de se obter material clonal que possa ser utilizado como porta-enxerto para as variedades tradicionalmente cultivados

Influência da época do ano no sucesso da enxertia nas variedades de abacateiro: Hass e Fortuna.

O objetivo deste trabalho foi identificar a época do ano na qual ocorre o melhor pegamento do enxerto de abacate, em duas variedades: Hass e Fortuna, durante o período de doze meses

Impact of fulvic acid and free amino acids on paclobutrazol absorption by Keitt mango.

This study evaluated the impact of fulvic acid and free amino acids on paclobutrazol soil residue, their absorption and effects on ?Keitt? mango grown in tropical semi-arid environmental conditions. The experiment was carried out from 2017 to 2018 simultaneously in two orchards with the same plants and management characteristics, located in Cabrobó, Pernambuco, Brazil. The experimental design was randomized blocks with four treatments, five replications and four plants per replication. The treatments consisted of paclobutrazol combinations with acid fulvic and free amino acids, as follows: Treatment 1: paclobutrazol + water (control); Treatment 2: paclobutrazol + fulvic acids; Treatment 3: paclobutrazol + free amino acids; and Treatment 4: paclobutrazol + fulvic acids + free amino acids. According to the results, the use of fulvic acids, free amino acids or both affects the paclobutrazol absorption by 'Keitt' mango. The addition of fulvic acid to the paclobutrazol improves the absorption of this molecule by the plant, with greater inhibition of vegetative growth of 'Keitt' mango and lower soil residues

Structural and functional evidence for membrane docking and disruption sites on phospholipase A2-like proteins revealed by complexation with the inhibitor suramin

Local myonecrosis resulting from snakebite envenomation is not efficiently neutralized by regular antivenom administration. This limitation is considered to be a significant health problem by the World Health Organization. Phospholipase A2-like (PLA2-like) proteins are among the most important proteins related to the muscle damage resulting from several snake venoms. However, despite their conserved tertiary structure compared with PLA2s, their biological mechanism remains incompletely understood. Different oligomeric conformations and binding sites have been identified or proposed, leading to contradictory data in the literature. In the last few years, a comprehensive hypothesis has been proposed based on fatty-acid binding, allosteric changes and the presence of two different interaction sites. In the present study, a combination of techniques were used to fully understand the structural-functional characteristics of the interaction between suramin and MjTX-II (a PLA2-like toxin). In vitro neuromuscular studies were performed to characterize the biological effects of the protein-ligand interaction and demonstrated that suramin neutralizes the myotoxic activity of MjTX-II. The high-resolution structure of the complex identified the toxin-ligand interaction sites. Calorimetric assays showed two different binding events between the protein and the inhibitor. It is demonstrated for the first time that the inhibitor binds to the surface of the toxin, obstructing the sites involved in membrane docking and disruption according to the proposed myotoxic mechanism. Furthermore, higher-order oligomeric formation by interaction with interfacial suramins was observed, which may also aid the inhibitory process. These results further substantiate the current myotoxic mechanism and shed light on the search for efficient inhibitors of the local myonecrosis phenomenon.Peer Reviewe