15 research outputs found
Effect of restricted feeding and realimentation on feed performance and carcass characteristics of growing lambs
Residual feed intake and relationships with performance of Nellore cattle selected for post weaning weight
Desempenho e características quantitativas de carcaça de novilhos Braford desmamados aos 100 ou 180 dias de idade e abatidos aos 13-14 meses
Efeito da idade de desmame no desenvolvimento e nas características de carcaça de novilhos de corte
Consumo alimentar residual e sua relação com medidas de desempenho e eficiência e características in vivo da carcaça de cordeiros
Características das partes do corpo não-integrantes da carcaça de novilhos jovens e superjovens de diferentes grupos genéticos
Conteúdo corporal e exigências líquidas de energia e proteína de novilhos mestiços Holandês-Gir em ganho compensatório
Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length
Willow (Salix L.) species have good qualities for becoming a biomass crop for production of biofuels. They grow on marginal soils, produce high yields of wood in a short period of time, and are easily propagated. Salix is one of the few genera that may be cryopreserved as dormant winter buds (DBs) instead of using tissue culture techniques. The objective of our study was to evaluate selected factors that affect cryotolerance of nine Salix germplasm accessions in the USDA‐ARS National Plant Germplasm System collection. One‐year‐old branches harvested in January over 3 yr were cut into either 6‐ to 7‐ or 10‐cm segments containing at least two DBs. Segments were treated with a slow‐cooling procedure and stored in vapor‐phase liquid N (LNV). Control segments were stored at −5 °C until planting. The LNV‐exposed and the −5 °C‐stored nodal segments were warmed and directly planted in a cold greenhouse. Six weeks later, the material was evaluated for shoot and root development. Segments that developed a shoot were considered viable. Average viability varied among years (0–35.1%) even for the same accession, species (4.2–51.4%), and segment length. Dormant buds on 10‐cm segments had higher average viability (82.2%) than did DBs on 6‐ to 7‐cm segments (43.9%), suggesting higher suitability for cryopreservation. In the material studied, Salix DB viability was correlated with branch diameter, DB density, and the ability to develop shoots and roots prior to cryopreservation