Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 Emend. Levine, 1961 in experimentally infected goat.

Abstract: The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the surface of multinucleated meronts. Free merozoites in the parasitophorous vacuole present a conoid, double membrane, one pair of rhoptries, micronemes, micropore, anterior and posterior polar ring, a nucleus with a nucleolus and peripheral chromatin. The microgamonts are located below the nucleus of the host cell and contain several nuclei at the periphery of the parasite. The microgametes consist of a body, a nucleus, three flagella and mitochondria. The macrogamonts develop below the nucleus of the host cell and have a large nucleus with a prominent nucleolus. The macrogametes contain a nucleus, wall-forming bodies of type I and type II. The young oocysts present a wall containing two layers and a sporont

Perfil dos produtores rurais do Rio Pardo de Minas: estudo de caso nas comunidades Monte Alegre, Vereda Funda e Água Boa (I e II).

Resumo - O estudo objetiva traçar o perfil técnico-econômico, social, ambiental e institucional dos produtores rurais de três comunidades do município de Rio Pardo de Minas (Vereda Funda; Água Boa I e II e Monte Alegre), sendo um esforço inicial na busca por compreender as necessidades, demandas e aspirações da agricultura familiar local. O levantamento é resultado de dados primários coletados por meio de visitas exploratórias e da aplicação de 92 questionários semiestruturados. Um resultado interessante foi perceber que os produtores rurais, embora com baixos níveis de produtividade, escolaridade e de capacitação técnica, têm consciência da necessidade da preservação ambiental, dos seus fatores críticos e dos gargalos estruturais que determinam o fraco nível de desenvolvimento econômico local. O estudo permitiu concluir que algumas soluções tecnológicas simples e intervenções públicas plausíveis poderiam gerar benefícios imediatos para as comunidades, tais como: melhorar os sistemas de produção e práticas de manejo; aumentar a qualidade da mão de obra por meio de capacitação e assistência técnica; direcionar mais crédito rural para investimento em cisternas, poços artesianos, sistemas de irrigação, insumos agropecuários (como máquinas e equipamentos, adubos e fertilizantes, defensivos agrícolas), dentre outros. Abstract - The study aims to trace the technical, economic, social, environmental and institutional profile of farmers in three communities of Rio Pardo de Minas (Vereda Funda, Água Boa I and II and Monte Alegre), being an initial effort in the search for understanding the needs, demands and aspirations of the local family farmers. The survey is the result of primary data collected through exploratory visits and application of 92 semi-structured questionnaires. An interesting result was to realize that farmers, albeit with low levels of productivity, education and technical training, are very aware of the environmental preservation´s need and the critical structural factors that determine the low level of local economic development. The study concluded that some simple technological solutions and plausible public interventions could bring immediate benefits to the three communities farmers, such as improving production systems and management practices; increase the quality of labor force through training and technical assistance; direct more rural credit for investment in water tanks, wells, irrigation systems, agricultural inputs (such as machinery and equipment, fertilizers, pesticides), among others

Perfil dos produtores rurais de Rio Pardo de Minas: estudo de caso nas comunidades Monte Alegre, Vereda Funda e Água Boa (I e II).

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Non-typeable Haemophilus influenzae and Streptococcus pneumoniae as primary causes of acute otitis media in colombian children: a prospective study

<p>Abstract</p> <p>Background</p> <p>Acute otitis media (AOM) is one of the most frequently encountered bacterial infections in children aged < 5 years; <it>Streptococcus pneumoniae </it>(<it>S. pneumoniae</it>) and non-typeable <it>Haemophilus influenzae </it>(NTHi) are historically identified as primary AOM causes. Nevertheless, recent data on bacterial pathogens causing AOM in Latin America are limited. This prospective study aimed to identify and characterize bacterial etiology and serotypes of AOM cases including antimicrobial susceptibility in < 5 year old Colombian children.</p> <p>Methods</p> <p>From February 2008 to January 2009, children ≥3 months and < 5 years of age presenting with AOM and for whom a middle ear fluid (MEF) sample was available were enrolled in two medical centers in Cali, Colombia. MEF samples were collected either by tympanocentesis procedure or spontaneous otorrhea swab sampling. Bacteria were identified using standard laboratory methods, and antimicrobial resistance testing was performed based on the 2009 Clinical and Laboratory Standards Institute (CLSI) criteria. Most of the cases included in the study were sporadic in nature.</p> <p>Results</p> <p>Of the 106 enrolled children, 99 were included in the analysis. Bacteria were cultured from 62/99 (63%) of samples with <it>S. pneumoniae, H. influenzae, or S. pyogenes</it>. The most commonly isolated bacteria were <it>H. influenzae </it>in 31/99 (31%) and <it>S. pneumoniae </it>in 30/99 (30%) of samples. The majority of <it>H. influenzae </it>episodes were NTHi (27/31; 87%). 19F was the most frequently isolated pneumococcal serotype (10/30; 33%). Of the 30 <it>S. pneumoniae </it>positive samples, 8/30 (27%) were resistant to tetracycline, 5/30 (17%) to erythromycin and 8/30 (27%) had intermediate resistance to penicillin. All <it>H. influenzae </it>isolates tested were negative to beta-lactamase.</p> <p>Conclusions</p> <p>NTHi and <it>S. pneumoniae </it>are the leading causes of AOM in Colombian children. A pneumococcal conjugate vaccine that prevents both pathogens could be useful in maximizing protection against AOM.</p

Differentiation of COVID-19 signs and symptoms from allergic rhinitis and common cold: An ARIA-EAACI-GA2LEN consensus

Background: Although there are many asymptomatic patients, one of the problems of COVID-19 is early recognition of the disease. COVID-19 symptoms are polymorphic and may include upper respiratory symptoms. However, COVID-19 symptoms may be mistaken with the common cold or allergic rhinitis. An ARIA-EAACI study group attempted to differentiate upper respiratory symptoms between the three diseases. Methods: A modified Delphi process was used. The ARIA members who were seeing COVID-19 patients were asked to fill in a questionnaire on the upper airway symptoms of COVID-19, common cold and allergic rhinitis. Results: Among the 192 ARIA members who were invited to respond to the questionnaire, 89 responded and 87 questionnaires were analysed. The consensus was then reported. A two-way ANOVA revealed significant differences in the symptom intensity between the three diseases (p&nbsp;&lt;.001). Conclusions: This modified Delphi approach enabled the differentiation of upper respiratory symptoms between COVID-19, the common cold and allergic rhinitis. An electronic algorithm will be devised using the questionnaire