PAISES BAJOS. Costa. Cartas náuticas. 1799. 1:580000

Carta arrumbada que comprende la costa de los Países Bajos, la de Bélgica, una pequeña parte de la noroccidental de Francia (hata Boulogne) y la suroriental de InglaterraFirmado por el autorEscalas gráficas de 60 millas inglesas y francesas de 60 al grado y de 15 millas alemanas y danesas de 15 al grado [= 19,3 cm]. Orientado con flecha en rosa de treinta y dos vientos en la que se señala además la variación magnética observadaIndica sondas batimétricas, veriles, bancos, bajos, fondeaderos, enfilaciones y dirección de las corrientes ; así como los faros existentes, destacados en rojo con posterioridd a la elaboración del documentoNota explicativa referente a los distintos tipos de boyas que regulan la navegaciónInserta : "A Chart of the Entrances to the Texel and Vlie / by W. Heather". Escala [ca, 1;310000], 15 millas inglesas [= 8,9 cm]Está incluido en un grupo de mapas dispuestos a modo de cuaderno : "North Sea" ; "The entrances to the River Thames" ; "East Coast of England" ; "East Coast of England and Scotland" ; "A New Chart of Holland with the Entraces to the Scheld" ; "A New and Improved chart of the Rivers Elbe and Weser to Hamburg and Bremen"Forma parte de la Colección Mendoz

INGLATERRA (Reino Unido) (Costa oriental). Cartas náuticas (1802). 1:270000

Comprende la costa oriental de Inglaterra desde el cabo Psurn hasta Orford NessLugar de publicación y editor tomados de los mapas que acompañas a ésteEscalas gráficas de 30 millas inglesas y francesas de 60 al grado, 8 millas suecas de 10 al grado y 7 1/2 millas danesas y alemanas de 15 al grado [= 20,5 cm]. Orientado con lis en rosa de treinta y dos vientos, en la que se señala además la variación magnética observadaIndica sondas batimétricas, veriles, bajos, bancos, fondeaderos, dirección de las corrientes, derrotas y enfilaciones ; así como los faros existentes, destacados en rojo con posterioridad a la publicación del documentoEstá incluido en un grupo de mapas dispuestos a modo de cuaderno : "North Sea" ; "The entrances to the River Thames" ; "East coast of England and Scotland" ; "A New Chart of Holland with the Entrances to the Scheld" ; "The Coasts of Holland and England" ; "A New and Improved Chart of the rivers Elbe and Weser to Hamburg and Bremen"Forma parte de la Colección Mendoz

VarSite: disease variants and protein structure

VarSite is a web server mapping known disease-associated variants from UniProt and ClinVar, together with natural variants from gnomAD, onto protein 3D structures in the Protein Data Bank (PDB). The analyses are primarily image-based and provide both an overview for each human protein, as well as a report for any specific variant of interest. The information can be useful in assessing whether a given variant might be pathogenic or benign. The structural annotations for each position in the protein include protein secondary structure, interactions with ligand, metal, DNA/RNA, or other protein, and various measures of a given variant's possible impact on the protein's function. The 3D locations of the disease-associated variants can be viewed interactively via the 3dmol.js JavaScript viewer, as well as in RasMol and PyMOL. Users can search for specific variants, or sets of variants, by providing the DNA coordinates of the base change(s) of interest. Additionally, various agglomerative analyses are given, such as the mapping of disease and natural variants onto specific Pfam or CATH domains. The server is freely accessible to all at: https://www.ebi.ac.uk/thornton-srv/databases/VarSite. This article is protected by copyright. All rights reserved

Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR

Introduction The ability to screen blood of early stage operable breast cancer patients for circulating tumour cells is of potential importance for identifying patients at risk of developing distant relapse. We present the results of a study of the efficacy of the immunobead RT-PCR method in identifying patients with circulating tumour cells. Results Immunomagnetic enrichment of circulating tumour cells followed by RT-PCR (immunobead RT-PCR) with a panel of five epithelial specific markers (ELF3, EPHB4, EGFR, MGB1 and TACSTD1) was used to screen for circulating tumour cells in the peripheral blood of 56 breast cancer patients. Twenty patients were positive for two or more RT-PCR markers, including seven patients who were node negative by conventional techniques. Significant increases in the frequency of marker positivity was seen in lymph node positive patients, in patients with high grade tumours and in patients with lymphovascular invasion. A strong trend towards improved disease free survival was seen for marker negative patients although it did not reach significance (p = 0.08). Conclusion Multi-marker immunobead RT-PCR analysis of peripheral blood is a robust assay that is capable of detecting circulating tumour cells in early stage breast cancer patients

REPUBLICA FEDERAL ALEMANA. Occidental. Costa. Cartas náuticas. 1795. 1:310000

Comprende la costa de la República Federal Alemana desde Emden a Husum, con el río Weser hasta Bremen y el Elba hasta HamburgoFigura la firma del autorEscalas gráficas de 30 millas inglesas de 60 al grado y 7 1/2 millas danesas y alemanas de 15 al grado [= 18,2 cm]. Orientado con lis en rosa de treinta y dos vientos, en la que se señala además la variación magnética observadaOrografía de perfilIndica sondas batimétricas, fondeaderos, bajos, enfilaciones y dirección de las corrientes ; así como los faros existentes, destacadas en rojo con posterioridad a la elaboración del documentoInserta : "A Particular Chart of Helgoland, from the late Survey / by J.T. Reinke and J.A. Land". Escala [ca. 1:33000], 1 milla inglesa [= 5,6 cm] ; "A View of Helegoland bearing N.N.W. 1/2 N. distant 3 Englis Miles" ; "A View of Helegoland bearing East distant 3 Miles" ; "A View of Helegoland bearing S. by E. distant 4 Miles"Está incluido en un grupo de mapas dispuestos a modo de cuaderno : "North Sea" ; "The entrances to the River Thames" ; "East Coast of England" ; "East Coast of England and Scotland" ; "A New Chart of Holland with the Entrances to the Scheld" ; "The Coasts of Holland and England"Forma parte de la Colección Mendoz

Coupling marine ecosystem state with environmental management and conservation: A risk-based approach

\ua9 2024 The Author(s)The sustainability of marine ecosystems demands a focus on ecological improvement, necessitating managers and conservationists to consider a range of actions from those that limit stressors to those that actively restore. Deciding the most appropriate action should be informed by environmental context, which includes assessing information on both degradation and recovery potential. Here, we provide an analysis of how the degree of ecological degradation coupled with the stressor regime can inform environmental management and conservation actions (e.g., stressor reductions, adaptive management, assisted recovery/restoration). With this analysis we design a risk framework combining principles that define ecosystem resilience and recovery times with those that characterize stressor regimes (i.e., the number, type, and impact). The combination of these principles defines where an ecosystem is placed along sliding scales of degradation and recovery and likely response to protective and restorative interventions. It is designed to facilitate place-based conversations regarding the risks of different management actions informed by the temporal dynamics of ecosystem degradation and recovery

Understanding pregnancy planning in a low-income country setting: validation of the London measure of unplanned pregnancy in Malawi

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: The London Measure of Unplanned Pregnancy (LMUP) is a new and psychometrically valid measure of pregnancy intention that was developed in the United Kingdom. An improved understanding of pregnancy intention in low-income countries, where unintended pregnancies are common and maternal and neonatal deaths are high, is necessary to inform policies to address the unmet need for family planning. To this end this research aimed to validate the LMUP for use in the Chichewa language in Malawi.Methods: Three Chichewa speakers translated the LMUP and one translation was agreed which was back-translated and pre-tested on five pregnant women using cognitive interviews. The measure was field tested with pregnant women who were recruited at antenatal clinics and data were analysed using classical test theory and hypothesis testing.Results: 125 women aged 15-43 (median 23), with parities of 1-8 (median 2) completed the Chichewa LMUP. There were no missing data. The full range of LMUP scores was captured. In terms of reliability, the scale was internally consistent (Cronbach's alpha = 0.78) and test-retest data from 70 women showed good stability (weighted Kappa 0.80). In terms of validity, hypothesis testing confirmed that unmarried women (p = 0.003), women who had four or more children alive (p = 0.0051) and women who were below 20 or over 29 (p = 0.0115) were all more likely to have unintended pregnancies. Principal component analysis showed that five of the six items loaded onto one factor, with a further item borderline. A sensitivity analysis to assess the effect of the removal of the weakest item of the scale showed slightly improved performance but as the LMUP was not significantly adversely affected by its inclusion we recommend retaining the six-item score.Conclusion: The Chichewa LMUP is a valid and reliable measure of pregnancy intention in Malawi and can now be used in research and/or surveillance. This is the first validation of this tool in a low-income country, helping to demonstrate that the concept of pregnancy planning is applicable in such a setting. Use of the Chichewa LMUP can enhance our understanding of pregnancy intention in Malawi, giving insight into the family planning services that are required to better meet women's needs and save lives. © 2013 Hall et al.; licensee BioMed Central Ltd.Dr Hall’s Wellcome Trust Research Training Fellowship, grant number 097268/Z/11/Z