Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii

This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids

Green Plant Biotechnology at work

The pages below contain the topics, questions, and instruction of a course on Plant Biotechnology and Molecular Plant Breeding, which is taken by students at the Universities of Applied Sciences in the Netherlands who specialize in Green Biotechnology. In the course they learn about the use of molecular techniques for applications in plant breeding companies and in related research in The Netherlands