13 research outputs found
Tobamovirus and Dianthovirus Movement Proteins Are Functionally Homologous
AbstractThe movement proteins (MPs) of tobacco mosaic tobamovirus (TMV) and red clover necrotic mosaic dianthovirus (RCNMV) enlarge plasmodesmata size exclusion limits, transport RNA from cell to cell, and bind nucleic acids in vitro. Despite these functional similarities, they have no sequence homology. However, they do appear to have similar secondary structures. We have used transgenic plants expressing either the TMV MP or the RCNMV MP, and a chimeric TMV that encodes the RCNMV MP as its only functional MP gene, to demonstrate that the MPs of TMV and RCNMV are functionally homologous. Further, both TMV and RCNMV can act as helper viruses to allow the cell-to-cell movement of the heterologous movement-defective viruses. These data support the conclusion that, despite other differences, such as particle morphology, host range, and sequence, TMV and RCNMV share a common mechanism for cell-to-cell movement
A comparison of two methods of microinjection for assessing altered plasmodesmal gating in tissues expressing viral movement proteins
Targeting and modification of prokaryotic cell–cell junctions by tobacco mosaic virus cell‐to‐cell movement protein
Evaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation
Advances in understanding recessive resistance to plant viruses
100 ref. doi: 10.1111/J.1364-3703.2004.00223.XInternational audienc