30 research outputs found
Avaliação de três áreas de solo construído após mineração de carvão a céu aberto em Lauro Müller, Santa Catarina
Tracing aquatic food webs using fatty acids: from qualitative indicators to quantitative determination
EFFECT OF VILLUS LENGTH ON CELL PROLIFERATION AND MIGRATION IN SMALL INTESTINAL EPITHELIUM
Effect of the physical nature of the culture medium on the metabolism of benzyladenine and endogenous cytokinins in Actinida deliciosa tissues cultured in vitro
Measuring species richness on sandy beach transects: extrapolative estimators and their implications for sampling effort
An Analysis of the Relationship between Transport Capital and Tourism Development in a Dynamic Framework
The RepA protein of plasmid pSC101 controls Escherichia coli cell division through the SOS response
Epigenomic and transcriptional profiling identifies impaired glyoxylate detoxification in NAFLD as a risk factor for hyperoxaluria
Epigenetic modifications (e.g. DNA methylation) in NAFLD and their contribution to disease progression and extrahepatic complications are poorly explored. Here, we use an integrated epigenome and transcriptome analysis of mouse NAFLD hepatocytes and identify alterations in glyoxylate metabolism, a pathway relevant in kidney damage via oxalate release—a harmful waste product and kidney stone-promoting factor. Downregulation and hypermethylation of alanine-glyoxylate aminotransferase (Agxt), which detoxifies glyoxylate, preventing excessive oxalate accumulation, is accompanied by increased oxalate formation after metabolism of the precursor hydroxyproline. Viral-mediated Agxt transfer or inhibiting hydroxyproline catabolism rescues excessive oxalate release. In human steatotic hepatocytes, AGXT is also downregulated and hypermethylated, and in NAFLD adolescents, steatosis severity correlates with urinary oxalate excretion. Thus, this work identifies a reduced capacity of the steatotic liver to detoxify glyoxylate, triggering elevated oxalate, and provides a mechanistic explanation for the increased risk of kidney stones and chronic kidney disease in NAFLD patients
Avaliação nutricional da silagem de grãos úmidos de milho com diferentes teores de óleo para leitões na fase de creche Nutritional evaluation of high moisture corn silage with different oil contents for piglets in nursery phase
Utilizaram-se 60 leitões mestiços (Large White x Landrace), desmamados com peso inicial médio de 7,9kg, no experimento de desempenho e 20 leitões mestiços, com peso inicial médio de 16,8kg, no experimento de digestibilidade para avaliar a silagem de grãos úmidos de milho com diferentes teores de óleo. O delineamento experimental foi o de blocos ao acaso em ambos os experimentos avaliou-se o valor nutricional das silagens e dos milhos secos com teor normal (4,3%EE na MS) ou elevado de óleo (5,66%EE na MS). Não houve efeito dos tratamentos sobre o consumo diário de ração e no ganho de peso diário nos períodos de 0 a 9 e 0 a 31 dias. Os leitões apresentaram melhor conversão alimentar em ambos os períodos estudados, quando receberam silagem e no período de 0 a 9 dias, quando foram alimentados com milho com teor mais alto de óleo. As frações digestível e metabolizável da energia foram influenciadas pelo processamento, sendo que a ensilagem proporcionou melhor aproveitamento da energia, independentemente do teor de óleo presente nos grãos.<br>Sixty crossbred weaned pigs (Large White x Landrace) average initial body weight of 7.9kg and 20 crossbred pigs average initial body weight of 16.8kg were used in the performance and digestibility experiments, respectively, to evaluate high-moisture corn silage with different oil contents. The nutritional value of high-moisture corn silage and dry corn with normal (4.3% EE in DM) or higher (5.66% EE in DM) oil contents were analyzed. The treatments had no effect on either average daily feed intake (ADFI) or average daily gain (ADG) from 0 to 9 and 0 to 31 days. The piglets had higher feed/weight gain in both periods when fed silage and in the period from 0 to 9 days when fed higher oil content corn. In the digestibility experiment only the digestible and metabolizable fractions of energy were affected by the processing method and the silage provided higher energy utilization regardless of the oil content in the grains