Stability indicating method development and validation for the determination of haloperidol and benzhexol by RP-HPLC

A simple, Accurate, precise method was developed for the simultaneous estimation of the Haloperidol and Benzhexol in Tablet dosage form. The chromatogram was run through Kromasil (250mm 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrile and methanol in the ratio of 48:52 was pumped through column at a flow rate of 1.0 ml/min. The temperature was maintained at 30°C. The optimized wavelength for Haloperidol and Benzhexol was 220nm. The retention time of Haloperidol and Benzhexol were found to be 2.415 min and 2.820min. %RSD of the Haloperidol and Benzhexol were and found to be 0.6 and 0.2 respectively. %Recover was Obtained as 98.92% and 99.60% for Haloperidol and Benzhexol. LOD, LOQ values were obtained from regression equations of Haloperidol and Benzhexol were 0.42ppm, 1.27ppm and 0.04ppm, 0.14ppm respectively. Regression equation of Haloperidol is y = 24009x + 38704, and of Benzhexol is y = 40558x + 2880. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industrie

Cardioprotective effect of pulicaria wightania against isoproterenol induced myocardial infarction in experimental rats

The current study was carried out to evaluate the cardio protective activity of Pulicaria Wightiana against Isoproterenol (ISO) induced myocardial infarction (MI). Pretreatment to the different groups were given for 30 days and ISO was administered at last two days with an interval of 24 hrs. Due to chronic ionotropy ISO induces MI. Blood was collected at the last day of experimental period and biomarkers were observed. The results indicate that the extract exhibited significant cardioprotective activity