Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2015

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 32 commercial cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2015 growing season in a randomized complete block design with 6 replications. At harvest on 28-29 September 2015, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 136 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 8 to 81%, weight loss ranged from 8.0 to 21.0%, sucrose losses ranged from 25 to 89%, and estimated recoverable sucrose ranged from 439 to 8,261 lb/A. Given these response ranges, selecting cultivars for rhizomania resistance and combining this resistance with storability will lead to considerable economic benefit for the sugar beet industry

Ionized Reflection Spectra from Accretion Disks Illuminated by X-Ray Pulsars

X-ray reflection signatures are observed around multiple classes of accreting compact objects. Modelling these features yield important constraints on the physics of accretion disks, motivating the development of X-ray reflection models appropriate for a variety of systems and illumination conditions. Here, constant density ionized X-ray reflection models are presented for a disk irradiated with a very hard power-law X-ray spectrum (\Gamma \u3c 1) and a variable high-energy cutoff. These models are then applied to the Suzaku data of the accreting X-ray pulsar LMC X-4, where very good fits are obtained with a highly ionized reflector responsible for both the broad Fe K line and the soft excess. The ionized reflector shows strong evidence for significant Doppler broadening and is redshifted by ~10^4 km/s. These features indicate that the reflecting material is associated with the complex dynamics occurring at the inner region of the magnetically-truncated accretion disk. Thus, reflection studies of X-ray pulsar spectra may give important insights into the accretion physics at the magnetospheric radius

Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2014

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 30 experimental cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2014 growing season in a randomized complete block design with 6 replications. At harvest on 24-25 September 2014, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 138 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 6 to 82%, weight loss ranged from 9.1 to 17.5%, sucrose losses ranged from 22 to 85%, and estimated recoverable sucrose ranged from 1,008 to 8,292 lb/A. Given these response ranges, selecting cultivars for rhizomania resistance and combining this resistance with storability will lead to considerable economic benefit for the sugar beet industry

Commercial sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2014

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 33 commercial cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2014 growing season in a randomized complete block design with 6 replications. At harvest on 24-25 September 2014, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 138 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 7 to 82%, weight loss ranged from 9.4 to 19.1%, sucrose losses ranged from 23 to 85%, and estimated recoverable sucrose ranged from 931 to 8,798 lb/A. Given these response ranges, selecting cultivars for rhizomania resistance and combining this resistance with storability will lead to considerable economic benefit for the sugar beet industry

Seed Transmission of the \u3ci\u3eHigh Plains virus\u3c/i\u3e in Sweet Corn

The High Plains virus (HPV), which infects corn and other cereals, was first found in 1993 in the United States. Research was initiated in 1995 to investigate the potential for seed transmission of HPV. Sweet corn seeds of various cultivars harvested in 1994 to 1996 from 13 fields and research plots in southwestern Idaho, Colorado, and Nebraska were seeded in potting mix in the greenhouse. Leaf samples collected at the three- to six-leaf stage from both symptomatic and asymptomatic plants were tested by enzyme-linked immunosorbent assay (ELISA). Of the 46,600 seeds planted, 38,473 seedlings emerged, and three tested positive by ELISA, exhibited mosaic symptoms, and had the presence of HPV confirmed by an additional test. One of the positive plants was used for successful acquisition and transmission of HPV by the wheat curl mite to Westford barley. The other two plants were used to successfully transfer HPV to other corn plants by vascular puncture inoculation of seed. These results indicate that HPV can be seed transmitted at a very low frequency in sweet corn

Evaluation of fungicide and biological treatments for control of fungal storage rots in sugar beet, 2014

Preventing sucrose losses in storage is important to the economic viability of the sugar beet industry. In an effort to establish additional measures for reducing sucrose losses in storage, ten fungicide and/or biological treatments were evaluated on sugar beet roots in a commercial sugar beet storage building for their ability to limit fungal growth on roots harvested 2 Oct. Six of the treatments were applied as a direct spray to roots, but two treatments were applied as a cold fog and two others were applied as a thermal fog. The treated eight-beet root samples were arranged in a randomized complete block design with 6 replications on top of the commercial sugar beet pile inside a storage building. Roots were evaluated for fungal growth, root rot, weight loss, and sucrose reduction. Fungal growth on the root surface ranged from 0 to 58% depending on the rating date and treatment. After 136 days in storage, root rot ranged from 4 to 34%, weight loss ranged from 7.5 to 10.2%, and sucrose reduction ranged from 17 to 33%. The treatments that reduced rot and sucrose reduction the most were Phostrol, Propulse, and Stadium applied as direct sprays and Propulse as a cold fog. Thus, the results indicate that several of the fungicides evaluated have the potential to protect roots from fungal rot in sugar beet storage piles, which could lead to considerable economic benefit for the sugar beet industry

The Broad-band Counterpart of the Short GRB 200522A at z=0.5536z=0.5536:A Luminous Kilonova or a Collimated Outflow with a Reverse Shock?

We present the discovery of the radio afterglow and near-infrared (NIR) counterpart of the Swift short GRB 200522A, located at a small projected offset of $\approx 1$ kpc from the center of a young, star-forming host galaxy at $z=0.5536$. The radio and X-ray luminosities of the afterglow are consistent with those of on-axis cosmological short GRBs. The NIR counterpart, revealed by our HST observations at a rest-frame time of $\approx2.3$ days, has a luminosity of $\approx (1.3-1.7) \times 10^{42}$ erg s$^{-1}$. This is substantially lower than on-axis short GRB afterglow detections, but is a factor of $\approx 8$-$17$ more luminous than the kilonova of GW170817, and significantly more luminous than any kilonova candidate for which comparable observations exist. The combination of the counterpart's color ($i-y = -0.08\pm 0.21$; rest-frame) and luminosity cannot be explained by standard radioactive heating alone. We present two scenarios to interpret the broad-band behavior of GRB 200522A: a synchrotron forward shock with a luminous kilonova (potentially boosted by magnetar energy deposition), or forward and reverse shocks from a $\approx14^{\circ}$, relativistic ($\Gamma_0 \gtrsim 80$) jet. Models which include a combination of enhanced radioactive heating rates, low-lanthanide mass fractions, or additional sources of heating from late-time central engine activity may provide viable alternate explanations. If a stable magnetar was indeed produced in GRB 200522A, we predict that late-time radio emission will be detectable starting $\approx 0.3$-$6$ years after the burst for a deposited energy of $\approx 10^{53}$ erg. Counterparts of similar luminosity to GRB 200522A associated with gravitational wave events will be detectable with current optical searches to $\approx\!250$ Mpc

The Broad-band Counterpart of the Short GRB 200522A at z=0.5536z=0.5536:A Luminous Kilonova or a Collimated Outflow with a Reverse Shock?

We present the discovery of the radio afterglow and near-infrared (NIR) counterpart of the Swift short GRB 200522A, located at a small projected offset of $\approx 1$ kpc from the center of a young, star-forming host galaxy at $z=0.5536$. The radio and X-ray luminosities of the afterglow are consistent with those of on-axis cosmological short GRBs. The NIR counterpart, revealed by our HST observations at a rest-frame time of $\approx2.3$ days, has a luminosity of $\approx (1.3-1.7) \times 10^{42}$ erg s$^{-1}$. This is substantially lower than on-axis short GRB afterglow detections, but is a factor of $\approx 8$-$17$ more luminous than the kilonova of GW170817, and significantly more luminous than any kilonova candidate for which comparable observations exist. The combination of the counterpart's color ($i-y = -0.08\pm 0.21$; rest-frame) and luminosity cannot be explained by standard radioactive heating alone. We present two scenarios to interpret the broad-band behavior of GRB 200522A: a synchrotron forward shock with a luminous kilonova (potentially boosted by magnetar energy deposition), or forward and reverse shocks from a $\approx14^{\circ}$, relativistic ($\Gamma_0 \gtrsim 80$) jet. Models which include a combination of enhanced radioactive heating rates, low-lanthanide mass fractions, or additional sources of heating from late-time central engine activity may provide viable alternate explanations. If a stable magnetar was indeed produced in GRB 200522A, we predict that late-time radio emission will be detectable starting $\approx 0.3$-$6$ years after the burst for a deposited energy of $\approx 10^{53}$ erg. Counterparts of similar luminosity to GRB 200522A associated with gravitational wave events will be detectable with current optical searches to $\approx\!250$ Mpc.Comment: 33 pages, 13 figures, 5 tables. Submitted to AAS Journal

An association study on contrasting cystic fibrosis endophenotypes recognizes KRT8 but not KRT18 as a modifier of cystic fibrosis disease severity and CFTR mediated residual chloride secretion

<p>Abstract</p> <p>Background</p> <p>F508del-CFTR, the most frequent disease-causing mutation among Caucasian cystic fibrosis (CF) patients, has been characterised as a mutant defective in protein folding, processing and trafficking. We have investigated the two neighbouring cytokeratin genes <it>KRT8 </it>and <it>KRT18 </it>in a candidate gene approach to ask whether variants in <it>KRT8 </it>and/or <it>KRT18 </it>modify the impaired ion conductance known as the CF basic defect, and whether they are associated with correct trafficking of mutant CFTR and disease severity of CF.</p> <p>Methods</p> <p>We have selected contrasting F508del-<it>CFTR </it>homozygous patient subpopulations stratified for disease severity, comparing 13 concordant mildly affected sib pairs vs. 12 concordant severely affected sib pairs, or manifestation of the CF basic defect in intestinal epithelium, comparing 22 individuals who exhibit CFTR-mediated residual chloride secretion vs. 14 individuals who do not express any chloride secretion, for an association. The <it>KRT8</it>/<it>KRT18 </it>locus was initially interrogated with one informative microsatellite marker. Subsequently, a low density SNP map with four SNPs in KRT8 and two SNPs in KRT18, each selected for high polymorphism content, was used to localize the association signal.</p> <p>Results</p> <p><it>KRT8</it>, but not <it>KRT18</it>, showed an association with CF disease severity (P_best= 0.00131; P_corr= 0.0185) and CFTR mediated residual chloride secretion (P_best= 0.0004; P_corr= 0.0069). Two major four-marker-haplotypes spanning 13 kb including the entire <it>KRT8 </it>gene accounted for 90% of chromosomes, demonstrating strong linkage disequilibrium at that locus. Absence of chloride secretion was associated with the recessive haplotype 1122 at rs1907671, rs4300473, rs2035878 and rs2035875. The contrasting haplotype 2211 was dominant for the presence of CFTR mediated residual chloride secretion. In consistency, the <it>KRT8 </it>haplotype 2211 was associated with mild CF disease while 1122 was observed as risk haplotype. Analysis of microsatellite allele distributions on the SNP background suggests that the mild <it>KRT8 </it>haplotype 2211 is phylogenetically older than its severe counterpart.</p> <p>Conclusions</p> <p>The two opposing <it>KRT8 </it>alleles which have been identified as a benign and as a risk allele in this work are likely effective in the context of epithelial cell differentiation. As the mild <it>KRT8 </it>allele is associated with CFTR mediated residual chloride secretion among F508del-<it>CFTR </it>homozygotes, the KRT8/KRT18 heterodimeric intermediary filaments of the cytoskeleton apparently are an essential component for the proper targeting of CFTR to the apical membrane in epithelial cells.</p

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

Anthracnose and bean common mosaic (BCM) are considered major diseases in common bean crop causing severe yield losses worldwide. This work describes the introgression and pyramiding of genes conferring genetic resistance to BCM and anthracnose local races into line A25, a bean genotype classified as market class fabada. Resistant plants were selected using resistance tests or combining resistance tests and marker-assisted selection. Lines A252, A321, A493, Sanilac BC6-Are, and BRB130 were used as resistance sources. Resistance genes to anthracnose (Co-2 ( C ), Co-2 ( A252 ) and Co-3/9) and/or BCM (I and bc-3) were introgressed in line A25 through six parallel backcrossing programs, and six breeding lines showing a fabada seed phenotype were obtained after six backcross generations: line A1258 from A252; A1231 from A321; A1220 from A493; A1183 and A1878 from Sanilac BC6-Are; and line A2418 from BRB130. Pyramiding of different genes were developed using the pedigree method from a single cross between lines obtained in the introgression step: line A1699 (derived from cross A1258 × A1220), A2438 (A1220 × A1183), A2806 (A1878 × A2418), and A3308 (A1699 × A2806). A characterization based on eight morpho-agronomic traits revealed a limited differentiation among the obtained breeding lines and the recurrent line A25. However, using a set of seven molecular markers linked to the loci used in the breeding programs it was possible to differentiate the 11 fabada lines. Considering the genetic control of the resistance in resistant donor lines, the observed segregations in the last backcrossing generation, the reaction against the pathogens, and the expression of the molecular markers it was also possible to infer the genotype conferring resistance in the ten fabada breeding lines obtained. As a result of these breeding programs, genetic resistance to three anthracnose races controlled by genes included in clusters Co-2 and Co-3/9, and genetic resistance to BCM controlled by genotype I + bc-3 was combined in the fabada line A3308