Immunocytochemical Labeling of Enzymes in Low Temperature Embedded Plant Tissue: The Precursor of Glyoxysomal Malate Dehydrogenase is Located in the Cytosol of Watermelon Cotyledon Cells

The Lowicryl-technique in combination with protein A gold was used in order to localize the precursor of glyoxysomal malate dehydrogenase in watermelon cotyledons. Preservation of the antigen was evaluated by a preembedding technique in isolated organelles. The glyoxysomal malate dehydrogenase was localized in tissue sections by a postembedding technique. Antigens of glyoxysomal malate dehydrogenase were found in the glyoxysomal matrix and in the cytosol, whereas the endoplasmic reticulum was completely free of labeling. Controls are presented by preimmunserum, by a serum against various proteins of the glyoxysomal membrane and by application of cycloheximide in order to inhibit translation at cytosolic ribosomes. The results are compared with immunocytochemical localizations of other plant microbody enzymes and of plant storage proteins

Northwest Africa 5790. Top Sequence of the Nakhlite Pile

NWA 5790 is a recently discovered nakhlite. Its mineralogy, petrology and geochemistry suggest that it is the topmost sequence of the nakhlite lava pile

Biofortification of essential nutritional compounds and trace elements in rice and cassava

Plant biotechnology can make important contributions to food security and nutritional improvement. For example, the development of ‘Golden Rice' by Professor Ingo Potrykus was a milestone in the application of gene technology to deliver both increased nutritional qualities and health improvement to wide sections of the human population. Mineral nutrient and protein deficiency as well as food security remain the most important challenges for developing countries. Current projects are addressing these issues in two major staple crops, cassava (Manihot esculenta Crantz) and rice. The tropical root crop cassava is a major source of food for approximately 600 million of the population worldwide. In sub-Saharan Africa >200 million of the population rely on cassava as their major source of dietary energy. The nutritional quality of the cassava root is not sufficient to meet all dietary needs. Rice is the staple food for half the world population, providing approximately 20% of the per capita energy and 13% of the protein for human consumption worldwide. In many developing countries the dietary contributions of rice are substantially greater (29·3% dietary energy and 29·1% dietary protein). The current six most popular ‘mega' rice varieties (in terms of popularity and acreage), including Chinese hybrid rice, have an incomplete amino acid profile and contain limited amounts of essential micronutrients. Rice lines with improved Fe contents have been developed using genes that have functions in Fe absorption, translocation and accumulation in the plant, as well as improved Fe bioavailability in the human intestine. Current developments in biotechnology-assisted plant improvement are reviewed and the potential of the technology in addressing human nutrition and health are discusse

Are earthworm populations maintained in public parks in cities? The case of Curitiba, Paraná, Brazil.

ISEE 10

Enchytraeus crypticus em ensaios ecotoxicológicos com solo artificial tropical.

ECOTOX. Poster 544

Microanelídeos como indicadores de qualidade do solo em parques urbanos em Curitiba - PR.

Resumo. FERTBIO 2014

Efeito de uma formulação comercial de glifosato sobre a reprodução de uma espécie nativa de enquitreídeo (Enchytraeidae, Oligochaeta).

ECOTOX. Poster 537

Enchytraeids (Oligochaeta, Enchytraeidae) as soil quality indicators in integrated systems in Paraná State.

Resumo