GPR171 Modulates Mood, Consummatory Behaviors, And Morphine Antinociception in a Sexually Dimorphic Pattern in Mice

Receptors in the brain influence everything from complex behaviors related to mood, all the way to simple physiological functions like the way a person moves. Receptors are activated or inactivated by chemicals or hormones that the body produces or that are created to mimic the body’s natural chemicals. Of the hundreds of receptors in the brain, GPR171 is particularly interesting because new drugs have been created to activate or block the receptor, and are being proposed for the treatment of different disorders, particularly disorders related to pain. GPR171 has been shown to affect pain behaviors, eating, and mood-related behaviors, but has not been well researched beyond these few studies. In addition, it is not clear what GPR171 does in females, as they have not been included in most of the research of the receptor. In our research, we explored how GPR171 affects females, particularly in anxiety, depression, and stress, to better understand its role. We also used a new method involving mice genetically modified to lack GPR171 to investigate its broader influence on behaviors such as eating, body functions, mood, and pain, directly comparing the results between male and female mice. Our findings show that blocking GPR171 in female mice reduced anxiety-like behaviors and was influenced by estrogen, suggesting that hormones may change how GPR171 works. We also show that mice without GPR171 displayed changes in eating behavior, movement coordination, anxiety, and depression, which were dependent on the sex of the mice. Additionally, these modified mice showed a reduced response to morphine, a common pain medication, confirming that GPR171 is necessary for morphine to work properly. Overall, this dissertation underscores the importance of GPR171 in controlling various behaviors and bodily functions. It shows that this receptor is essential for normal functioning and that its effects can differ significantly between males and females. This highlights the need to consider these differences as treatments targeting this receptor for various medical conditions are being developed

Occupational radiation exposure at commercial nuclear power reactors and other facilities 1992; Twenty-fifth annual report, Volume 14

This report summarizes the occupational radiation exposure information that has been reported to the NRC`s Radiation Exposure Information Reporting System (REIRS) by nuclear power facilities and certain other categories of NRC licensees during the years 1969 through 1992. The bulk of the data presented in the report was obtained from annual radiation exposure reports submitted in accordance with the requirements of 10CFR20.407 and the technical specifications of nuclear power plants. Data on workers terminating their employment at certain NRC licensed facilities were obtained from reports submitted pursuant to 10CFR20.408. The 1992 annual reports submitted by about 364 licensees indicated that approximately 204,365 individuals were monitored, 183,927 of whom were monitored by nuclear power facilities. They incurred an average individual dose of 0.16 rem (cSv) and an average measurable dose of about 0.30 (cSv). Termination radiation exposure reports were analyzed to reveal that about 74,566 individuals completed their employment with one or more of the 364 covered licensees during 1992. Some 71,846 of these individuals terminated from power reactor facilities, and about 9,724 of them were considered to be transient workers who received an average dose of 0.50 rem (cSv)

Land contributions to natural CO2 variability on time scales of centuries

The present paper addresses the origin of natural variability arising internally from the climate system of the global carbon cycle at centennial time scales. The investigation is based on the Max Planck Institute for Meteorology, Coupled Model Intercomparison Project Phase 5 (MPI-MCMIP5) preindustrial control simulations with the MPI Earth System Model in low resolution (MPI-ESM-LR) supplemented by additional simulations conducted for further analysis. The simulations show a distinct low-frequency component in the global terrestrial carbon content that induces atmospheric CO2 variations on centennial time scales of up to 3 ppm. The main drivers for these variations are low-frequency fluctuations in net primary production (NPP) of the land biosphere. The signal arises from small regions scattered across the whole globe with a pronounced source in North America. The main reason for the global NPP fluctuations is found in climatic changes leading to long-term variations in leaf area index, which largely determines the strength of photosynthetic carbon assimilation. The underlying climatic changes encompass several spatial diverse climatic alterations. For the particular case of North America, the carbon storage changes are (besides NPP) also dependent on soil respiration. This second mechanism is strongly connected to low-frequency variations in incoming shortwave radiation at the surface. ©2013. American Geophysical Union. All Rights Reserved

Two drastically different climate states on an Earth-like terra-planet

We study an Earth-like terra-planet (water-limited terrestrial planet) with an overland recycling mechanism bringing fresh water back from the high latitudes to the low latitudes. By performing model simulations for such a planet we find two drastically different climate states for the same set of boundary conditions and parameter values: a cold and wet (CW) state with dominant low-latitude precipitation and a hot and dry (HD) state with only high-latitude precipitation. We notice that for perpetual equinox conditions, both climate states are stable below a certain threshold value of background soil albedo while above the threshold only the CW state is stable. Starting from the HD state and increasing background soil albedo above the threshold causes an abrupt shift from the HD state to the CW state resulting in a sudden cooling of about 35 °C globally, which is of the order of the temperature difference between present day and the Snowball Earth state. When albedo starting from the CW state is reduced down to zero the terra-planet does not shift back to the HD state (no closed hysteresis). This is due to the high cloud cover in the CW state hiding the surface from solar irradiation so that surface albedo has only a minor effect on the top of the atmosphere radiation balance. Additional simulations with present-day Earth's obliquity all lead to the CW state, suggesting a similar abrupt transition from the HD state to the CW state when increasing obliquity from zero. Our study also has implications for the habitability of Earth-like terra-planets. At the inner edge of the habitable zone, the higher cloud cover in the CW state cools the planet and may prevent the onset of a runaway greenhouse state. At the outer edge, the resupply of water at low latitudes stabilizes the greenhouse effect and keeps the planet in the HD state and may prevent water from getting trapped at high latitudes in frozen form. Overall, the existence of bistability in the presence of an overland recycling mechanism hints at the possibility of a wider habitable zone for Earth-like terra-planets at low obliquities

Past land use decisions have increased mitigation potential of reforestation

Anthropogenic land cover change (ALCC) influences global mean temperatures via counteracting effects: CO2 emissions contribute to global warming, while biogeophysical effects, in particular the increase in surface albedo, often impose a cooling influence. Previous studies of idealized, large-scale deforestation found that albedo cooling dominates over CO 2 warming in boreal regions, indicating that boreal reforestation is not an effective mitigation tool. Here we show the importance of past land use decisions in influencing the mitigation potential of reforestation on these lands. In our simulations, CO2 warming dominates over albedo cooling because past land use decisions resulted in the use of the most productive land with larger carbon stocks and less snow than on average. As a result past land use decisions extended CO2 dominance to most agriculturally important regions in the world, suggesting that in most places reversion of past land cover change could contribute to climate change mitigation. While the relative magnitude of CO2 and albedo effects remains uncertain, the historical land use pattern is found to be biased towards stronger CO2 and weaker albedo effects as compared to idealized large-scale deforestation. Copyright 2011 by the American Geophysical Union

Biogeophysical versus biogeochemical climate response to historical anthropogenic land cover change

Anthropogenic land cover change (ALCC) is one of the few climate forcings with still unknown sign of their climate response. Major uncertainty results from the often counteracting temperature responses to biogeochemical as compared to biogeophysical effects. Here, we separate the strength of these two effects for ALCC during the last millennium. We add unprecedented detail by (i) using a coupled atmosphere/ocean general circulation model (GCM), and (ii) applying a high-detail reconstruction of historical ALCC. We find that biogeophysical effects have a slight cooling influence on global mean temperature (-0.03 K in the 20th century), while biogeochemical effects lead to strong warming (0.16-0.18 K). During the industrial era, both effects cause significant changes in certain regions; only few regions, however, experience biogeophysical cooling strong enough to dominate the overall temperature response. This study therefore suggests that the climate response to historical ALCC, both globally and in most regions, is dominated by the rise in CO2 caused by ALCC emissions

Contribution of anthropogenic land cover change emissions to preindustrial atmospheric CO2

Based on a recent reconstruction of anthropogenic land cover change (ALCC), we derive the associated CO2 emissions since 800 AD by two independent methods: a bookkeeping approach and a process model. The results are compared with the pre-industrial development of atmospheric CO2 known from antarctic ice cores. Our results show that pre-industrial CO2 emissions from ALCC have been relevant for the pre-industrial carbon cycle, although before 1750 AD their trace in atmospheric CO2 is obscured by other processes of similar magnitude. After 1750 AD, the situation is different: the steep increase in atmospheric CO2 until 1850 AD-this is before fossil fuel emissions rose to significant values-is to a substantial part explained by growing emissions from ALCC. © 2010 The Authors Tellus B © 2010 International Meteorological Institute in Stockholm

Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea : research article

Background Differential expression of genes can be regulated on many different levels. Most global studies of gene regulation concentrate on transcript level regulation, and very few global analyses of differential translational efficiencies exist. The studies have revealed that in Saccharomyces cerevisiae, Arabidopsis thaliana, and human cell lines translational regulation plays a significant role. Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available. Results A global analysis of translational control was performed with two haloarchaeal model species, Halobacterium salinarum and Haloferax volcanii. To identify differentially regulated genes, exponentially growing and stationary phase cells were compared. More than 20% of H. salinarum transcripts are translated with non-average efficiencies. By far the largest group is comprised of genes that are translated with above-average efficiency specifically in exponential phase, including genes for many ribosomal proteins, RNA polymerase subunits, enzymes, and chemotaxis proteins. Translation of 1% of all genes is specifically repressed in either of the two growth phases. For comparison, DNA microarrays were also used to identify differential transcriptional regulation in H. salinarum, and 17% of all genes were found to have non-average transcript levels in exponential versus stationary phase. In H. volcanii, 12% of all genes are translated with non-average efficiencies. The overlap with H. salinarum is negligible. In contrast to H. salinarum, 4.6% of genes have non-average translational efficiency in both growth phases, and thus they might be regulated by other stimuli than growth phase. Conclusions For the first time in any prokaryotic species it was shown that a significant fraction of genes is under differential translational control. Groups of genes with different regulatory patterns were discovered. However, neither the fractions nor the identity of regulated genes are conserved between H. salinarum and H. volcanii, indicating that prokaryotes as well as eukaryotes use differential translational control for the regulation of gene expression, but that the identity of regulated genes is not conserved For 70 H. salinarum genes potentiation of regulation was observed, but for the majority of regulated genes either transcriptional or translational regulation is employed

Global biogeophysical interactions between forest and climate

In two sensitivity experiments using the Earth System Model of the Max Planck Institute for Meteorology (MPI‐ESM), the vegetation cover of the ice‐free land surface has been set worldwide to either forest or grassland in order to quantify the quasi‐equilibrium response of the atmosphere and ocean components to extreme land surface boundary conditions. After 400 years of model integration, the global mean annual surface temperature increased by 0.7°K and declined by 0.6°K in the forest and grassland simulations, respectively, as compared to the control simulation. Thereafter, the geographic distribution of vegetation has been allowed to respond interactively to climate. After subsequent 500 years of interactive climate‐vegetation dynamics, both forest and grassland simulations converged to essentially the same climate state as in the control simulation. This convergence suggests an absence of multiple climate‐forest states in the current version of the MPI‐ESM

Radiative forcing from anthropogenic land cover change since AD 800

We calculate the radiative forcing (RF) from surface albedo changes over the last millennium applying a recently published, population-based reconstruction of anthropogenic land cover change (ALCC). This study thus allows for the first time to assess anthropogenic effects on climate during the pre-industrial era at high spatial and temporal detail. We find that the RF is small throughout the pre-industrial period on the global scale (negative with a magnitude less than 0.05 W/m2) and not strong enough to explain the cooling reconstructed from climate proxies between A.D. 1000 and 1900. For the regional scale, however, our results suggest an early anthropogenic impact on climate: Already in A.D. 800, the surface energy balance was altered by ALCC at a strength comparable to present-day greenhouse gas forcing, e.g., −2.0 W/m2 are derived for parts of India for that time. Several other regions exhibit a distinct variability of RF as a result of major epidemics and warfare, with RF changes in the order of 0.1 W/m2 within just one century