Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathies

Journal ArticleCapillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples were analyzed with CZE and AGE to determine sensitivity and specificity in detecting TFE-confirmed monoclonal gammopathies. Both techniques detected all monoclonal spikes due to lgM (n - 8), igG (n = 38), and free light chains (n = 3). Agarose gel electrophoresis, however, detected only 11 of 1.4 (79%) IgA monoclonal spikes detected with CZE. Jn a second study, 78 serum samples, 48 of which had a monoclonal gammopathy confirmed with IFE, were evaluated with ISE. Only 60% to 75% of the monoclonal gammopathies were correctly immunotyped with ISE by 4 readers blinded to the IFE immunotype. Thus CZE was more sensitive than AGE in detecting low concentrations of monoclonal proteins, but ISE is less accurate than IFE in determining the immunotype of the monoclonal gammopathy

Project-Based Learning (Pjbl) in Three Southeastern Public Schools: Academic, Behavioral, and Social-Emotional Outcomes

Project-based learning (PjBL) as a PK–12 instructional model is growing nationwide. PjBL is seen as a mechanism to deliver academic content in a more engaging way for students and in a way that stresses the development of skills critical to success in the 21st-century workforce. Because of its increasing popularity and the disparate breadth of research around the model, a study of PjBL in three southeastern public schools was conducted during academic years 2015–2016 and 2016–2017. This study attempted to better understand how PjBL was implemented in schools and to explore the impact of PjBL on schools, teachers, and students. Data collection included classroom observations, educator surveys, student surveys, and an analysis of academic and behavioral outcomes and a subset of social-emotional skills. Findings did not reveal consistent significant differences in the performances of PjBL and non-PjBL demographically matched students on academic and behavioral outcomes. PjBL students did, however, perform better on inventories of social-emotional skills. In addition, while PjBL implementation challenges are apparent, perceptions of students and educators of the impact and possibilities of PjBL are quite positive

From Mothering Without a Net to Mothering on the Net: The Impact of an Online Social Networking Site on Experiences of Postpartum Depression

This research speaks to the desire expressed by women, health care professionals, and researchers alike, for an alternative, non-pharmacological or therapy-related intervention for postpartum depression (PPD). Interviews with twenty-two mothers and members of Momstown.ca—a social networking site focused on connecting mothers online and face-to-face—demonstrated new mothers are increasingly isolated and without community. However, mothers reported becoming members of Momstown allowed them to build a social network providing them company, camaraderie, and community, resulting in improved mental health. We conclude services like Momstown could be viable, alternative interventions for women with ppd, enabling mothers to survive and thrive postpartum

Low Apparent Survival and Heterogeneous Movement Patterns of Invasive Blue Catfish in a Coastal River

Blue Catfish Ictalurus furcatus were purposefully introduced into freshwater tributaries to Chesapeake Bay in the past, and populations have subsequently spread to new areas, negatively impacting native communities and causing concern for resource managers. To aid development of management strategies, we implemented a multiyear (2012-2015) tagging study of invasive Blue Catfish in a 40-km stretch of the Potomac River to estimate survival and assess movement patterns. Blue Catfish (N = 1,237) were captured by electrofishing and double-tagged to allow us to estimate tag retention rates; we used reward tags to increase reporting rates. Recaptured fish (N = 104; 8.4% return rate) were at large for between 2 and 1,208 d. Tag retention rates were 0.88 (SE = 0.045) after 1 year and declined to 0.31 (SE = 0.107) after 2.7 years. The mean minimum distance moved by fish was 24.1 km (range = 0.0-112.6 km). Most (63%) fish displayed downriver movements, but distance moved was unrelated to fish size or days at large. Greater distances were observed among fish that moved downriver (34.4 km) than those that moved upriver (6.7 km). These results suggest high variability in movement behaviors for Blue Catfish inhabiting the tidal Potomac River from freshwater reaches to estuarine habitats. We estimated an annual apparent survival rate of 0.56 (SE = 0.057; Brownie tag-return model) across the study period. This survival rate is lower than survival rates reported from their native range. Long-distance movements of Blue Catfish in the Potomac River indicate that robust, large-scale control measures will be needed to reduce population abundance and minimize negative impacts of this species on native communities

The cavity-chaperone Skp protects its substrate from aggregation but allows independent folding of substrate domains

Outer membrane proteins (OMPs) of Gram-negative bacteria are synthesized in the cytosol and must cross the periplasm before insertion into the outer membrane. The 17-kDa protein (Skp) is a periplasmic chaperone that assists the folding and insertion of many OMPs, including OmpA, a model OMP with a membrane embedded β-barrel domain and a periplasmic αβ domain. Structurally, Skp belongs to a family of cavity-containing chaperones that bind their substrates in the cavity, protecting them from aggregation. However, some substrates, such as OmpA, exceed the capacity of the chaperone cavity, posing a mechanistic challenge. Here, we provide direct NMR evidence that, while bound to Skp, the β-barrel domain of OmpA is maintained in an unfolded state, whereas the periplasmic domain is folded in its native conformation. Complementary cross-linking and NMR relaxation experiments show that the OmpA β-barrel is bound deep within the Skp cavity, whereas the folded periplasmic domain protrudes outside of the cavity where it tumbles independently from the rest of the complex. This domain-based chaperoning mechanism allows the transport of β-barrels across the periplasm in an unfolded state, which may be important for efficient insertion into the outer membrane

Spatial Contours of Potential Biomass Crop Production: An Examination of Variations by U.S. Region

The recent and projected status of energy production and consumption in the United States, resulting in substantial dependencies upon foreign oil, has continued to provide pressure on domestic energy security. All told, bio-energy systems, and biomass crop production in particular, will be important elements of national security, economic vitality, and public policy. Using biomass crop estimates based upon models developed at the Oak Ridge National Laboratory and the Department of Energy (DOE) National Renewable Energy Laboratory, we identify potential biomass crop production zones using spatial analysis methods. The Midwest and the South are, by far, the largest regions of potential production. Once potential biomass crop yield is made proportional to estimated land and production costs, the South’s optimal crop zones fall along the Gulf Coast and Atlantic Seaboard, whereas in the Midwest, they are largely in non-metropolitan localities. The implications of these spatial contours for energy policy for alternative biomass crop production are discussed

Tracking Decadal Changes in Striped Bass Recruitment: A Calibration Study of Seine Surveys in Chesapeake Bay

In this study we estimated calibration factors necessary to maintain the long‐term integrity of the juvenile striped bass surveys in the Chesapeake Bay region. These surveys provide annual indices of recruitment (estimated as juvenile fish abundance in summer) and are used by fisheries managers in Virginia and Maryland to inform adjustments of annual harvest limits for striped bass from the commercial and recreational fisheries in Chesapeake Bay. During the multi‐decadal history of the survey, a potentially influential change occurred: VIMS deployed a net (the VA net) with a mesh material that differed from the standard net that MD DNR continued to deploy (the MD net). More recently, another change was necessitated when neither the standard net material nor the net material recently used by VIMS was available for construction of replacement nets. Hence, a net using new mesh material was constructed and experimentally deployed in 2015 (new net). Paired net hauls (n=144) were completed in Maryland and Virginia nursery areas during summer 2015 to permit estimation of calibration factors: 70 pairs with the VA‐MD nets, 42 pairs with the MD‐New nets, and 32 pairs with the VA‐new nets. Not all paired hauls captured a given target species, however. Three sets of calibration factors were estimated from using beta‐binomial models that accounted for differences in capture efficiencies and variation in the relative capture success of the nets. We considered the effects of several environmental covariates (e.g., temperature, salinity, and turbidity) as well as deployment characteristics (e.g., bottom type, calendar day, and maximum net extension) on the relative efficiency of nets and on the variation in the probability of capture among paired hauls. More..