Kemijski sastav endemske biljke Centaurea austro-anatolica i ispitivanje antimikrobnog djelovanja protiv multi-rezistentnih bakterija

Hexane, chloroform, ethyl acetate and ethanolic extracts of the aerial parts of C. austro-anatolica Hub.-Mor. (Asteraceae) were evaluated against microorganisms, including multi-resistant bacteria, using a paper disc diffusion method. The chloroform extract exhibited significant antibacterial activity toward all bacteria tested. The chemical composition of the chloroform extract was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major compounds of the extract were caryophyllene oxide (21.32 %), spathulenol (10.86 %), n-tricosanol (9.58 %) and geranyl isovalerate (8.71 %).Heksanski, kloroformski, etil-acetatni i etanolni ekstrakti vršnih dijelova biljke C. austro-anatolica Hub.-Mor. (Asteraceae) ispitivani su na antimikrobno djelovanje protiv multi-rezistentnih bakterija, koristeći difuzijsku metodu na papirnom disku. Kloroformski ekstrakt pokazao je značajno antibakterijsko djelovanje protiv svih testiranih bakterija. Kemijski sastav tog ekstrakta određivan je plinskom kromatografijom (GC) i plinskom kromatografijom-spektrometrijom masa (GC-MS). Najvažniji sastojci ekstrakta bili su kariofilen oksid (21,32 %), spatulenol (10,86 %), n-trikozanol (9,58 %) i geranil izovalerat (8,71 %)

Antispasmodic effect of Achillea nobilis L. subsp. sipylea (O. Schwarz) Bässler on the rat isolated duodenum

PubMed ID: 12648812The aim of the present study was to investigate the antispasmodic effect of the total extract of Achillea nobilis L. subsp. sipylea (Schwarz) Bässler (Asteraceae) on rat duodenum. In the first part of experiments, cumulative dose-response curves for acetylcholine (Ach) were obtained and then dose-response curves are repeated after addition of atropine, papaverine and different doses of the extract. In the second part, cumulative dose-response curves to CaCl2 were obtained in the absence and presence of verapamil and different doses of the extract. In the third part, papaverine and extract were applied to the tissues after contraction with K+. The extract has exhibited an inhibitory effect on the dose-response curves induced by Ach and CaCl2 on rat duodenum and significantly reduced the maximal response in a concentration-dependent manner. A similar effect was observed with papaverine but not with atropine on the dose-response curves obtained by ACh. Verapamil also reduced the maximal response in curves induced by CaCl2. The present results demonstrate that total extract of A. nobilis subsp. sipylea exerts antispasmodic activity on rat duodenum. © 2002 Elsevier Science Ireland Ltd. All rights reserved

Antispasmodic effect of Achillea nobilis L. subsp sipylea (O. Schwarz) Bassler on the rat isolated duodenum

WOS: 000180757500009PubMed ID: 12648812The aim of the present study was to investigate the antispasmodic effect of the total extract of Achillea nobilis L. subsp. sipylea (Schwarz) Bassler (Asteraceae) on rat duodenum. In the first part of experiments, cumulative dose-response curves for acetylcholine (Ach) were obtained and then dose-response curves are repeated after addition of atropine, papaverine and different doses of the extract. In the second part, cumulative dose-response curves to CaCl2 were obtained in the absence and presence of verapamil and different doses of the extract. In the third part, papaverine and extract were applied to the tissues after contraction with K+. The extract has exhibited an inhibitory effect on the dose-response curves induced by Ach and CaCl2 on rat duodenum and significantly reduced the maximal response in a concentration-dependent manner. A similar effect was observed with papaverine but not with atropine on the dose-response curves obtained by ACh. Verapamil also reduced the maximal response in curves induced by CaCl2. The present results demonstrate that total extract of A. nobilis subsp. sipylea exerts antispasmodic activity on rat duodenum. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved

Composition of essential oils of ten centaurea L. Taxa from Turkey

Essential oils obtained by microdistillation from seven endemic Centaurea species [C. hierapolitana Boiss., C. cadmea Boiss., C. calolepis Boiss., C. cariensis Boiss. subsp. maculiceps (O. Schwarz) Wagenitz, C. cariensis Boiss. subsp. microlepis (Boiss.) Wagenitz, C. ensiformis P.H. Davis and C. reuterana Boiss. var. reuterana] and three widespread (C. cyanus L., C. depressa Bieb. and C. urvillei DC. subsp. urvillei) Centaurea L. (Asteraceae) taxa from Turkey were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS) techniques. A total of 158 different compounds were identified. Significant and quantitative differences were observed among the species. Hexadecanoic acid (19.5–33.4%) was the main constituent of C. hierapolitana, C. cadmea, C. calolepis, C. reuterana var. reuterana, C. depressa and C. urvillei subsp. urvillei oils, while carvacrol (13.0–28.4%) was the major compound in, C. cariensis subsp. microlepis, C. cariensis subsp. maculiceps, C. ensiformis and C. cyanus oils. © 2008, Taylor & Francis Group, LLC. All rights reserved