Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs

The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy. Both antigens co-distribute with the chromatin in Sphase nuclei; however, as DNA replication is completed and nuclei progress into a G2 state anti-PCNA fluorescence disappears and anti-DNA polymerase alpha fluorescence becomes resolved into bright spots. These spots are initially associated with the chromatin strands and can be seen to share both anti-PCNA and anti-DNA polymerase alpha fluorescence, but as anti-PCNA fluorescence fades the spots become dissociated from the chromatin and are redistributed throughout the nucleus until they are dispersed during nuclear envelope breakdown. The loss of anti-PCNA fluorescence and displacement of anti-DNA polymerase alpha fluorescence from the chromatin can be prevented by inhibiting DNA synthesis 'with aphidicolin. Under these conditions both antigens remain associated 'with the chromatin even after nuclear envelope breakdown and lamin dispersal. The association of these antigens with mitotic figures appears to be functional, as both biotin-11-dUTP and pPJdCTP can be incorporated efficiently into DNA during the mitotic period

Could natural selection change the geographic range limits of light brown apple moth (Lepidoptera, Tortricidae) in North America?

We artificially selected for increased freeze tolerance in the invasive light brown apple moth. Our results suggest that, by not accounting for adaptation to cold, current models of potential geographic distributions could underestimate the areas at risk of exposure to this species

Evaluation of Experimental Populations and Glandular-Haired Varieties of Alfalfa for Alfalfa Blotch Leafminer (Diptera: Agromyzidae) Feeding Injury

Following the spread of the alfalfa blotch leafminer, Agromyza frontella (Rondani) (Diptera: Agromyzidae), into Minnesota and Wisconsin U.S.A. during 1994-1997, three field trials were conducted in Minnesota to assess the potential for leafminer resistance among several sources of alfalfa (Medicago sativa), germplasm. In 1998, 86 entries were evaluated, most of which were experimental populations. In addition, six commercial varieties of alfalfa were evaluated. Of the six varieties, four had been bred for various levels of glandular-hair expression, specifically for resistance to the potato leafhopper, Empoasca fabae (Harris) (Homoptera: Cicadellidae). In two of three trials, we found no significant differences in leafmining injury to trifoliolates among the 86 entries, or among glandular-haired and traditional commercial varieties. At one location, ‘Arrest,’ ‘Ameriguard 301,’ and ‘DK 121 HG’ incurred significantly less pinhole injury than the glandular-haired variety ‘5347 LH’ or the commercial standard, ‘5454.’ However, after accounting for both pinhole and leafmining injury, only ‘Arrest’ and ‘Ameriguard 301’ had less injury than ‘5347 LH,’ ‘DK 121 HG,’ or the standard ‘5454.’ The low levels of resistance to A. frontella injury, among glandular-haired commercial alfalfa varieties and numerous experimental populations M. sativa, confirm the need for alternative A. frontella management strategies such as biological control and possible manipulation of harvest schedules

Impact of Simulated Insect Defoliation and Timing of Injury on Cabbage Yield in Minnesota

In 1992 and 1994, field studies were done to assess the tolerance of transplanted cabbage to simulated insect defoliation and to determine if the defoliation level and growth stage at which defoliation begins influences final yield. In both years, 6 defoliation levels ranging from 0-100% were applied to transplanted cabbage at 4 time intervals. The time intervals began at transplanting, pre-head and head stages and continued until either head stage or harvest. For both years, the only time interval with significantly higher yield than the trans- plant to harvest interval (longest interval) was the head to harvest interval (shortest interval) and significant yield loss occurred only when defoliation was \u3e12.5%. Results suggest that transplanted cabbage can withstand relatively low levels of defoliation before yield loss occurs but that yield loss is also related to the duration over which defoliation occurs. In early growth stages, to protect yield, pest management practices should focus on reducing the interval over which damage occurs. The use of cultural practices that delay the onset of defoliation or allow avoidance of pests could protect yield. These strategies may include using transplants to shorten the time from planting to harvest or using planting dates that allow significant plant growth (i.e., head stage) before defoliators are able to infest the crop and cause significant damage. In addition, management strategies that reduce pest populations can also protect yield but at the head stage should switch to managing pests to protect marketability by reducing aesthetic damage and head contaminants

Early-Season Phenology and Temporal Dynamics of the Common Asparagus Beetle, \u3ci\u3eCrioceris Asparagi\u3c/i\u3e (Coleoptera: Chrysomelidae), in Southern Minnesota

During the years 1991-1994, studies were conducted to determine the early-season phenology and temporal dynamics of Crioceris asparagi (L.) (Co- leoptera: Chrysomelidae) in southern Minnesota asparagus. To document the early-season phenology, asparagus plots were sampled for egg, larval, and adult stages of C. asparagi during the months of May and June. Temporal dynamics of C. asparagi were determined by measuring the diurnal activity of adults and sampling asparagus plots at specific times (7 am, 9 am, 11 am, 1 pm, 3 pm and 5 pm) throughout May and June. We first detected C. asparagi adults in early May and they remained active throughout the sampling period. Eggs and larvae were also found; larval infestations on spears, however, were consistently lower than those for eggs. The temporal dynamics of C. asparagi adults showed that a higher percentage of asparagus plants were observed to be infested with beetles during the afternoon hours of 1 and 5 pm. The information provided in this paper illustrates the importance of determining the optimum time of day for sampling and will assist in properly targeting sampling efforts in future asparagus research and integrated pest management (IPM) programs

Strengthening the ethical assessment of placebo-controlled surgical trials : three proposals

Peer reviewedPublisher PD

Knowledge is Power: The Criminal Law, Openness and Privacy

Citizens deserve to know, and in some cases need to know, what their governments — including their courts — are up to. Governments like to be able to, and in some situations need to be able to, gather information about what “the governed” are up to. In recent times the fear generated by an unknown enemy with unknown resources rightly leaves state authorities anxious to preserve whatever advantage they might enjoy in combating terror. at the end of the day the test of a democratic legal system is not whether it permits secret proceedings, or gives the state the power to discover private information: obviously for any sovereign authority to function in a meaningful way it must be able do these things, at least some of the time. Our focus should be on the procedures in place to require the justification of these two departures from the (unattainable) democratic ideal of the perfectly unintrusive, transparent state I tend to the view that the Supreme Court of Canada has, by and large, struck an appropriate balance in matters related to the flow of information between and about state and individual. The judgments in Tessling and Mann are consistent with the Court’s previous jurisprudence and continue to approach issues of privacy and search in a principled, responsible manner. Section 8 guarantees only a reasonable expectation of privacy, a standard which requires an internal balancing of the state’s interest in the prompt and expeditious investigation of crime against the democratic ideal of an unintrusive government. Too broad a reading of reasonable expectation of privacy runs the risk of creating excessive and unnecessary hurdles to investigations without any significantly increasing the scope of democratically meaningful privacy