Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates

BACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics. OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method. METHODS: One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM. RESULTS: The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days. CONCLUSION: TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings

Sensitivity Analysis of a Comprehensive Model for a Miniature-Scale Linear Compressor for Electronics Cooling

A comprehensive model of a linear compressor for electronics cooling was previously presented by Bradshaw et al. (2011). The current study expands upon this work by first developing methods for predicting the resonant frequency of a linear compressor and for controlling its piston stroke. Key parameters governing compressor performance – leakage gap, eccentricity, and piston geometry – are explored using a sensitivity analysis. It is demonstrated that for optimum performance, the leakage gap and frictional parameters should be minimized. In addition, the ratio of piston stroke to diameter should not exceed a value of one to minimize friction and leakage losses, but should be large enough to preclude the need for an oversized motor. An improved linear compressor design is proposed for an electronics cooling application, with a predicted cooling capacity of 200 W a cylindrical compressor package size of diameter 50.3 mm and length 102 mm