10 research outputs found
Neonatal Screening of Cystic Fibrosis in France: a socio-material configuration of biomedical diagnostics and therapeutics
The influence of external factors on bacteriophagesâreview
The ability of bacteriophages to survive under unfavorable conditions is highly diversified. We summarize the influence of different external physical and chemical factors, such as temperature, acidity, and ions, on phage persistence. The relationships between a phageâs morphology and its survival abilities suggested by some authors are also discussed. A better understanding of the complex problem of phage sensitivity to external factors may be useful not only for those interested in pharmaceutical and agricultural applications of bacteriophages, but also for others working with phages
Short Communication - Biolistic inoculation of cassava (Manihot esculenta Crantz) with South African cassava mosaic virus
Cassava mosaic disease (CMD) is undoubtedly the most widespread disease
in cassava (Manihot esculenta Crantz ) in sub-Saharan Africa.
While African cassava mosaic virus-Kenya (ACMV-KE), African cassava
mosaic virus-Uganda (ACMV-UG), African cassava mosaic virus-India
(ACMV-IC), East African cassava mosaic virus-Cameroon (EACMV-CM) and a
recombinant East African cassava mosaic virus-India/Cameroon
(EACMV-CM/IC) can be transmitted easily and repeatedly to Nicotiana
benthamiana plants, difficulty in mechanical transmission and
multiplication of EACMV-UG and South African cassava mosaic virus
(SACMV) in N. benthamiana appears to place them biologically in a
different group. In vitro grown 3-week-old cassava plants were
biolistically inoculated with SACMV DNA A and B dimers and infectivity
measured using a system based on visual assessment. We report for the
first time successful infection, induced by SACMV DNA A and B dimer, of
cassava cultivar TMS60444 using biolistic inoculation. Typical mosaic
symptoms started to show at 14 days post-inoculation (dpi) in infected
cassava plants, and SACMV replication was confirmed using PCR.
Potential applications of biolistic infection of SACMV are also
discussed