1,486 research outputs found
Poly(amido amine)s as Gene Delivery Vectors: Effects of Quaternary Nicotinamide Moieties in the Side Chains
To evaluate the effect of quaternary nicotinamide pendant groups on gene delivery properties, a series of poly(amido amine) (co)polymers were synthesized by Michael addition polymerization of N, N-cystaminebisacrylamide with variable ratios of 1-(4-aminobutyl)-3-carbamoylpyridinium (Nic-BuNH2), and tert-butyl-4-aminobutyl carbamate (BocNH-BuNH2), yielding poly(amido amine)s (NicX-NHBoc) with X=0, 10, 30, and 50 % of quaternary nicotinamide groups in the polymer side chains. Deprotection of the pendant Boc-NH groups afforded an analogous series of polymers (NicX-NH2) with higher charge density (due to the presence of protonated primary amino groups in the side chains) and subsequent acetylation yielded a series of polymers (NicX-NHAc) of lower hydrophobicity than the Boc-protected polymers. The polymers with the Boc-protected or the acetylated amino groups showed high buffer capacity in the range pH 5.1-7.4, which is a property that can contribute to endosomal escape of polyplexes. The presence of quaternary nicotinamide groups has distinct beneficial effects on the gene vector properties of these polymers. The polymers containing 30 % of quaternary nicotinamide groups in their side chains condense DNA into small, nanosized particles (200 nm) with positive surface charge (+15 mV). Fluorescence experiments using ethidium bromide as a competitor showed that the quaternary nicotinamide groups intercalate with DNA, contributing to a more intimate polymer-DNA binding and shielding. Polyplexes of nicotinamide-functionalized poly(amido amine)s NicX-NHBoc and NicX-NHAc, formed at 12/1 polymerDNA mass ratio, efficiently transfect COS-7 cells with efficacies up to four times higher than that of PEI (Exgen 500), and with essentially absence of cytotoxicity. NicX-NH2 polymers, possessing protonated primary amino groups in their side chains, have a higher cytotoxicity profile under these conditions, but at lower 3/1 polymer-DNA mass ratio also these polymers are capable of efficient transfection, while retaining full cell viability
Electron spin resonance on a 2-dimensional electron gas in a single AlAs quantum well
Direct electron spin resonance (ESR) on a high mobility two dimensional
electron gas in a single AlAs quantum well reveals an electronic -factor of
1.991 at 9.35 GHz and 1.989 at 34 GHz with a minimum linewidth of 7 Gauss. The
ESR amplitude and its temperature dependence suggest that the signal originates
from the effective magnetic field caused by the spin orbit-interaction and a
modulation of the electron wavevector caused by the microwave electric field.
This contrasts markedly to conventional ESR that detects through the microwave
magnetic field.Comment: 4 pages, 4 figure
Collaborative Project Across Three Hong Kong Universities: A Case Study in E-Commerce Education
This paper reports on the work undertaken by three tertiary institutions in Hong Kong to provide business students with the opportunity to experience a project-based teamwork game in learning e-commerce (EC). The teaching objective of this EC project is to develop the knowledge and skills of students, such as in the use of EC site-building tools, critical thinking, communication skills, teamwork, and entrepreneurship. This study examined student attitudes toward the learning in introductory e-commerce course via a project-based teamwork game in EC using a non-traditional teaching approach. The results ofan evaluation indicate that the project-based teamwork approach performed to expectations. Based on the feedback from students from the three tertiary institutions, the project was found to facilitate the teaching and learning of EC and to be interesting, exciting, innovative, and more worthwhile than traditional textbook-based learning
Random and block copolymers of bioreducible poly(amido amine)s with high- and low-basicity amino groups: study of DNA condensation and buffer capacity on gene transfection
Poly(amido amine) (SS-PAA) random and block copolymers having bioreducible disulfide bonds in the main chain and amino groups with distinctly different basicity in the side chain were designed and synthesized by Michael addition polymerization between N, N′-cystaminebisacrylamide (CBA) and two amine monomers, i.e., histamine (HIS) and 3-(dimethylamino)-1-propylamine (DMPA). Copolymers containing variable HIS/DMPA ratios show higher ability to bind DNA than p(CBA-HIS) homopolymer and condense DNA into the polyplexes with particle sizes (< 150 nm) that are smaller than polyplexes of p(CBA-HIS) (not, vert, similar 220 nm). The buffer capacities of the copolymers increase with increasing HIS/DMPA ratio. These copolymers are able to transfect COS-7 cells in vitro with efficiencies that increase with increasing HIS/DMPA ratio. The random and block copolymers at a HIS/DMPA ratio of 70/30 combines optimal DNA condensation capability and buffer capacity, thereby inducing higher transfection efficiency in the absence and presence of serum as compared to p(CBA-HIS) homopolymer. Moreover, random and block copolymers show a similar transfection capacity, but both have higher capacity than the physical mixtures of p(CBA-HIS) and p(CBA-DMPA) homopolymers. XTT assay reveals that the polyplexes of the SS-PAA copolymers have essentially low cytotoxicity when the highest transfection activity is observed
Tunneling Via Individual Electronic States in Ferromagnetic Nanoparticles
We measure electron tunneling via discrete energy levels in ferromagnetic
cobalt particles less than 4 nm in diameter, using non-magnetic electrodes. Due
to magnetic anisotropy, the energy of each tunneling resonance shifts as an
applied magnetic field rotates the particle's magnetic moment. We see both
spin-increasing and decreasing tunneling transitions, but we do not observe the
spin degeneracy at small magnetic fields seen previously in non-magnetic
materials. The tunneling spectrum is denser than predicted for independent
electrons, possibly due to spin-wave excitations.Comment: 4 pages, 4 figures. Improved by comments from referees, to appear in
Phys. Rev. Let
Semiclassical theory of electron drag in strong magnetic fields
We present a semiclassical theory for electron drag between two parallel
two-dimensional electron systems in a strong magnetic field, which provides a
transparent picture of the most salient qualitative features of anomalous drag
phenomena observed in recent experiments, especially the striking sign reversal
of drag at mismatched densities. The sign of the drag is determined by the
curvature of the effective dispersion relation obeyed by the drift motion of
the electrons in a smooth disorder potential. Localization plays a role in
explaining activated low temperature behavior, but is not crucial for anomalous
drag per se.Comment: 10 page
Genetic counseling of patients with ovarian carcinoma:acceptance, timing, and psychological wellbeing
The new Dutch guidelines on hereditary and familial ovarian carcinoma recommend genetic testing of all patients with epithelial ovarian cancer (EOC). With this study, we aimed to obtain insight into (1) the acceptance and timing of the offer of genetic counseling in women with EOC, (2) reasons for accepting or declining genetic counseling, and (3) psychological differences between women who did and did not have genetic counseling. A multicenter questionnaire survey was performed in patients with EOC in four Dutch oncology centers. The questionnaire addressed whether, how, and when genetic counseling was offered, women's arguments to accept or decline genetic counseling, and included the Cancer Worry Scale (CWS) and the Hospital Anxiety and Depression Scale (HADS). A total of 67 women completed the questionnaire, of which 43 had genetic counseling. Despite a wide variability in the timing of the offer of genetic counseling, 89% of the women were satisfied with the timing. No significant differences were found between the CWS and HADS scores for the timing of the offer of genetic counseling and whether or not women had genetic counseling. Taking the small sample size into account, the results tentatively suggest that genetic counseling may have limited impact on the psychosocial wellbeing of women with EOC. Therefore, we assume that implementation of the new guidelines offering genetic counseling to all patients with EOC will not cause considerable additional burden to these patients
The Influence of Health Care Professional Characteristics on Pain Management Decisions
Objective
Evidence suggests that patient characteristics such as sex, race, and age influence the pain management decisions of health care providers. Although this signifies that patient demographics may be important determinants of health care decisions, pain-related care also may be impacted by the personal characteristics of the health care practitioner. However, the extent to which health care provider characteristics affect pain management decisions is unclear, underscoring the need for further research in this area.
Methods
A total of 154 health care providers (77 physicians, 77 dentists) viewed video vignettes of virtual human (VH) patients varying in sex, race, and age. Practitioners provided computerized ratings of VH patients’ pain intensity and unpleasantness, and also reported their willingness to prescribe non-opioid and opioid analgesics for each patient. Practitioner sex, race, age, and duration of professional experience were included as predictors to determine their impact on pain management decisions.
Results
When assessing and treating pain, practitioner sex, race, age, and duration of experience were all significantly associated with pain management decisions. Further, the role of these characteristics differed across VH patient sex, race, and age.
Conclusions
These findings suggest that pain assessment and treatment decisions may be impacted by the health care providers’ demographic characteristics, effects which may contribute to pain management disparities. Future research is warranted to determine whether findings replicate in other health care disciplines and medical conditions, and identify other practitioner characteristics (e.g., culture) that may affect pain management decisions
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