MOESM4 of Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages

Additional file 4. Microscopic visualization of the parietal cells (A), D-cells (B) and G-cells (C–D) in the porcine stomach using immunohistochemistry. (A) H+/K+ ATPase staining of the fundic gland zone of a H. suis-positive adult sow, showing parietal cells (brown). No clear parietal cell loss was detected. Original magnification ×100. (B) Somatostatin staining of the pyloric gland zone of a H. suis-positive adult sow, showing D-cells (brown). Original magnification ×200. (C) Gastrin staining of the pyloric gland zone of a H. suis-positive adult sow, showing G-cells (brown). Original magnification ×200. (D) Gastrin staining of the pyloric gland zone of a H. suis-negative sow, showing G-cells (brown). Original magnification ×200. The number of G-cells in the H. suis-negative sow (D) is lower than observed in the H. suis-positive sow (C)

MOESM9 of Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages

Additional file 9. Correlations of altered markers for gastric acid secretion with the number of H. suis bacteria and with the altered markers for inflammation in H. suis -infected pigs of different age groups. r = Pearson correlation coefficient, calculated using SPSS Statistics 24®. A r-value close to 1 indicates a strong, positive correlation, whereas a r-value of −1 indicates a strong, negative correlation. P-values lower than 0.05 are considered to be significant./= no clear correlation, yes = correlation with H. suis colonization rate (see Additional files 6, 8 for the r and p-values)

MOESM8 of Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages

Additional file 8. Overview of important correlations between markers for gastric acid secretion and the number of H. suis bacteria in pigs of different ages. r = Pearson correlation coefficient, calculated using SPSS Statistics 24Ž. A r-value close to 1 indicates a strong, positive correlation, whereas a r-value of -1 indicates a strong, negative correlation. P-values lower than 0.05 are considered to be significant

MOESM3 of Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages

Additional file 3. General overview of the average scores of infiltration with inflammatory cells and lymphoid follicle formation in the fundic and pyloric gland zone of pigs of different ages. Gastritis was scored based on infiltration with inflammatory cells/lymphoid follicle formation, with score 0 = absence of infiltration/absence of lymphoid aggregates, 1 = mild infiltration/small number of lymphoid aggregates (n < 5), 2 = moderate infiltration/large number of lymphoid aggregates (n > 5) or presence of 1 organized lymphoid follicle, 3 = marked infiltration/at least 2 organized lymphoid follicles, n = total number of investigated pigs’ stomachs per age group. The data are shown as the average of the administered scores with standard deviation

MOESM6 of Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages

Additional file 6. Overview of important correlations between markers for inflammation and the number of H. suis bacteria in pigs of different ages. r = Pearson correlation coefficient, calculated using SPSS Statistics 24®. A r-value close to 1 indicates a strong, positive correlation, whereas a r-value of −1 indicates a strong, negative correlation. A p-value lower than 0.05 is considered to be significant