Genetic Studies of Sulfadiazine-resistant and Methionine-requiring \u3cem\u3eNeisseria\u3c/em\u3e Isolated From Clinical Material

Deoxyribonucleate (DNA) preparations were extracted from Neisseria meningitidis (four isolates from spinal fluid and blood) and N. gonorrhoeae strains, all of which were resistant to sulfadiazine upon primary isolation. These DNA preparations, together with others from in vitro mutants of N. meningitidis and N. perflava, were examined in transformation tests by using as recipient a drug-susceptible strain of N. meningitidis (Ne 15 Sul-s Met+) which was able to grow in a methionine-free defined medium. The sulfadiazine resistance typical of each donor was introduced into the uniform constitution of this recipient. Production of p-aminobenzoic acid was not significantly altered thereby. Transformants elicited by DNA from the N. meningitidis clinical isolates were resistant to at least 200 μg of sulfadiazine/ml, and did not show a requirement for methionine (Sul-r Met+). DNA from six strains of N. gonorrhoeae, which were isolated during the period of therapeutic use of sulfonamides, conveyed lower degrees of resistance and, invariably, a concurrent methionine requirement (Sul-r/Met−). The requirement of these transformants, and that of in vitro mutants selected on sulfadiazine-agar, was satisfied by methionine, but not by vitamin B12, homocysteine, cystathionine, homoserine, or cysteine. Sul-r Met+ and Sul-r/Met− loci could coexist in the same genome, but were segregated during transformation. On the other hand, the dual Sul-r/Met− properties were not separated by recombination, but were eliminated together. DNA from various Sul-r/Met− clones tested against recipients having nonidentical Sul-r/Met− mutant sites yielded Sul-s Met+ transformants. The met locus involved is genetically complex, and will be a valuable tool for studies of genetic fine structure of members of Neisseria, and of genetic homology between species

Effective vanishing order of the Levi determinant

On a smooth domain in complex n space of finite D'Angelo q-type at a point, an effective upper bound for the vanishing order of the Levi determinant \text{coeff}\{\partial r \wedge \dbar r \wedge (\partial \dbar r)^{n-q}\} at that point is given in terms of the D'Angelo q-type, the dimension of the space n, and q itself. The argument uses Catlin's notion of a boundary system as well as techniques pioneered by John D'Angelo.Comment: 22 pages; typos in example from p.20 fixed in the second versio

Quaking Aspen in the Residential-Wildland Interface: Elk Herbivory Hinders Forest Conservation

Quaking aspen (Populus tremuloides Michx.) forests are experiencing numerous impediments across North America. In the West, recent drought, fire suppression, insects, diseases, climate trends, inappropriate management, and ungulate herbivory are impacting these high biodiversity forests. Additionally, ecological tension zones are sometimes created where the above factors intermingle with jurisdictional boundaries. The public-private land interface may result in stress to natural areas where game species find refuge and plentiful forage at the expense of ecosystem function. We examined putative herbivore impacts to aspen forests at Wolf Creek Ranch (WCR), a large residential landscape in northern Utah. Forty-three ha-1 monitoring plots were established to measure a range of attributes summarizing location description, tree and vegetation condition, and herbivore presence. Additionally, we tested the ability of a stand-level visual rating system to represent more detailed field measures. Elk (Cervus elaphus L.) herbivory is currently having a strong effect on aspen in the study area, reducing many locations to single-layer aspen forests dominated by aging canopy trees. Regeneration (\u3c 2 m stems) is experiencing moderate-to-high browse and recruitment (2 - 6 m stems) are below replacement levels on approximately half of WCR\u27s aspen forests. The condition rating system represented significant trends in forest cover, canopy height, stand aspect, regeneration, recruitment, and tree mortality. Ordination of all plot and forest data found a strong negative relationship between elk presence and recruitment success. We make recommendations for addressing difficult herbivore-aspen interactions where publicly managed wildlife a present barriers to conservation of privately owned forest reserves

Entire curves avoiding given sets in C^n

Let $F\subset\Bbb C^n$ be a proper closed subset of $\Bbb C^n$ and $A\subset\Bbb C^n\setminus F$ at most countable ($n\geq 2$). We give conditions of $F$ and $A$, under which there exists a holomorphic immersion (or a proper holomorphic embedding) $\phi:\Bbb C\to\Bbb C^n$ with $A\subset\phi(\Bbb C)\subset\Bbb C^n\setminus F$.Comment: 10 page