One-step transformation of the dimorphic yeast Yarrowia lipolytica

International audienceAn efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated

One-step transformation of the dimorphic yeast Yarrowia lipolytica

International audienceAn efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated

LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast

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Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue

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LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast

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Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue

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Isolation and cloning of the Yarrowia lipolytica SEC65 gene, a component of the yeast signal recognition particle displaying homology with the human SRP19 gene

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High speed rail service and socio economic transformations in local areas, a review

Many High-Speed Rail (HSR) projects exist in Europe and elsewhere in the world and generate many expectations of stakeholders in local economic areas. But their effects are controversial. There is a gap between academic literature (AL) and non-academic literature (NAL). The aim of the paper is to present a review of both kinds of literature because the effects of HSR are far from being a closed debate in AL. We highlight the relative proximity between AL and the stakeholders' expectations in local economic areas. We emphasize however the more general characteristics of the effects mentioned by NAL which appear to be a first explanation of the continuity of this controversy. We also show that AL identifies some extremely diverse and often more precise conditions for these effects, which are only partially presented in NAL. The area stakeholders omit the specificities of local areas and the largely contextualized effects and conditions. Another explanation could be that although AL puts the effects into perspective, it does not really question the effects. It supposes that, in specific cases, there will be effects if the conditions are present. The myth according to which automatic effects of High-Speed Rail on local economic development will occur can then continue to exist. But there is a risk to generalize conclusions that are linked to particular cases in terms of the economic situation, the geographical location, the quality of the service, attendance, specific resources and the actors' strategies, etc

Isolation and cloning of the Yarrowia lipolytica SEC65 gene, a component of the yeast signal recognition particle displaying homology with the human SRP19 gene

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