6 research outputs found
COLD SWIMMING STRESS - EFFECTS ON SERUM-LIPIDS, LIPOPROTEINS AND LCAT ACTIVITY IN MALE AND FEMALE RATS
Effects of consistent cold swimming stress on lipid and lipoprotein
metabolism parameters were studied using male and female rats over a
period of 60 and 20 days respectively. At the end of treatment serum
total cholesterol, high density lipoproteins (HDL-C) and
lecithin:cholesterol acetyltransferase (LCAT) activity declined in both
male and female rats. TC/HDL-C ratio declined in 20 days in females,
while in males it did not change. Free fatty acids increased, while
triglycerides remained unchanged in both sexes. %Lipoprotein
distribution in male animals did not show any phenotype alteration
except in the group of 40 days where %VLDL declined and %LDL-C
increased. Body weights did not change, except in males in 60 days.
Consistent cold swimming stress by lowering HDL-C and LCAT activity
seems to influence lipoprotein metabolism
Changes in serum levels of quinolones in rats under the influence of experimental trauma
Administration of antibiotics is considered an important factor during, or after, operational procedures in the maxillofacial area, in order to avoid post-surgical complications. In the present study, the levels of quinolones in serum and tissues such as the parotid gland, the tongue and the bone of the jaws were estimated during traumatic injury in the oral cavity. For this purpose, two groups (A and B) of Wistar rats, consisting of 35 animals each were used: Group A (control) and group B (experimental) were divided in five subgroups (A1, A2, A3, A4, A5 and B1, B2, B3, B4, B5). In the experimental group, model traumatic injury was performed through the Whole length of the cheek. Subjects received orally ciprofloxacin, pefloxacin, norfloxacin, ofloxacin and cinoxacin: The concentration of quinolones in serum and in most of the tissues was significantly higher in the experimental groups than in controls. In addition, the FFA levels and the weight of adrenals (as indicators of stress) were higher in the trauma groups: Stress seemed to affect many pathophysiological mechanisms which are responsible for the alterations observed
Modifications of clonidine binding to rabbit liver protein under the influence of non-steroid-ant-inflammatory drugs in vitro
This study was designed to investigate the binding of clonidine to liver
protein as well as the possible interactions with non-steroid
anti-inflammatory drugs (NSAIDs) during the binding process in the
rabbit.
The binding of clonidine to slices (S) and homogenized slices (H) was
estimated by a radioisotopic method following incubation with a mixture
of cold and H-3-labelled clonidine in Ringer solution at 37degreesC for
360 min.
The binding of clonidine was assessed in the absence and presence of the
following NSAIDs: flurbiprofen, ketoprofen, ibuprofen and
acetylsalicylic acid.
The results showed that the percentage of clonidine binding did not
differ between intact and homogenized slices.
The addition of all NSAIDs but ibuprofen, significantly decreased the
protein binding of clonidine both in intact and homogenized liver
slices.
This finding could be attributed to the different affinity of ibuprofen
for liver protein compared to the remaining NSAID’s which may arise from
a number of chemical properties including its dual Pka values
How harmless is FFA enhancement?
Standard heparin as well as low molecular weight heparin (LMWH) increase
lipid levels in serum. It has been reported that a diet rich in long
chain saturated fatty acids can enhance the susceptibility to
experimental thrombosis. The mechanism by which serum fatty acids may
provoke thrombosis is not clear. It is possible that the fatty acids
change the properties of the cell membrane and thereby modify the
response of platelets to aggregating agents.
Heparin and its LMW fractions, by mobilising lipoprotein lipase that
hydrolyses serum triglycerides (TG), cause the serum TG to increase, a
well known ‘clearing effect’ of heparin in turbid lipemic plasma. This
effect may have no significance when it lasts for a short time; however,
a long-lasting heparin effect on TG serum levels may have important
consequences.
The purpose of this study was to examine the time span of the action of
heparin and its fractions and to investigate variations in the
concentration of digoxin, which is a compound with narrow therapeutic
width.
The investigated substances after 2 days administration, provoked serum
concentration increases of free fatty acids (FFA), TG and HDL-C. Seven
days after stopping drug administration, FFA and HDL-C levels remained
high, while triglycerides declined. Serum total cholesterol remained
unchanged throughout. Digoxin levels increased non-significantly after
heparin administration and during swimming stress, while a lipid diet
caused a serum digoxin concentration increase