In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

Biosynthesis of silver nanoparticles using Planomicrobium sp. and to explore the antibacterial activity against food borne pathogenic bacteria Bacillus subtilis, (3053) Klebsiella planticola (2727) Klebsiella pneumoniae (MAA) Serratia nematodiphila (CAA) and Escherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL of Planomicrobium sp. culture supernatant. The bioreduction of pure AgNO3 was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety

SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM MARINE BROWN SEAWEED AND ITS ANTIFUNGAL EFFICIENCY AGAINST CLINICAL FUNGAL PATHOGENS

Objectives: The aim of this study is to synthesize silver nanoparticles using the algal extract of Padina tetrastromatica and evaluate its antifungal activity against pathogenic fungus isolated from clinical samples.Results: Formation of brown color at 15 minutes indicates the production of silver nanoparticles by the extract of brown algae P. tetrastromatica. Surface plasmon resonance band was centered at 440 nm which was observed by UV-vis spectrophotometer. SEM image revealed spherical and cubical nanoparticles with high agglomeration, and energy-dispersive X-ray illustrates elemental components of silver formed at 3 keV. TEM shows spherical, truncated, and ellipsoidal nanoparticles and also it evidences the algae compounds that are capped with nanoparticles. SAED pattern proved four diffraction face-centered cubic rings at (111), (200), (220), and (311) which indicates the crystalline nature of nanoparticles. Silver nanoparticles show high inhibition activity against Fusarium sp, Aspergillus niger, Candida albicans, Aspergillus fumigatus, and Aspergillus flavus at different concentrations. P. tetrastromatica-mediated synthesis of silver nanoparticles shows rapid and eco-friendly silver ion reduction process.Methods: Dried algal biomass was used to prepare the pure algal extract and added with 1 mM AgNO , and the color change was noted and recorded by ultraviolet (UV)-vis spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Crystalline structure was analyzed by SAED pattern. Antifungal activity was performed by agar well diffusion method against various pathogenic fungi.Conclusion: Therefore, this present study elucidates that algae-mediated synthesized silver nanoparticles have antifungal activity against pathogenic fungi, so it can be developed as a novel medicine for human welfare in biomedical applications in the near future.Keywords: Padina tetrastromatica, Silver nanoparticles, Transmission electron microscopy, Antifungal activity, Green synthesis.Â

Generic Feature Selection Methodology to Named Entity Detection from Indian and European Languages

This paper describes the development of language and domain independent Named Entity Recognition (NER) system which can identify named entities from any given dataset irrespective of the language and domain. The main novelty of the present work is the generic feature selection methodology which has been applied to 7 Indian languages and 5 European languages. The generic feature selection methodology was done in two ways; first using frequency based approach; secondly k-means++ clustering algorithm was used to validate the patterns obtained in the frequency based approach. The dataset used for the experiments belongs to different genre. To the best of our knowledge we are the first to work on the development of cross-lingual Named Entity (NE) system with 12 languages belongs to different language families. We have done the 10-fold cross validation and the system output has been analyzed for all the languages and causes of error cases was discussed in the error analysis section. The performance of our system is also compared with the existing systems

Biosynthesis and Antimicrobial Activity of Semiconductor Nanoparticles against Oral Pathogens

Dental care is an essential phenomenon in human health. Oral pathogens can cause severe break which may show the way to serious issues in human disease like blood circulation and coronary disease. In the current study, we demonstrated the synthesis and antimicrobial activity of cadmium sulphide and zinc sulphide nanoparticles against oral pathogens. The process for the synthesis of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles is fast, novel, and ecofriendly. Formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was confirmed by surface plasmon spectra using UV-Vis spectrophotometer. The morphology of crystalline phase of nanoparticles was determined from transmission electron microscopy (TEM) and X-ray diffraction (XRD) spectra. The average size of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was in the range of 10 nm to 25 nm and 65 nm, respectively, and the observed morphology was spherical. The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution. The antimicrobial activity was assessed against oral pathogens such as Streptococcus sp. Staphylococcus sp. Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity

Optimized Synthesis of Gold Nanoparticles using Green Chemical Process and its Invitro Anticancer Activity Against HepG2 and A549 Cell Lines

International audienceIn the present study, the biosynthesis of gold nanoparticles (AuNPs) was achieved by using marine brown seaweed Padina tetrastromatica (PT) as a reducing and capping agent. The optimized Au Nps were performed by changing the concentration of algae extract, pH and temperature was analyzed by UV-vis spectrophotometer. The synthesized Au NPs were characterized byXRD, FTIR, SEM, TEM, EDX and SAED. The X-ray diffraction showed the Au NPs which can state by the presence of peaks at (1 1 1), (2 0 0), (2 2 0) and (2 2 2). The FTIR result clearly showed that the extracts containing-OH as a functional group (sugar molecules) act in capping the nanoparticles synthesis. SEM images revealed that all particles were spherical in shape. TEM image confirms the spherical in shape with an average size ranges from 8-10 nm. Synthesized Au NPs were evaluate for the in vitro cytotoxic activity of human liver cancer (HepG2) and lung cancer (A549) cell line at the different concentrations compared with standard drug cyclophosphamide