“Imagining others more complexly”: Celebrity and the ideology of fame among YouTube’s ‘Nerdfighteria’

YouTube has witnessed the growth of a celebrity culture of its own. This article explores the celebritification of online video-bloggers in relation to their own discursive community. Focusing on the VlogBrothers (John and Hank Green) and their community ‘Nerdfighters’, this article demonstrates how their philosophy of “Imagining Others More Complexly” (IOMC) is used to debate ‘celebrity’ and its legitimacy. Their vision of celebrity is egalitarian and democratic, rooted in Western culture’s ‘expressive turn’ (Taylor, 1989). It views each person as a unique individual and others as equal, legitimate subjects. Situating this discursive formation within the culture of web 2.0’s neo-liberal developments, the article seeks to explore the contradictory origins and uses to which IOMC is directed. While utilised to promote a vision of vloggers beyond the gaze and mystification of ‘celebrity’, it is also drawn upon by celebrities to manage and control perception and preserve good public opinion amongst the community. The article concludes with a discussion of how this philosophy may conceive of ‘celebrity’ as a model of expressive individualism beyond its commercial uses. ‘IOMC’ may be considered a state of ‘selfhood’ which allows each person equal space to consummate a unique vision of themselves

Mutations in the gene encoding ε-sarcoglycan cause myoclonus-dystonia syndrome.

The dystonias are a common clinically and genetically heterogeneous group of movement disorders. More than ten loci for inherited forms of dystonia have been mapped, but only three mutated genes have been identified so far. These are DYT1, encoding torsin A and mutant in the early-onset generalized form, GCH1 (formerly known as DYT5), encoding GTP-cyclohydrolase I and mutant in dominant dopa-responsive dystonia, and TH, encoding tyrosine hydroxylase and mutant in the recessive form of the disease. Myoclonus-dystonia syndrome (MDS; DYT11) is an autosomal dominant disorder characterized by bilateral, alcohol-sensitive myoclonic jerks involving mainly the arms and axial muscles. Dystonia, usually torticollis and/or writer's cramp, occurs in most but not all affected patients and may occasionally be the only symptom of the disease. In addition, patients often show prominent psychiatric abnormalities, including panic attacks and obsessive-compulsive behavior. In most MDS families, the disease is linked to a locus on chromosome 7q21 (refs. 11-13). Using a positional cloning approach, we have identified five different heterozygous loss-of-function mutations in the gene for epsilon-sarcoglycan (SGCE), which we mapped to a refined critical region of about 3.2 Mb. SGCE is expressed in all brain regions examined. Pedigree analysis shows a marked difference in penetrance depending on the parental origin of the disease allele. This is indicative of a maternal imprinting mechanism, which has been demonstrated in the mouse epsilon-sarcoglycan gene

Exploring the Biocatalytic Potential of a Self-Sufficient Cytochr ome P450 from Thermothelomyces thermophila

Among nature's arsenal of oxidative enzymes, cytochrome P450s (CYPs) catalyze the most challenging reactions, the hydroxylations of non‐activated C−H bonds. Human CYPs are studied in drug development due to their physiological role at the forefront of metabolic detoxification, but their challenging handling makes them unsuitable for application. CYPs have a great potential for biocatalysis, but often lack appropriate features such as high and soluble expression, self‐sufficient internal electron transport, high stability, and an engineerable substrate scope. We have probed these characteristics for a recently described CYP that originates from the thermophilic fungus Thermothelomyces thermophila (CYP505A30), a homolog of the well‐known P450‐BM3 from Bacillus megaterium. CYP505A30 is a natural monooxygenase‐reductase fusion, is well expressed, and moderately tolerant towards temperature and solvent exposure. Although overall comparable, we found the stability of the enzyme's domains to be inverse to P450‐BM3, with a more stable reductase compared to the heme domain. After analysis of a homology model, we created mutants of the enzyme based on literature data for P450‐BM3. We then probed the enzyme variants in bioconversions using a panel of active pharmaceutical ingredients, and activities were detected for a number of structurally diverse compounds. Ibuprofen was biooxidized in a preparative scale whole cell bioconversion to 1‐, 2‐ and 3‐hydroxyibuprofen