27 research outputs found
Regenerative ability of gastrocnemius muscle under diabetic condition with special reference to SDH & m-ATPase
567-574The present study analyzes the
regeneration of skeletal muscle in streptozotocin (STZ) induced diabetic rats. Two
weeks later the gastrocnemius muscle from diabetic rats were transplanted into diabetic
and normal host to initiate regeneration and the normal gastrocnemius muscle was
transplanted in normal and diabetic hosts for comparison. The regenerates were
analyzed after 15 and 30 days of transplantation for histochemical (with respect
to SDH and m-ATPase) and 7, 14, 21 and 28 days after transplantation for biochemical
studies (with respect to SDH and m-ATPase). Least enzymatic activity and
the poorest regenerative ability in case
of normal muscle in diabetic host (NM-DbH) and comparatively higher enzymatic activity
and better regenerative ability in diabetic muscle in normal host (DbM-NH) was
observed. The result of this study strongly supports that the normal host
environment is crucial for the muscle recovery
Effect of thyroidectomy on fast and slow muscle fibres of rat gastrocnemius muscle
575-579A combined histochemical, biochemical and
electrophoretic study with respect to the enzymes succnic dehydrogenase(SDH),myofibrillar
adenosine triphosphatase (m-ATPase), lactate dehydrogenase (LDH) isozymes and myosin
light chains was carried out to investigate the response of rat gastrocnemius muscle
(medial head). Twelve weeks after thyroidectomy,
the results indicated a shift from fast
to slow type pattern of LDH isozymes, fibre type transformation from Type II to
Type I and a decrease in SDH and m-ATPase activity. The results suggest, possible
thyroidal involvement in determining the phenotypic properties of skeletal muscle