Vibrational Signature Of Charge Solvation Vs Salt Bridge Isomers Of Sodiated Amino Acids In The Gas Phase

International audienceThe vibrational spectra of the gaseous sodium complexes of glycine (Gly-Na+) and proline (Pro-Na+) have been recorded in the spectral range 1150-2000 cm-1. The complexes were formed by matrix-assisted laser desorption-ionisation, introduced in the cell of a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, and their infrared absorption spectra were recorded using photons of variable energy emitted by a free electron laser. Photon absorption was probed by the diminished intensity of the parent ion, due to its infrared induced dissociation into bare sodium cation and the free amino acid, and the appearance of Na+. The observed absorption bands are assigned using ab initio computations of the IR spectra of the lowest energy isomers in each case. They provide the first experimental evidence that the salt bridge isomer is formed in the case of Pro-Na+. In contrast, charge solvation by chelation of Na+ between nitrogen and the carbonyl oxygen seems to be most favorable for Gly-Na+, but a mixture of isomers cannot be ruled out in this case

Preincisional versus postincisional administration of parecoxib in colorectal surgery: Effect on postoperative pain control and cytokine response. A randomized clinical trial

Background: Preincisional pain management aims at reducing pain and inflammatory response. We investigated whether preincisional parecoxib administration reduces pain, opioid requirements, and cytokine production after surgery for colonic cancer. Methods: Forty one patients whose American Society of Anesthesiologists (ASA) status was I-II and who were scheduled for colorectal cancer surgery were randomly divided in two groups according to the timing of parecoxib administration: Group PRE (preincisional) received parecoxib 40 mg intravenously 30 min before skin incision and group POST (postincisional) received the same dose 30 min after skin incision. Postoperative analgesia involved the administration of patient-controlled analgesia (PCA) morphine to all patients. We recorded verbal rating scale (VRS) scores and morphine consumption at 1, 6, 18, and 24 h after surgery and blood levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-α) 30 min before skin incision, at peritoneal closure, and 24 h postoperatively. Results: The VRS scores were similar between groups. Although morphine consumption was significantly lower in group PRE at 6, 18 and 24 h postoperatively (p = 0.044, p = 0.02, p < 0.001, respectively) morphine-related adverse effects did not differ between the two groups. The serum IL-6 was significantly (p = 0.042) elevated from the baseline value 24 h postoperatively in group POST. Conclusions: Preincisional parecoxib administration compared to postincisional administration reduced postoperative morphine consumption, but without affecting morphine-related adverse effects and attenuated IL-6 production 24 h after surgery for colorectal cancer. © 2010 Société Internationale de Chirurgie

Peritoneal dialysis as a therapeutic solution in elderly patients with cardiorenal syndrome and heart failure: A case-series report

Background: The aim of this work was to evaluate the impact of peritoneal dialysis (PD) on venous congestion, right ventricular function, pulmonary artery systolic pressure (PASP), and clinical functional status in elderly patients with cardiorenal syndrome (CRS) and chronic heart failure (HF). Methods: A case series of 21 (17 males, age 70 ± 11 years) consecutive patients with HF along with diuretic resistance and right ventricular dysfunction (median renal failure duration 60 months, range 13-287 months, mean ejection fraction 36 ± 11%) having been engaged in PD; 76% of the patients were under automated peritoneal dialysis (APD), whereas the rest were under continuous ambulatory PD (CAPD). Patients’ PASP and central venous pressure (CVP) – through compression sonography – and body weight were evaluated before initiating the PD program and at 6 and 12 months. Results: During the follow-up period, the mortality rate was 8 deaths out of 21 patients (38%) A significant reduction by 29.9% in PASP levels (p = 0.013) and by 42% in CVP levels (p < 0.001), and in right ventricular function assessed by tricuspid annulus tissue Doppler velocity (p = 0.04) was observed, whereas patients’ weight increased by 3.7% (p = 0.001). New York Heart Association class improved in 12 patients, whereas in the remaining patients, it remained constant (p = 0.046). In 8 patients, complications were reported (mainly presence of Staphylococcus aureus). In conclusion, PD seems to confer a substantial benefit in clinical status, which is in line with improvement in venous congestion and right ventricular systolic pressure among elderly patients with HF along with CRS. © 2019 Hellenic Society of Cardiolog