An incubation study on the stability and biological effects of pyrogenic and hydrothermal biochar in two soils

The success and feasibility of CO2-sequestration through incorporation of biochar into soils depends strongly on the long-term biochar stability and on the improvements of physical and microbial soil properties. In this study, the stability of two maize-derived biochars (from pyrolysis and hydrothermal carbonization) and of a compost-biochar mixture and their effects on microbial biomass and enzyme activity were determined in two soils during a 57-day incubation. Soil samples amended with biochar increased soil organic carbon (SOC) content by 20 or 40%. Samples amended with hydrothermal biochar showed the largest respiration rates and the largest increase in microbial and enzymatic activity compared with the untreated controls. Carbon and 13C mass balances showed that between 13 and 16% of the added hydrochar was mineralized within 8 weeks. In the arable soil, hydrochar additions greatly stimulated the degradation of SOC, thus inducing positive priming effects. The mineralization of pyrogenic biochar (pyrochar and a pyrochar-compost mixture) was significantly less (1.4–3%) and comparable to the SOC mineralization in the control soils

Inter-individual variability and genetic influences on cytokine responses to bacteria and fungi

Little is known about the inter-individual variation of cytokine responses to different pathogens in healthy individuals. To systematically describe cytokine responses elicited by distinct pathogens and to determine the effect of genetic variation on cytokine production, we profiled cytokines produced by peripheral blood mononuclear cells from 197 individuals of European origin from the 200 Functional Genomics (200FG) cohort in the Human Functional Genomics Project (http://www.humanfunctionalgenomics.org), obtained over three different years. We compared bacteria- and fungi-induced cytokine profiles and found that most cytokine responses were organized around a physiological response to specific pathogens, rather than around a particular immune pathway or cytokine. We then correlated genome-wide single-nucleotide polymorphism (SNP) genotypes with cytokine abundance and identified six cytokine quantitative trait loci (QTLs). Among them, a cytokine QTL at the NAA35-GOLM1 locus markedly modulated interleukin (IL)-6 production in response to multiple pathogens and was associated with susceptibility to candidemia. Furthermore, the cytokine QTLs that we identified were enriched among SNPs previously associated with infectious diseases and heart diseases. These data reveal and begin to explain the variability in cytokine production by human immune cells in response to pathogens

Microtubule-Dependent mRNA Transport in Fungi▿

The localization and local translation of mRNAs constitute an important mechanism to promote the correct subcellular targeting of proteins. mRNA localization is mediated by the active transport of mRNPs, large assemblies consisting of mRNAs and associated factors such as RNA-binding proteins. Molecular motors move mRNPs along the actin or microtubule cytoskeleton for short-distance or long-distance trafficking, respectively. In filamentous fungi, microtubule-based long-distance transport of vesicles, which are involved in membrane and cell wall expansion, supports efficient hyphal growth. Recently, we discovered that the microtubule-mediated transport of mRNAs is essential for the fast polar growth of infectious filaments in the corn pathogen Ustilago maydis. Combining in vivo UV cross-linking and RNA live imaging revealed that the RNA-binding protein Rrm4, which constitutes an integral part of the mRNP transport machinery, mediates the transport of distinct mRNAs encoding polarity factors, protein synthesis factors, and mitochondrial proteins. Moreover, our results indicate that microtubule-dependent mRNA transport is evolutionarily conserved from fungi to higher eukaryotes. This raises the exciting possibility of U. maydis as a model system to uncover basic concepts of long-distance mRNA transport