Information technologies of enterprises and the problems of its employment in business development

The modern business enterprises, including agricultural enterprises, operates in dynamic knowledge environment, and its activity products and processes are more orientied to knowledge. Each business includes the elements of two types: physical elements (buildings, equipment, people) and virtual elements (information, various rules etc.). Receiving of qualitative information product is related with maturity of enterprises’ information technologies (IT). Practical activity and its results of IT subdivisions in Lithuania shows, that about 80% of enterprises depends to the first level of enterprises maturity. The activity of IT subdivisions is not clearly reglamented, work is chaotic, and they don’t have any permanent purposes, i.e. IT have only auxiliary role. Enterprises, which pretends to higher level of IT maturity, must understand the essential thing, that except investments to a newest technologies and high qualified specialists, it is very important to manage IT processes in enterprise and to concentrate people. Business enterprises with implementation of information technologies have choose the external IT companies for consulting and for IT maintanenceVytauto Didžiojo universitetasŽemės ūkio akademij

Expression of the connexin 43 gene is increased in the kidneys and the lungs of rats injected with bacterial lipopolysaccharide

At the molecular level, the inflammatory response is characterized by changes in gene expression of various organ systems. One gene by which expression has been observed to be altered in the liver during inflammation is connexin (Gx) 32. Ox genes encode the polypeptide subunits of the hemichannels that comprise gap junctions. In the present study, an increase in the expression of a different Cx gene, Cx43, was observed in the kidney and lung of rats injected with a sublethal dose (1 mg/kg) of bacterial lipolysaccharide (LPS). To elucidate the possible mechanism by which the Cx43 expression is increased during inflammation, the 5' flanking region of the gene was cloned and coupled to a reporter gene (human growth hormone). This construct was transfected into cells of renal origin (NRK), which express Cx43 constitutively. The Cx43 promoter activity was indeed found in the cloned region, which contained 725 base pairs upstream of the transcriptional initiation site of the Cx43 gene. The Cx43 promoter activity was found to be increased by incubation of the transfected cells with serum obtained from LPS-treated rats. Moreover, direct incubation of the transfected cells with LPS or interleukin 1 beta, but not with other cytokines, was observed to increase the Cx43 promoter activity. These results suggest the expression of Cx43 after administration of LPS is part of the inflammatory response. Moreover, the expression of this gene seems to be mediated by proinflammatory mediators