13 research outputs found
Radiological impact of phosphogypsum surface application in a no-till system in Southern Brazil
How Critical is Equity Capital? – Seasoned Equity Offerings in the Renewable Energy Sector
Über den Luxus von Kapitalerhöhungen: Ankündigungseffekte und asymmetrische Informationen in der Luxusgüterindustrie
Finanzierungsrestriktionen bei kleinen und mittelständischen Unternehmen der Umwelttechnologiebranche – Stand der Forschung und offene Fragen
J. Mol. Biol.
The regulation of ribosomal RNA biosynthesis in Escherichia coli by anti-termination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusB protein for boxA RNA exceeds that for the homologous DNA segment by more than three orders of magnitude as shown by surface plasmon resonance measurements. DNA RNA discrimination by NusB protein was shown to involve methyl groups (i.e. discrimination of uracil versus thymine) and 2' hydroxyl groups (i.e. discrimination of ribose versus deoxyribose side-chains) in the RNA motif. Ligand perturbation experiments monitored by (HN)-H-1-N-15 correlation NMR experiments identified amide NH groups whose chemical shifts are affected selectively by ribose/deoxyribose exchange in the 5' and the central part of the dodecameric boxA motif respectively. The impact of structural modification of the boxA motif on the affinity for NusB protein as observed by (HN)-H-1- N-15 heterocorrelation was analysed by a generic algorithm. (C) 2003 Elsevier Science Ltd. All rights reserved
RNA DNA discrimination by the antitermination protein NusB
The regulation of ribosomal RNA biosynthesis in Escherichia coli by anti-termination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusB protein for boxA RNA exceeds that for the homologous DNA segment by more than three orders of magnitude as shown by surface plasmon resonance measurements. DNA RNA discrimination by NusB protein was shown to involve methyl groups (i.e. discrimination of uracil versus thymine) and 2' hydroxyl groups (i.e. discrimination of ribose versus deoxyribose side-chains) in the RNA motif. Ligand perturbation experiments monitored by (HN)-H-1-N-15 correlation NMR experiments identified amide NH groups whose chemical shifts are affected selectively by ribose/deoxyribose exchange in the 5' and the central part of the dodecameric boxA motif respectively. The impact of structural modification of the boxA motif on the affinity for NusB protein as observed by (HN)-H-1- N-15 heterocorrelation was analysed by a generic algorithm. (C) 2003 Elsevier Science Ltd. All rights reserved