10 research outputs found
Effect of calcium and calcium antagonists on [3h]-paf-acether, binding to washed human platelets
Supplementary Material for: Noninvasive Prenatal Detection of Trisomy 21 by Targeted Semiconductor Sequencing: A Technical Feasibility Study
<p><b><i>Objective:</i></b> To develop an alternate noninvasive prenatal
testing method for the assessment of trisomy 21 (T21) using a targeted
semiconductor sequencing approach. <b><i>Methods:</i></b> A customized
AmpliSeq panel was designed with 1,067 primer pairs targeting specific
regions on chromosomes 21, 18, 13, and others. A total of 235 samples,
including 30 affected with T21, were sequenced with an Ion Torrent
Proton sequencer, and a method was developed for assessing the
probability of fetal aneuploidy via derivation of a risk score. <b><i>Results:</i></b>
Application of the derived risk score yields a bimodal distribution,
with the affected samples clustering near 1.0 and the unaffected near 0.
For a risk score cutoff of 0.345, above which all would be considered
at “high risk,” all 30 T21-positive pregnancies were correctly predicted
to be affected, and 199 of the 205 non-T21 samples were correctly
predicted. The average hands-on time spent on library preparation and
sequencing was 19 h in total, and the average number of reads of
sequence obtained was 3.75 million per sample. <b><i>Conclusion:</i></b>
With the described targeted sequencing approach on the semiconductor
platform using a custom-designed library and a probabilistic statistical
approach, we have demonstrated the feasibility of an alternate method
of assessment for fetal T21.</p
Navigating Toward Andragogy: Coordination and Management of Student–Professor Conversations
Co-ordinating meaning within a gender identity development service: What can the theory of the co-ordinated management of meaning offer clinicians working with young people, and their families, exploring their gender identities
Working with young people who present with diverse gender identifications and their families requires consideration and acknowledgement of a range of contexts (including relationship, family, social and cultural) as well as the influences of prior experience and language. Family members often present with diverse understandings and hopes from their referral to the Gender Identity Development Service and look to us to meet these. This article considers the contribution of Cronen and Pearce who introduced the theory of the co-ordinated management of meaning (CMM). We focus on their ideas about communication, the complexities of communication, meaning, context and multiple perspectives. This article relates to our practice as well as considers useful ways to work with families and young people, within a highly contested field. Clinical examples are used to illustrate how we have made use of the theory and models of CMM to facilitate engagement, build therapeutic rapport, facilitate understanding and support those we see in a way which takes into account the many contexts of their lives