Initial study for detection of multiple lymph nodes in the axillary region using Microwave Imaging

Breast cancer staging largely depends on the evaluation of whether there are cancer cells in the axillary lymph nodes. This work proposes a non-invasive method of detecting metastases in the lymph nodes using an Ultra-Wideband Microwave Imaging radar system. Energy profiles of the axilla are created with this system in which high energy regions may indicate the presence of metastasised lymph nodes.info:eu-repo/semantics/publishedVersio

Target Selection in Multistatic Microwave Breast Imaging Setup Using Dielectric Lens

Microwave Imaging (MWI) has been studied to aid early breast cancer detection. Current prototypes in more advanced stages of development include both monostatic or multistatic setups. However, multistatic configurations usually include a high number of antennas which consequently require complex and computationally-intensive signal processing algorithms to ensure a good target detection. We previously presented a novel approach using a dielectric lens which reduces the signal processing burden of multistatic setups, while ensuring good spatial resolution. In this paper, we evaluate this novel setup using an anatomically realistic breast phantom and its capability of selecting targets inside the breast. We show a successful detection of the targets using an artefact removal algorithm based on singular value decomposition when the Bessel beam is centered at the target location.info:eu-repo/semantics/publishedVersio

Experimental Evaluation of an Axillary Microwave Imaging System to Aid Breast Cancer Staging

The number of metastasised Axillary Lymph Nodes (ALNs) is a key indicator for breast cancer staging. Its correct assessment affects subsequent therapeutic decisions. Common ALN screening modalities lack high enough sensitivity and specificity. Level I ALNs produce detectable backscattering of microwaves, opening the way for Microwave Imaging (MWI) as a complementary screening modality. Radar-based MWI is a low-cost, noninvasive technique, widely studied for breast cancer and brain stroke detection. However, new specific challenges arise for ALN detection, which deter a simple extension of existing MWI methods. The geometry of the axillary region is more complex, limiting the antenna travel range required for maximum resolution. Additionally, unlike breast MWI setups, it is impractical to use liquid immersion to enhance energy coupling to the body; therefore, higher skin reflection masks ALNs response. We present a complete study that proposes dedicated imaging algorithms to detect ALNs dealing with the above constraints, and evaluate their effectiveness experimentally. We describe the developed setup based on a 3D-printed anthropomorphic phantom, and the antenna-positioning configuration. To the authors’ knowledge, this is the first ALN-MWI study involving a fully functional anatomically compliant setup. A Vivaldi antenna, operating in a monostatic radar mode at 2-5 GHz, scans the axillary region. Pre-clinical assessment in different representative scenarios shows Signal-to-ClutterRatio higher than 2.8 dB and Location Error lower than 15mm, which is smaller than considered ALN dimensions. Our study shows promising level I ALN detection results despite the new challenges, confirming MWI potential to aid breast cancer staging.info:eu-repo/semantics/publishedVersio

Feasibility study of focal lens for multistatic microwave breast imaging

Microwave Imaging is an emerging technique to aid breast cancer diagnosis. Current multistatic setups involve complex and heavy signal processing techniques, such as to remove the energy coupling between adjacent sensors, which masks the response from inner tissues. We investigate a novel approach using a dielectric lens in order to reduce the coupling effects between antennas, thus reducing the signal processing burden, while preserving all the advantages of multistatic setups. In this paper, we show that we can successfully detect simulated breast targets on reconstructed images using a setup with a dielectric lens.info:eu-repo/semantics/publishedVersio

Evaluation of Refraction Effects in Dry Medical Microwave Imaging Setups

Dry microwave imaging (MWI) systems are more practical, hygienic, and fast to operate since they do not require immersion liquid. However, the dielectric contrast between air and the part of the body under examination is larger, causing larger refraction effects. Including refraction in the image reconstruction algorithm significantly increases the computational effort, especially when imaging nonuniform shapes. Hence, our systematic study aims to evaluate the impact of neglecting refraction effects on MWI by using quantitative metrics and define objective guidelines that are lacking in the literature. We perform comparative studies with a spherical numerical phantom (which is typically used to represent simplified breast or head phantoms) by varying the phantom relative permittivity values between 4 and 40, metallic targets diameter between 5 and 15 mm, and the number of probing antennas. Additionally, the refraction effects are evaluated with anthropomorphic body phantoms representing a breast and the axillary region. We numerically and experimentally show that refraction tends to have a greater impact on imaging results when phantom relative permittivity values exceed 8, while it has a minor effect in the remaining tested cases. This favors potential fast real-time image reconstruction. This letter provides useful criteria to decide whether refraction should be considered or not for imaging reconstruction when developing new dry medical MWI setups.info:eu-repo/semantics/publishedVersio

Study of the Refraction Effects in Microwave Breast Imaging Using a Dry Setup

Medical Microwave Imaging (MWI) has been studied as a technique to aid breast cancer diagnosis. Several different prototypes have been proposed but most of them require the use of a coupling medium between the antennas and the breast, in order to reduce skin backscattering and avoid refraction effects. The use of dry setups has been addressed and recent publications show promising results. In this paper, we assess the importance of considering refraction effects in the image reconstruction algorithms. To this end, we consider a simplified homogeneous spherical model of the breast and analytically compute the propagating rays through the air-body interface. The comparison of results considering only direct ray propagation or refracted rays shows negligible impact on the accuracy of the images for moderately high permittivity media. Thus, we may avoid the computational burden of calculating the refracted rays in complex shapes.info:eu-repo/semantics/publishedVersio

Optimisation of Artefact Removal Algorithm for Microwave Imaging of the Axillary Region Using Experimental Prototype Signals

Microwave Imaging (MWI) has the potential to aid breast cancer staging through the detection of Axillary Lymph Nodes (ALNs). This type of system can present some challenges, mainly due to the irregular axillary surface. The optimisation of the artefact removal algorithm to successfully remove the surface reflections is of great importance. In this paper, we propose using Singular Value Decomposition (SVD) as an artefact removal algorithm and study the effect of choosing different subsets of antenna positions for artefact removal on imaging results using experimental signals. We show that different subsets of antenna positions affect the results and in some cases prevent the targets detection. Our analysis allowed us to find an optimal combination of parameters which results in Signal-to-Clutter Ratio higher than 2.77 dB and Location Error lower than 14.9 mm for three different experimental tests. These results are relevant for the development of dedicated algorithms for ALN-MWI application.info:eu-repo/semantics/publishedVersio

Application of error-prone PCR to functionally probe the morbillivirus Haemagglutinin protein.

The enveloped morbilliviruses utilise conserved proteinaceous receptors to enter host cells: SLAMF1 or Nectin-4. Receptor binding is initiated by the viral attachment protein Haemagglutinin (H), with the viral Fusion protein (F) driving membrane fusion. Crystal structures of the prototypic morbillivirus measles virus H with either SLAMF1 or Nectin-4 are available and have served as the basis for improved understanding of this interaction. However, whether these interactions remain conserved throughout the morbillivirus genus requires further characterisation. Using a random mutagenesis approach, based on error-prone PCR, we targeted the putative receptor binding site for SLAMF1 interaction on peste des petits ruminants virus (PPRV) H, identifying mutations that inhibited virus-induced cell-cell fusion. These data, combined with structural modelling of the PPRV H and ovine SLAMF1 interaction, indicate this region is functionally conserved across all morbilliviruses. Error-prone PCR provides a powerful tool for functionally characterising functional domains within viral proteins