Effects of the feeding challenge and age (median and IQR) on acute insulin response to glucose (AIRg), insulin sensitivity (IS), disposition index (DI) and glucose effectiveness (Sg), basal plasma glucose (GB) and basal plasma insulin (IB) in yearlings whose dams were fed with barley and forage (B, n = 10) or forage only (F, n = 10), at 19 and 24 months of age.

<p>Effects of the feeding challenge and age (median and IQR) on acute insulin response to glucose (AIRg), insulin sensitivity (IS), disposition index (DI) and glucose effectiveness (Sg), basal plasma glucose (GB) and basal plasma insulin (IB) in yearlings whose dams were fed with barley and forage (B, n = 10) or forage only (F, n = 10), at 19 and 24 months of age.</p

Mares' parameters from the 5^th gestational month to weaning in the five groups.

<p><b>A:</b> body weight. <b>B:</b> body scores. <b>C:</b> plasma NEFA. <b>D:</b> plasma leptin. (P-P: Pony in Pony (•), P-D: Pony in Draft (○), S-P: Saddlebred in Pony (▾), S-S: Saddlebred in Saddlebred (▪), S-D: Saddlebred in Draft (Δ)). Curves are presented as medians and interquartile ranges.</p

Foals' fasting glucose from birth to weaning in the five groups.

<p><b>A:</b> P-P (•) <i>vs</i> S-S (▪). <b>B:</b> P-P (•) <i>vs</i> P-D (○). <b>C:</b> S-P (▾) <i>vs</i> S-S (▪) <i>vs</i> S-D (Δ) (P-P: Pony in Pony, P-D: Pony in Draft, S-P: Saddlebred in Pony, S-S: Saddlebred in Saddlebred, S-D: Saddlebred in Draft). Curves are presented as medians and interquartile ranges. The median values under the asterisks differ significantly from each other (F1-LD-F1 model followed by Mann-Whitney or Kruskal-Wallis test, p<0.05). In graph C, median values under the simple and double asterisks significantly differ between S-P and S-S and between S-P and S-D, respectively.</p

Changes in the plasma concentrations of glucose and insulin in response to glucose bolus in the five groups.

<p><b>A:</b> Glycemia (A1) and insulinemia (A2) in P-P (•) <i>vs</i> P-D (○). <b>B:</b> Glycemia (B1) and insulinemia (B2) in S-P (▾) <i>vs</i> S-S (▪) <i>vs</i> S-D (Δ). <b>C:</b> Area under the curve for glucose (C1) and insulin (C2) in P-P (full yellow), P-D (chequered blue), S-P (striped pink), S-S (full green) and S-D (chequered red) (P-P: Pony in Pony, P-D: Pony in Draft, S-P: Saddlebred in Pony, S-S: Saddlebred in Saddlebred, S-D: Saddlebred in Draft). Curves are presented as medians and interquartile ranges. The median values under the asterisks differ significantly from each other (F1-LD-F1 model followed by Mann-Whitney or Kruskal-Wallis test, p<0.05). In graph B2, the asterisk indicates a significant difference between S-P and S-S.</p

Establishment of control and experimental pregnancies by artificial insemination (AI) and embryo transfer (ET), respectively.

<p>Establishment of control and experimental pregnancies by artificial insemination (AI) and embryo transfer (ET), respectively.</p

Foals' plasma T₃ levels from birth to weaning in the five groups.

<p><b>A:</b> P-P (full yellow) <i>vs</i> S-S (full green). <b>B:</b> P-P (full yellow) <i>vs</i> P-D (chequered blue). <b>C:</b> S-P (striped pink) <i>vs</i> S-S (full green) <i>vs</i> S-D (chequered red) (P-P: Pony in Pony, P-D: Pony in Draft, S-P: Saddlebred in Pony, S-S: Saddlebred in Saddlebred, S-D: Saddlebred in Draft). Curves are presented as medians and interquartile ranges and the scale on the y-axis is semi-logarithmic. The median values under the asterisks differ significantly from each other (F1-LD-F1 model followed by Mann-Whitney or Kruskal-Wallis test, p<0.05.</p

quality of feedstuff from INRA [29] given to yearlings during feeding challenge (19 to 24 months).

<p>quality of feedstuff from INRA [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169295#pone.0169295.ref029" target="_blank">29</a>] given to yearlings during feeding challenge (19 to 24 months).</p

Number of recipient and control mares and foals with sex ratio within the five groups.

<p>All mares were pregnant and delivered one foal, so mare numbers are the same as foal numbers.</p><p>(P-P: Pony in Pony, P-D: Pony in Draft, S-P: Saddlebred in Pony, S-S: Saddlebred in Saddlebred, S-D: Saddlebred in Draft).</p

Foals' plasma IGF-1 levels from birth to weaning in the five groups.

<p><b>A:</b> P-P (full yellow) <i>vs</i> S-S (full green). <b>B:</b> P-P (full yellow) <i>vs</i> P-D (chequered blue). <b>C:</b> S-P (striped pink) <i>vs</i> S-S (full green) <i>vs</i> S-D (chequered red) (P-P: Pony in Pony, P-D: Pony in Draft, S-P: Saddlebred in Pony, S-S: Saddlebred in Saddlebred, S-D: Saddlebred in Draft). Curves are presented as medians and interquartile ranges. The median values under the asterisks differ significantly from each other (F1-LD-F1 model followed by Mann-Whitney or Kruskal-Wallis test, p<0.05).</p

Summary of the number of foals included in each analysis.

<p>Summary of the number of foals included in each analysis.</p