Changes in cellular lipid composition over time in strains grown on glucose media.

<p><i>YALI</i>: <i>Yarrowia lipolytica</i> W29, <i>YAAL</i>: <i>C. alimentaria</i> CBS10151, <i>YAHI</i>: <i>C. hispaniensis</i> CBS9996. Quantities are in mg/g CDW.</p

Lipid content (% CDW) of <i>Yarrowia</i> strains over time.

<p>For each species, the histogram represents three different physiological states: 1/5 of maximum growth, 1/2 maximum growth and maximum growth. Error bars indicate the deviation from the mean deduced from two replicates. Analyses were performed on both glucose (A) and oleic acid (B). Strains are represented by an abbreviation of their species name, as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063356#pone-0063356-t001" target="_blank">Table 1</a>.</p

Growth capacities of <i>Yarrowia</i> strains on 13 hydrophobic substrates as a function of their phylogenetic position.

<p>Squares represent growth capacity on minimal and rich (YP) media supplemented with triglycerides in red, methylates in yellow, free fatty acids in green and alkanes in blue. The squares marked YP reflect the capacity to grow on the YP base used as a carbon source, in the presence of the corresponding HS. Half-colored squares indicate slow or weak growth. The absence of a square indicates an absence of growth on both YP and minimal media. The phylogenetic tree was constructed by the concatenation of seven markers (2204 amino acids) and its robustness was estimated with a bootstrap of 100 replicates.</p

Drop tests on methyl decanoate in MMB and YP media, after 9 days of culture.

<p>Three spots are shown, corresponding to approximately 25 to 625 cells, from right to left. Strains are represented by an abbreviation of their species name, as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063356#pone-0063356-t001" target="_blank">Table 1</a>.</p

Growth parameters on oleic acid and glucose media.

<p>Growth parameters on oleic acid and glucose media.</p

Drop tests on YP- and MMB-tributyrin media after 5 days of culture.

<p>Four spots are shown, with approximately 5 to 625 cells, from right to left. A halo is present around strains <i>YALI</i>, <i>YADE</i>, <i>YAOS</i>, <i>YAPH</i> and <i>YAHO</i>. Strains are represented by an abbreviation of their species name, as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063356#pone-0063356-t001" target="_blank">Table 1</a>.</p

Characteristics of the strains used in this study.

<p>Abbreviations: CBS, Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands;  = W29, other common name used;  = Y−5580, name from the NRRL collection.</p

Box-plot comparisons of the dN/dS ratio [ω] estimated in the various mt genes and based on pairwise alignments.

<p>Box-plot comparisons of the dN/dS ratio [ω] estimated in the various mt genes and based on pairwise alignments.</p

General features of the mitochondrial genomes of the <i>Lachancea</i> genus.

<p>General features of the mitochondrial genomes of the <i>Lachancea</i> genus.</p

Circular maps of the mitochondrial genomes.

<p>Protein-coding genes, tRNA and rRNA genes are presented in dark blue, red and green, respectively. Coding introns are represented in light blue, and non-coding introns in white. The <i>RPM1</i> gene is presented in yellow.</p