Chromosome spreads of male meiocytes in wild type and <i>Atpss1-1</i>.

<p>(A,G) Leptotene. (B,H) Pachytene. Arrowheads show unsynapsed regions. (C,I) Diakinesis. (D,J) Metaphase I. (E,K) Metaphase II. (F,L) Telophase II. Chromosome were spread according to Ross <i>et al.</i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Ross1" target="_blank">[40]</a> and stained with DAPI. Scale bar = 10 µm.</p

Co-immunolocalization of REC8 and ZYP1 at pachytene.

<p>Floral buds of the correct size or bigger for the late pachytene/diplotene stage in wild type were used to make spreads according to Armstrong <i>et al.</i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Armstrong1" target="_blank">[42]</a>. Scale bar = 10 µm. (A) Wild type. (B) <i>Atpss1-1</i>. (C) Histogram of cells according to their proportion of synapsed axes. The proportion of synapsed axes in each cell was estimated by measuring the frequency of [red and green] pixels among the total number of [red] pixels. For example on <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen-1004674-g003" target="_blank">figure 3B</a>, 51% of the ASY1 red signal (axes) colocalize with the ZYP1 green (synapsis) signal.</p

The <i>AtPSS1</i> gene and mutations.

<p>The arrow indicates the orientation of the open reading frame. Exons are shown as boxes (grey: UTR, black: CDS). In <i>Atpss1-1</i>, <i>Atpss1-2</i> and <i>Atpss1-3</i> corresponding to WiscDsLox_343E05, SALK_120399 and SALK_024926 lines, the T-DNA was inserted as indicated by triangles.</p

Average number of bivalents (blue) and pairs of univalents (red) per male meiocyte.

<p>Number of metaphase I cells analyzed is indicated in brackets.</p

MLH1 immunolocalization.

<p>Immunolocalization of MLH1 at diakinesis is shown (A) in wild type and (B) in <i>Atpss1-1</i>. (C, D). Scatter plot of MLH1 foci number per cell at diplotene and diakinesis. (E) Distribution of chromosomes according to their MLH1 foci number at diakinesis. Cells were prepared according to Chelysheva <i>et al.</i><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Chelysheva2" target="_blank">[47]</a>. Scale bar = 10 µm.</p

Genetic recombination in wild type and <i>Atpss1-1</i>.

<p>Genetic distances in six intervals using tetrad analysis with fluorescent-tagged lines (FTL), were calculated with the Perkins equation <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Perkins1" target="_blank">[67]</a> and are given in centiMorgans. I1b and I1c are adjacent intervals on chromosome 1 and so on for the other pairs of intervals as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Berchowitz2" target="_blank">[46]</a> (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674.s006" target="_blank">Table S1</a>).</p

Interaction between AtPSS1, AtWIPs and AtSUNs.

<p>Yeast two-hybrid - For each combination yeast cells were spotted on selective medium to test interactions (all combinations were able to grow on non-selective medium; not shown) ND: Not determined either because irrelevant or due to self-activation of one of the partners.</p

DMC1 immunolocalization.

<p>Immunolocalization of DMC1 at early zygotene is shown (A) in wild type and (B) in <i>Atpss1-1</i>. Cells were prepared according to Armstrong <i>et al</i>. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004674#pgen.1004674-Armstrong1" target="_blank">[42]</a> Scale bar = 10 µm. (C) Scatter plot of DMC1 foci number per cell.</p