OMD1 and OMD2 levels found in healthy and OA human sera.

<p>(A) OMD1, (B) OMD2. Data are presented as scatter dot plots with median from 22 patients in each group. Comparisons of values were performed by Mann Whitney test. (C) Spearman correlation between OMD1 and OMD2 in serum, n = 44.</p

Western-blotting detection of osteomodulin using goat polyclonal antisera raised against entire osteomodulin.

<p>(A)(B) rhOMD: human recombinant osteomodulin (positive control), DMEM: concentration unconditioned culture supernatant (negative control), NSC: non sclerotic osteoblasts supernatant (N36, N38, N40), SC: sclerotic osteoblasts supernatant (S36, S38, S40), C1-6: six healthy patients albumin/IgG depleted serum, OA1-6: six OA patients before total knee replacement surgery albumin/IgG depleted serum. (C) Quantification of osteomodulin western blot performed on osteoblasts culture supernatants and on albumin/IgG depleted serum of 6 healthy patients (controls) and 6 patients before total knee replacement (OA). Quantification was made using ImageJ on total band and normalized with total protein charged visualized with reverse total protein stain (Pierce).</p

Estimation of abundance of NSC osteoblasts secreted proteins using high top 3.

<p>Estimation of abundance of NSC osteoblasts secreted proteins using high top 3.</p

MS/MS proteomic analysis of osteoblasts secretome.

<p>MS/MS proteomic analysis of osteoblasts secretome.</p

Gene expression by NSC or SC osteoblasts.

<p>(A) less secreted proteins (B) more secreted proteins. The mRNA copy numbers were normalized against the corresponding copy number of HPRT mRNA and results are showed as the SC relative to NSC gene expression (mean ± SEM of five independent experiments performed with bone biopsy coming from different donors). In each experiment, each experimental condition was performed in duplicate. P: comparisons of mean values between NSC and SC were performed by unpaired t-test. Dotted blue line in each gene represents the result obtained with MS/MS in secretome with the corresponding protein.</p

OMD1 and OMD2 levels found in osteoblasts cell culture supernatant.

<p>Data are means +/- SEM from 10 patients. Comparisons of mean values were performed by unpaired t-test.</p

CXCL6 production by human chondrocytes in ctrl, COT, IL-1β and COT IL-1β conditions.

<p>Results were expressed as mean ± 95% confidence intervals of 12 independent experiments performed with chondrocytes isolated from 12 different patients. ***p-value<0.001. CXCL6 = chemokine (C-X-C motif) ligand 6.</p

MMP-13 and BMP-2 productions by human chondrocytes in ctrl, COT, IL-1β and COT IL-1β conditions.

<p>Results were expressed as mean ± 95% confidence intervals of 12 independent experiments performed with chondrocytes isolated from 12 different patients. **p-value<0.01, ***p-value<0.001. (a) MMP-13 = matrix metalloproteinase-13, (b) BMP-2 = bone morphogenetic protein-2, ND = non detectable.</p

STC1 and serpin E1 productions by human chondrocytes in ctrl, COT, IL-1β and COT IL-1β conditions.

<p>Results were expressed as mean ± 95% confidence intervals of 12 independent experiments performed with chondrocytes isolated from 12 different patients. *p-value<0.05, **p-value<0.01, ***p-value<0.001. (a) STC1 = stanniocalcin 1, (b) serpin E1</p