Density biases and temperature relations for DESIRED HII regions

We present a first study based on the analysis of the DEep Spectra of Ionized REgions Database (DESIRED). This is a compilation of 190 high signal-to-noise ratio optical spectra of HII regions and other photoionized nebulae, mostly observed with 8-10m telescopes and containing $\sim$29380 emission lines. We find that the electron density --$n_{\rm e}$-- of the objects is underestimated when [SII] $\lambda6731/\lambda6716$ and/or [OII] $\lambda3726/\lambda3729$ are the only density indicators available. This is produced by the non-linear density dependence of the indicators in the presence of density inhomogeneities. The average underestimate is $\sim 300$ cm$^{-3}$ in extragalactic HII regions, introducing systematic overestimates of $T_{\rm e}$([OII]) and $T_{\rm e}$([SII]) compared to $T_{\rm e}$([NII]). The high-sensitivity of [OII] $\lambda\lambda7319+20+30+31/\lambda\lambda3726+29$ and [SII] $\lambda\lambda4069+76/\lambda\lambda6716+31$ to density makes them more suitable for the diagnosis of the presence of high-density clumps. If $T_{\rm e}$([NII]) is adopted, the density underestimate has a small impact in the ionic abundances derived from optical spectra, being limited to up to $\sim$0.1 dex when auroral [SII] and/or [OII] lines are used. However, these density effects are critical for the analysis of infrared fine structure lines, such as those observed by the JWST in local star forming regions, implying strong underestimates of the ionic abundances. We present temperature relations between $T_{\rm e}$([OIII]), $T_{\rm e}$([ArIII]), $T_{\rm e}$([SIII]) and $T_{\rm e}$([NII]) for the extragalactic HII regions. We confirm a non-linear dependence between $T_{\rm e}$([OIII])-$T_{\rm e}$([NII]) due to a more rapid increase of $T_{\rm e}$([OIII]) at lower metallicities.Comment: Accepted for publication in MNRA

Generalized Toda Theory from Six Dimensions and the Conifold

Recently, a physical derivation of the Alday-Gaiotto-Tachikawa correspondence has been put forward. A crucial role is played by the complex Chern-Simons theory arising in the 3d-3d correspondence, whose boundary modes lead to Toda theory on a Riemann surface. We explore several features of this derivation and subsequently argue that it can be extended to a generalization of the AGT correspondence. The latter involves codimension two defects in six dimensions that wrap the Riemann surface. We use a purely geometrical description of these defects and find that the generalized AGT setup can be modeled in a pole region using generalized conifolds. Furthermore, we argue that the ordinary conifold clarifies several features of the derivation of the original AGT correspondence.Comment: 27+2 pages, 3 figure

Single-molecule experiments in biological physics: methods and applications

I review single-molecule experiments (SME) in biological physics. Recent technological developments have provided the tools to design and build scientific instruments of high enough sensitivity and precision to manipulate and visualize individual molecules and measure microscopic forces. Using SME it is possible to: manipulate molecules one at a time and measure distributions describing molecular properties; characterize the kinetics of biomolecular reactions and; detect molecular intermediates. SME provide the additional information about thermodynamics and kinetics of biomolecular processes. This complements information obtained in traditional bulk assays. In SME it is also possible to measure small energies and detect large Brownian deviations in biomolecular reactions, thereby offering new methods and systems to scrutinize the basic foundations of statistical mechanics. This review is written at a very introductory level emphasizing the importance of SME to scientists interested in knowing the common playground of ideas and the interdisciplinary topics accessible by these techniques. The review discusses SME from an experimental perspective, first exposing the most common experimental methodologies and later presenting various molecular systems where such techniques have been applied. I briefly discuss experimental techniques such as atomic-force microscopy (AFM), laser optical tweezers (LOT), magnetic tweezers (MT), biomembrane force probe (BFP) and single-molecule fluorescence (SMF). I then present several applications of SME to the study of nucleic acids (DNA, RNA and DNA condensation), proteins (protein-protein interactions, protein folding and molecular motors). Finally, I discuss applications of SME to the study of the nonequilibrium thermodynamics of small systems and the experimental verification of fluctuation theorems. I conclude with a discussion of open questions and future perspectives.Comment: Latex, 60 pages, 12 figures, Topical Review for J. Phys. C (Cond. Matt

N = 1 dualities in 2+1 dimensions

We consider minimally supersymmetric QCD in 2+1 dimensions, with Chern-Simons and superpotential interactions. We propose an infrared $SU(N) \leftrightarrow U(k)$ duality involving gauge-singlet fields on one of the two sides. It shares qualitative features both with 3d bosonization and with 4d Seiberg duality. We provide a few consistency checks of the proposal, mapping the structure of vacua and performing perturbative computations in the $\varepsilon$-expansion

Maternal Infection with Trypanosoma cruzi and Congenital Chagas Disease Induce a Trend to a Type 1 Polarization of Infant Immune Responses to Vaccines

Vaccines are of crucial importance to prevent morbidity and mortality due to infectious diseases in childhood. A modulation of the fetal/neonatal immune system (considered immature) toward Th1 or Th2 dominance could modify responses to vaccines administered in early life. T. cruzi is the agent of Chagas' disease, in Latin America currently infecting about 2 million women at fertile ages who are susceptible to transmitting the parasite to their fetus. In previous studies we showed that T. cruzi-infected mothers can induce a pro-inflammatory environment in their uninfected neonates (M+B−), whereas congenitally infected newborns (M+B+) are able to develop a pro-Th1 parasite-specific T cell response. In the present study, we analysed the cellular and/or antibody responses to Bacillus Calmette Guerin (BCG), hepatitis B birus (HBV), diphtheria and tetanus vaccines in 6- to 7-month-old infants living in Bolivia. M+B− infants produced more IFN-γ in response to BCG, whereas M+B+ infants developed a stronger IFN-γ response to hepatitis B, diphtheria and tetanus vaccines and enhanced antibody production to HBs antigen. These results show that both maternal infection with T. cruzi and congenital Chagas disease do not interfere with responses to BCG, hepatitis B, diphtheria and tetanus vaccines in the neonatal period and that T. cruzi infection in early life tends to favour type 1 immune responses to vaccinal antigens

Type 2 Diabetes Variants Disrupt Function of SLC16A11 through Two Distinct Mechanisms

Type 2 diabetes (T2D) affects Latinos at twice the rate seen in populations of European descent. We recently identified a risk haplotype spanning SLC16A11 that explains ∼20% of the increased T2D prevalence in Mexico. Here, through genetic fine-mapping, we define a set of tightly linked variants likely to contain the causal allele(s). We show that variants on the T2D-associated haplotype have two distinct effects: (1) decreasing SLC16A11 expression in liver and (2) disrupting a key interaction with basigin, thereby reducing cell-surface localization. Both independent mechanisms reduce SLC16A11 function and suggest SLC16A11 is the causal gene at this locus. To gain insight into how SLC16A11 disruption impacts T2D risk, we demonstrate that SLC16A11 is a proton-coupled monocarboxylate transporter and that genetic perturbation of SLC16A11 induces changes in fatty acid and lipid metabolism that are associated with increased T2D risk. Our findings suggest that increasing SLC16A11 function could be therapeutically beneficial for T2D. Video Abstract [Figure presented] Keywords: type 2 diabetes (T2D); genetics; disease mechanism; SLC16A11; MCT11; solute carrier (SLC); monocarboxylates; fatty acid metabolism; lipid metabolism; precision medicin