VALIDATED REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF ARTEMETHER AND LUMEFANTRINE IN FIXED COMBINED DOSAGE FORM

ABSTRACTObjective: The present aim is to develop simple, precise, and accurate reverse phase high performance liquid chromatographic method (RP-HPLC) forthe simultaneous assay of artemether and lumefantrine in fixed combined dosage form.Methods: The chromatographic study was carried out on Hypersil C18 column (250×4.6 mm, 5 μ) with mobile phase containing a mixture of KH2PO4buffer (pH-3.5) and acetonitrile in the ratio of 45:55% v/v at a flow rate of 1.0 ml/minute with ultraviolet detection at 218 nm in ambient columntemperature.Results: Using the optimized chromatographic conditions artemether and lumefantrine eluted with retention times of 2.207 and 3.733 minutes,respectively. The method was validated according to ICH guidelines with good reproducibility and linear responses, y=60.813.x+629.53 (r²=0.9982)for artemether and y=88.3108.x+2370.2 (r²=0.9912). The % relative standard deviations of intra-day precision was ranged 0.378% and 1.26% forartemether and 0.459% and 1.15% for lumefantrine, respectively. The percentage recoveries were ranged from 99.96% to 100.02% for artemetherand 99.96-99.97% for lumefantrine, respectively.Conclusions: The developed RP-HPLC method was validated as per ICH guidelines and was found to be best suitable for pharmacokinetic studies ofthese mentioned drugs.Keywords: Artemether, Lumefantrine, ICH guidelines

USE OF ION ASSOCIATION COMPLEX FORMATION FOR THE SPECTROPHOTOMETRIC DETERMINATION OF ITOPRIDE HCL IN BULK AND ITS PHARMACEUTICAL PREPARATIONS

Objective: The authors report two simple, accurate and economic spectrophotometric methods A and B for the determination of Itopride hydrochloride in bulk and dosage forms.Methods: The proposed methods are based on the formation of chloroform soluble ion-associates in the presence of acidic dyes namely BPB (Method A) and BCP (Method B) exhibiting lmax at 418 and 418 nm respectively.Results: Beer's law is found to be obeyed in the concentration range of 2.0-10.0 µg/ml and 2.0-10.0 µg/ml. The molar absorptivities are found to be 1.42x104 and 9.61x103L/mol. cm for methods A and B. These methods are successfully applied for the assay of Itopride hydrochloride in pharmaceutical formulations

A NOVEL MULTIPHASE BIDIRECTIONAL FLY-BACK CONVERTER TOPOLOGY IS APPLIED TO INDUCTION MOTOR DRIVE

Hybrid Electric Vehicle (HEV) is an emerging technology in the modern world because of the fact that it mitigates environmental pollutions and at the same time increases fuel efficiency of the vehicles. Bi-directional Fly – back Converter controls electric drive of HEV of high power and enhances its performance which is the reflection of the fact that it can generate Constant voltages. For hybrid electric vehicles, the batteries and the drive dc link may be at different voltages. The batteries are at low voltage to obtain higher volumetric efficiencies, and the dc link is at higher voltage to have higher efficiency on the motor side. Therefore, a power interface between the batteries and the drive’s dc link is essential. This power interface should handle power flow from battery to motor, motor to battery, external gen-set to battery, and grid to battery. This paper proposes a multi-power-port topology which is capable of handling multiple power sources and still maintains simplicity and features like obtaining high gain, wide load variations, lower output-current ripple, and capability of parallel-battery energy due to the modular structure. The scheme incorporates a transformer winding technique which drastically reduces the leakage inductance of the coupled inductor. The development and testing of a bidirectional fly-back dc–dc converter for hybrid electric vehicle is described in this paper. Simple hysteresis voltage control is used for dc-link voltage regulation. The simulation results are presented, and modeling the circuit by using MATLAB/SIMULINK Platform

4-[(4-Methyl­benz­yl)amino]-3-[(4-methyl­benz­yl)imino­meth­yl]-2H-chromen-2-one

The title compound, C26H24N2O2, was prepared from the reaction of 4-chloro-3-formyl­coumarin with p-methyl­benzyl­amine. Even though there are no strong and specific inter­actions in the crystal structure, the translationally related mol­ecules form chains along the b axis. The coumarin moieties are stacked through π–π inter­actions [centroid–centroid distance = 3.5275 (7) Å], forming layers perpendicular to the stacking direction

A Reverse Phase HPLC Method Development and Validation for the Determination of Paliperidone in Pure and Dosage Forms

Abstract: A simple reverse phase HPLC method was developed and validated for the assay of paliperidone in pure and tablets. The analysis was carried out on Shimadzu HPLC model equipped with inertsil ODS C-18 column, LC-10AT pump, variable wavelength programmable UV-Detector SPD-10AVP and rheodyne injector with 20µL fixed loop. Acetonitrile and methanol in the ratio 10:90 v/v at a flow rate of 1.0 mL/min was employed for elution and the components were detected at 235 nm at ambient temperature. The method was validated as per the ICH guidelines. The retention time for paliperidone was found to be 5.67 min. Linearity was observed in the concentration range of 20-120 ppm with correlation coefficient 0.9990. The percent of relative standard deviation of six replicate measurements was found to be 1.25 indicate that the proposed method was precise. Recovery studies were conducted at three different concentration levels within the linearity limits and the average percent of recovery was 99.56. The amount of paliperidone drug content present in tablet dosage form was determined and found to be 99.80%. Therefore, the developed method was simple, precise and accurate and can be successfully applied for the estimation of paliperidone in pharmaceutical dosage form

Greener One-pot Synthesis of Chromeno Oxazin and Oxazin Quinoline Derivatives and their Antibacterial Activity

An efficient green method for the synthesis of oxazino quinoline-2-amine derivatives, oxazino quinoline derivatives and chromeno oxazin-5-one derivatives have been synthesized through cyclization of aromatic aldehyde, ammonium acetate, substituted amides and 8-hydroxy-quinoline or 4-hydroxy coumarin by one-pot condensation method is described. The synthesized compounds are characterized by FT-IR, 1H NMR and MASS spectral techniques and are screened further for biological activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using cup plate method and disc diffusion method

Theories of Ultrasonic Velocities and their Application in Binary Liquid Mixtures of N-Ethylaniline with Some Toluenes (Toluene, o-Nitrotoluene and m-Nitrotoluene)

Abstract: Ultrasonic velocities and densities of the binary liquid mixtures of N-ethyl aniline with different toluenes like toluene, o-nitrotoluene and m-nitrotoluene have been measured at temperatures 303.15 and 308.15 K over the entire composition range. Various theories of ultrasonic velocity were applied to experimental values in evaluating the velocities using Nomoto's relation (U NR ), Impedence relation (U IR ), Ideal mixing relation (U IMR ), Jungie's relation (U JR )and Rao's specific velocity relation (U R ). The molecular interaction parameter (χ) has been evaluated from the values of experimental and theoretical velocities. The variation of this interaction parameter with the composition mixture has been discussed in terms of molecular interactions

Extractive Visible Spectrophotometric Determination of Lamotrigine in Pure and Pharmaceutical Formulations

Abstract: Two simple, sensitive, accurate and economical spectrophotometric methods (A & B) for the determination of lamotrigine (LMT) in pure and pharmaceutical formulations (tablets) are described in the present work. Method A is based on the formation of coloured ion-association complex between lamotrigine and tropaeolineo-oo (TPooo) with absorption maximum at 485 nm and obeys Beer's law in the concentration range of 5-25 µg/mL. Formation of ion-association complex between lamotrigine and alizarine Red S (ARS) to yield a coloured chromogen exhibiting absorption maximum at 440 nm (Method B) obeying Beer's law in the concentration range of 5-25 µg/mL forms the basis of Method B. The two methods have been validated statistically through recovery studies. The results are compared with those obtained using UV spectrophotometric method (Reference method) in chloroform at 260 nm

Hydrothermal Synthesis, Microstructure and Photoluminescence of Eu3+-Doped Mixed Rare Earth Nano-Orthophosphates

Eu3+-doped mixed rare earth orthophosphates (rare earth = La, Y, Gd) have been prepared by hydrothermal technology, whose crystal phase and microstructure both vary with the molar ratio of the mixed rare earth ions. For LaxY1–xPO4: Eu3+, the ion radius distinction between the La3+ and Y3+ is so large that only La0.9Y0.1PO4: Eu3+ shows the pure monoclinic phase. For LaxGd1–xPO4: Eu3+ system, with the increase in the La content, the crystal phase structure of the product changes from the hexagonal phase to the monoclinic phase and the microstructure of them changes from the nanorods to nanowires. Similarly, YxGd1–xPO4: Eu3+, Y0.1Gd0.9PO4: Eu3+ and Y0.5Gd0.5PO4: Eu3+ samples present the pure hexagonal phase and nanorods microstructure, while Y0.9Gd0.1PO4: Eu3+ exhibits the tetragonal phase and nanocubic micromorphology. The photoluminescence behaviors of Eu3+ in these hosts are strongly related to the nature of the host (composition, crystal phase and microstructure)