Search and dissemination in data processing

Manual retrieval methods were used to complete 54 searches of interest for the General Aviation Newsletter. Subjects of search ranged from television transmission to machine tooling, Apollo moon landings, electronic equipment, and aerodynamics studies

Technology utilization in a non-urban region: Further impact and technique of the Technology Use Studies Center

Updated information pertaining to clients who receive and use information disseminated by the Technology Use Studies Center (TUSC) is reported. Charts are provided which indicate TUSC's performance in information dissemination and technical assistance in terms of quantities of searches accomplished during several contract years. The faculty information service is described, along with details of cooperation with other agencies. Specific searches are listed according to subject, client, and client location, and a measure of client response to services provided is indicated by the included selection of transfer and impact reports. The impetus behind the formation of the general aviation news letter is also described

Technology utilization in a non-urban region: Further impact and technique of the Technology Use Studies Center, 2

The clientele served by the Technology Use Studies Center (TUSC) is updated. Manufacturing leads the list of client firms. The standard industrial classification (SIC) range of these firms is broad. Substantial numbers of college and university faculties are using TUSC services. Field operations inherent in the functions of dissemination and assistance are reviewed. Increasing emphasis among clientele is on environmental concerns and management. A record is provided of the institutions contacted and the extent of TUSC involvement with them, as well as TUSC's cooperation with agencies and organizations. The impact of TUSC and the NASA-sponsored Technology Utilization Program on other public agencies is discussed

Technology utilization

Documentation is presented for selected transfer and impact reports provided to the business community, government agencies, and such other requestors as schools, universities, and health services. Statistical data are also included on the characteristics of the TUSC technical searches

Technology utilization data searches

Technology Use Studies Center activities, functions, and services are reported for this period. Transfers and searches are described. Characteristics of TUSC searches are tabulated

b-quark decay in the collinear approximation

The semileptonic decay of a b-quark, b--> c l nu, is considered in the relativistic limit where the decay products are approximately collinear. Analytic results for the double differential lepton energy distributions are given for finite charm-quark mass. Their use for the fast simulation of isolated lepton backgrounds from heavy quark decays is discussed.Comment: 7 pages, 1 figure, submitted to Phys.Rev.

ScreenMill: A freely available software suite for growth measurement, analysis and visualization of high-throughput screen data

<p>Abstract</p> <p>Background</p> <p>Many high-throughput genomic experiments, such as Synthetic Genetic Array and yeast two-hybrid, use colony growth on solid media as a screen metric. These experiments routinely generate over 100,000 data points, making data analysis a time consuming and painstaking process. Here we describe <it>ScreenMill</it>, a new software suite that automates image analysis and simplifies data review and analysis for high-throughput biological experiments.</p> <p>Results</p> <p>The <it>ScreenMill</it>, software suite includes three software tools or "engines": an open source <it>Colony Measurement Engine </it>(<it>CM Engine</it>) to quantitate colony growth data from plate images, a web-based <it>Data Review Engine </it>(<it>DR Engine</it>) to validate and analyze quantitative screen data, and a web-based <it>Statistics Visualization Engine </it>(<it>SV Engine</it>) to visualize screen data with statistical information overlaid. The methods and software described here can be applied to any screen in which growth is measured by colony size. In addition, the <it>DR Engine </it>and <it>SV Engine </it>can be used to visualize and analyze other types of quantitative high-throughput data.</p> <p>Conclusions</p> <p><it>ScreenMill </it>automates quantification, analysis and visualization of high-throughput screen data. The algorithms implemented in S<it>creenMill </it>are transparent allowing users to be confident about the results <it>ScreenMill </it>produces. Taken together, the tools of <it>ScreenMill </it>offer biologists a simple and flexible way of analyzing their data, without requiring programming skills.</p

Using in vivo-biotinylated ubiquitin to describe a mitotic exit ubiquitome from human cells

Mitotic division requires highly regulated morphological and biochemical changes to the cell. Upon commitment to exit mitosis, cells begin to remove mitotic regulators in a temporally and spatially controlled manner to bring about the changes that re-establish interphase. Ubiquitin-dependent pathways target these regulators to generate polyubiquitin-tagged substrates for degradation by the 26S proteasome. However, the lack of cell-based assays to investigate in vivo ubiquitination limits our knowledge of the identity of substrates of ubiquitin-mediated regulation in mitosis. Here we report an in vivo ubiquitin tagging system in human cells that allows efficient purification of ubiquitin conjugates from synchronised cell populations. Coupling purification with mass spectrometry, we identify a series of mitotic regulators that are targeted for polyubiquitination in mitotic exit. We show that some are new substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading. We conclude that in vivo biotin-tagging of ubiquitin can provide valuable information about the role of ubiquitin-mediated regulation in processes required for rebuilding interphase cells

Evaluation of Antiplatelet-Related Bleeding Events in a Community Hospital

Background:Bleeding rate among patients who take high-dose aspirin is approximately 1.92% with an expected 2-3 fold increase after a second antiplatelet is added. Similarly, the addition of an anticoagulant to single or dual antiplatelet therapy results in increased bleeding rates of approximately 13.9% and 15.7% respectively. The concomitant use of antiplatelets and anticoagulants has become a common practice due to the high incidence of clinical conditions for which these agents are used. Hence, reversal of antithrombotic agents is often needed during major bleeding events or invasive procedures. However, the use of antiplatelet reversal is still under-utilized, and its efficacy on patient outcomes remains to be tested. The purpose of this project is to assess the incidence of bleeding, reversal strategies, mortality rates, and thrombotic events in patients with a major bleeding event receiving antiplatelets with or without concomitant anticoagulants. Methods: A retrospective chart review was conducted over a one-year period. Patients 18 years and older taking an antiplatelet excluding low dose aspirin alone at the time of the major bleeding event were included in the study. A major bleeding event was defined as bleeding into a critical area/organ, fall in hemoglobin of ≥ 20 g/L, or transfusion of \u3e 2 units of blood. Subjects were divided into two groups: intracranial and non-intracranial hemorrhage. Patient outcomes will be analyzed based on bleeding severity, causative agents, and reversal strategies utilized. Results: We identified 21 patients with ICH and 38 with Non-ICH. Bleeding rates among patients who had a major bleeding event were as follows: 12.4% of patients on antiplatelet therapy, 2.4% on APT monotherapy, 5.4% on DAPT, and 4% on APT plus an anticoagulant. A reversal strategy was utilized 68% of the time for the reversal of antiplatelets in the setting of an ICH compared to 18% in the Non-ICH group. Our findings suggest that mortality, readmission, and thrombotic events are not affected by bleeding severity or reversal strategy utilized. Discussion: Antiplatelet therapy with or without anticoagulation could increase the risk for a major bleeding event. At Baptist Hospital of Miami, antiplatelet reversal is conducted in accordance with current societal guideline recommendations. However, there is opportunity for improvement in the reversal of antiplatelet agents when utilized concomitantly with an anticoagulant