IDENTIFICATION OF ANTIRHEUMATOID ARTHRITIS LIGANDS USING IN SILICO ANALYSIS

Objective: The purpose of the research was to implement molecular docking simulation to investigate the anti-inflammatory capability of against thioesterase-2, an essential component of the inflammatory reaction in humans. Methods: After obtaining three-dimensional structures of arthritis ligand and thioesterase-2 protein from IUPHAR/BPS Guide to PHARMACOLOGY, PUBCHEM, and RCSB PDB databases server, ligands with suitable absorption, distribution, metabolism, and excretion (ADME) properties were docked against thioesterase-2 protein using PyRx 0.8 and AutoDock tools 1.5.7. Results: Indomethacin showed better binding affinity to thioesterase-2. Conclusion: In light of its promising binding affinities with thioesterase-2, indomethacin could be taken into consideration as a prospective pharmacological agent for thioesterase-2 inhibition, according to molecular docking simulation and SWISS ADME evaluation

Antimicrobial activity of some medicinal plants used for the treatment of tuberculosis in the Eastern Cape Province, South Africa

Artemisia afra Jacq. Ex Willd., Carpobrotus edulis L. and Tulbaghia violacea Harv. were screened for activity against Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Mycobacterium aurum A+ strain using a two-fold microdilution bioassay. M. aurum is tuberculosis (TB)related strain that was used in this study. These plants were selected based on their use by South African traditional healers for the treatment of TB and symptoms of the disease. All three plants were extracted with water, ethanol and dichloromethane. The extracts of A. afra were found to be activeagainst all the tested microorganisms. Only in the instance of A. afra and C. edulis did water extract show activity against M. aurum A+ strain. The ethanol extract of C. edulis showed very good activity against the Gram-positive bacteria only. Dichloromethane extracts of T. violacea were found to behighly active against all the microorganisms, except for K. pneumoniae

Production and evaluation of a TiO2 based 68Ge/68Ga generator

>Magister Scientiae - MSc68Ge/68Ga generators rely on metal oxide, inorganic and organic sorbents in order to prepare radionuclides useful for clinical applications. The requirements for 68Ge/68Ga generators are that the 68Ga obtained from the 68Ge loaded column should be optimally suited for the routine synthesis of 68Ga-labelled radiopharmaceuticals, that the separation of the 68Ga daughter from the 68Ge parent should happen easily, with a high yield of separation, a low specific volume of 68Ga and should not contain trace elements owing to the solubility of the metal oxide sorbent. Beginning with a metal oxide preparation and continuing through recent developments, several approaches for processing generator derived 68Ga have altered the production of 68Ge/68Ga generators. Still, the effects of sorbent modification on the properties of 68Ge/68Ga radionuclide generator systems are not necessarily optimally designed for direct application in a medical context. The objective of this research was to analyze and document characteristics of Titanium Oxide (TiO2) sorbents relevant to processing of a 68Ge/68Ga generator that is able to produce 68Ga eluates that are adequate for clinical requirements. Interest was shown in TiO2 based 68Ge/68Ga generators by a number of overseas companies for tumour imaging using 68Ga-labelled 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-conjugated peptides. While a method involving production of the 68Ga radionuclide using TiO2 metal oxide had been published, problems with the production persisted. A method, using TiO2 metal oxide for ion exchange chromatography, was devised in this study to produce the 68Ga radionuclide, with the aim of being adopted for production purposes. The study focuses on the development of a dedicated procedure for the achievement of sufficient 68Ga yield along with low 68Ge breakthrough and low metallic impurities. Literature from 1970 to 2011 was reviewed to assess the radiochemical aspects of the 68Ga production and processing thereof. Various commercially available TiO2 metal oxides were characterized by subjecting the materials to x-ray diffraction (XRD), x-ray fluorescence (XRF) and scanning electron microscopy (SEM) for quantitative and qualitative analysis

Confirmation of a slow symplasmic loading and unloading pathway in barley (Hordeum Vulgare L.) source and sink leaves

Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma

A mini-thesis submitted to the School of Government, Faculty of Economic and Management

Masters in Public Administration - MPAGreater capacity for cities to be disaster resilient is needed. Public-private partnership enhances municipal capacity. This study therefore, interrogates the relationship between the City of Cape Town and its disaster relief partners. It is mainly concerned with policy implementation, and as such, investigates disaster management policy implementation in relation to public-private partnerships. The objectivesof the study were to provide a conceptual framework that defines the terms eminent in the practice and study of disaster management, particularly those that characterize public-private partnerships in the City of Cape Town; to explore the legislative mandate that makes provisions for disaster management and the funding of public-private partnerships in municipalities; to document the current practice of public-private partnerships in the City of Cape Town; to highlight noted challenges in the partnership and where necessary, recommend alternative policy implementation options for enhanced partnership sustainable capacity for disaster relief. The researcher deemed it expedient to utilize the qualitative method for the purposes of the adeptness it affords in expediting malleable, arduous investigation in engaging with the phenomena that “unfold[s] in real-world situations.” As far as the findings of this study are concerned, the public-private partnership of the City of Cape Town with its disaster relief partners is a successful one. It is effective in bringing the much needed awareness and relief to devastated communities. There are four main challenges that impact on this disaster relief partnership have been realized by this study. These namely are the delays in the reimbursement process, the constant change of government personnel dealing with relief partners, absence of a disaster relief official on the sites of distress and unsubsidized expenses incurred by the relief partners. Having realized these challenges, the study makes policy implementation recommendations

Understanding the role of perforations on the local hydrodynamics of gas–liquid flows through structured packings

Structured packings are widely used to perform gas–liquid separation processes as they provide a large mass-transfer area and low-pressure drop. The overall performance of these packings is governed by the local liquid distribution/gas–liquid interfacial area. The local liquid distribution is substantially influenced by the arrangement and geometrical features of the structured packing. While the influence of arrangement and few geometrical features on the local liquid distribution has recently been investigated using the structure-resolved simulations, the perforations on the structured packings are usually ignored. In the present work, we have performed structure-resolved gas–liquid flow simulations, using the Volume-of-Fluid (VOF) method implemented in the open-source C++ library OpenFOAM, in a periodic domain of Mellapak.250 with and without resolving the perforations. We show that the presence of perforations results in flow separation as well as droplet formation leading to an increase in the liquid holdup, interfacial and wetted areas, irrespective of the fluid properties and wetting conditions (specified using static contact angle ‘θw’) considered in this work. We also show that at an inclination angle ‘β’ of 45° from horizontal, the location of the perforations governs the local liquid distribution and the resulting flow metrics. However, at a β of 90°, the number of perforations governs the local liquid distribution, irrespective of their location. Further, we also show that the predictions of structure-resolved VOF simulations, with perforations resolved, are in a relatively better agreement with the correlations in the literature at small values of ‘θw’) in terms of liquid holdup and interfacial area.<br/

Identification of the Anti Listerial

Partially purified fractions of the n-hexane extract of Garcinia kola seeds were obtained through column chromatography and their constituents were identified through the use of gas chromatography coupled to mass spectrometry (GC-MS). Three fractions were obtained by elution with benzene as the mobile phase and silica gel 60 as the stationery phase and these were named Benz1, Benz2, and Benz3 in the order of their elution. The antiListerial activities of these fractions were assessed through MIC determination and only Benz2 and Benz3 were found to be active with MIC’s ranging from 0.625 to 2.5 mg/mL. The results of the GC-MS analysis showed Benz2 to have 9 compounds whilst Benz3 had 7 compounds, with the major compounds in both fractions being 9,19-Cyclolanost-24-en-3-ol, (3.β.) and 9,19-Cyclolanostan-3-ol,24-methylene-, (3.β.). The Benz2 fraction was found to have mainly indifferent interactions with ampicillin and penicillin G whilst mainly additive interactions were observed with ciprofloxacin. The Benz3 fraction’s interactions were found to be 50% synergistic with penicillin G and 25% synergistic with ciprofloxacin and ampicillin. A commercially available 9,19-Cyclolanost-24-en-3-ol, (3.β.) was found not to exhibit any antiListerial activities at maximum test concentrations of 5 mg/mL, suggesting that the compound could be acting in synergy with the other compounds in the eluted fractions of Garcinia kola seeds