Spray Formation and Cavitation of Fuel Injectors with Various Metal and Optical Nozzles

This thesis addresses the need for fundamental understanding of the mechanisms of fuel spray formation and mixture preparation in direct injection spark ignition (DISI) engines. Fuel injection systems for DISI engines undergo rapid developments in their design and performance, therefore, their spray breakup mechanisms in the physical conditions encountered in DISI engines over a range of operating conditions and injection strategies require continuous attention. In this context, there are sparse data in the literature on spray formation differences between conventionally drilled injectors by spark erosion and latest laser drilled injector nozzles. A comparison was first carried out between the holes of spark eroded and laser-drilled injectors of same nominal type by analysing their in-nozzle geometry and surface roughness under an electron microscope. Then the differences in their spray characteristics under quiescent conditions, as well as in a motoring optical engine, are discussed on the basis of high-speed imaging experiments and image processing methods. Specifically, the spray development mechanism was quantified by spray tip penetration and cone angle data under a range of representative low-load and high-low engine operating conditions (0.5 bar and 1.0 bar absolute, respectively), as well as at low and high injector body temperatures (20 °C and 90 °C) to represent cold and warm engine-head conditions. Droplet sizing was also performed with the spark and laser drilled injectors using Phase Doppler Anemometry in a quiescent chamber and the analysis was extended to include flash boiling conditions (120 °C) and other hydrocarbon and alcohols; iso-octane, ethanol and butanol. This thesis also presents the design and development of a real-size quartz optical nozzle, 200 µm in diameter, suitable for high-temperature applications and also compatible with new fuels such as alcohols. Mass flow of typical real multi-hole injectors was measured, and relevant fluid mechanics dimensionless parameters were derived. Laser and mechanical drilling of the quartz nozzle holes were compared to each other. Abrasive flow machining of the optical nozzles was also performed and analysed by microscopy in comparison to the real injector. Results with a highspeed camera showed successful imaging of microscopic in-nozzle flow and cavitation phenomena, coupled to downstream spray formation, under a variety of conditions including high fuel temperature flash-boiling effects, although undesirable needle movement was an issue and limitation

Analysis of in-cylinder engine flows and their constituents by proper orthogonal decomposition

Analysis of in-cylinder engine flows and their constituents by proper orthogonal decompositio

Cross-correlation of POD spatial modes for the separation of stochastic turbulence and coherent structures

This article describes a proper-orthogonal-decomposition (POD) based methodology proposed for the identification and separation of coherent and turbulent velocity fluctuations. Typically, POD filtering requires assumptions to be made on the cumulative energy content of coherent modes and can therefore exclude smaller, but important contributions from lower energy modes. This work introduces a suggested new metric to consider in the selection of POD modes to be included in a reconstruction of coherent and turbulent features. Cross-correlation of POD spatial modes derived from independent samples is used to identify modes descriptive of either coherent (high-correlation) or incoherent (low-correlation) features. The technique is demonstrated through application to a cylinder in cross-flow allowing appropriate analysis to be carried out on the coherent and turbulent velocity fields separately. This approach allows identification of coherent motions associated with cross-flow transport and vortex shedding, such as integral length scales. Turbulent flow characteristics may be analysed independently from the coherent motions, allowing for the extraction of properties such as turbulent length scale

Towards in-cylinder flow informed engine control strategies using linear stochastic estimation

Many modern I.C. engines rely on some form of active control of injection, timing and/or ignition to help combat tailpipe out emissions, increase the fuel economy and engine drivability. However, development of these strategies is often optimised to suit the average cycle at each condition; an assumption that can lead to sub-optimal performance, especially an increase in particulate (PN) emissions as I.C. engine operation, and in-particular it’s charge motion is subject to cycle-to-cycle variation (CCV). Literature shows that the locations of otherwise repeatable large-scale flow structures may vary by as much 25% of the bore dimension; this could have an impact on fuel break-up and distribution and therefore subsequent combustion performance and emissions. In the presented work, a method is presented that allows full-field flow velocity information to be estimated in real-time from only a limited number of point velocity measurements using linear stochastic estimation (LSE). Three sensor arrangements – single bisecting ‘line-of-sight’, a central cluster and a circumferential ring - which are deemed applicable to implementation in an I.C. engine are compared over all test flow conditions; with all providing useful estimations of the flow field. It is shown how with even a modest number of point measurements it is possible to achieve at least 85% correlation between estimates and original data allowing cycle characterisation to be achieved. Information gathered from this technique could provide inputs to engine control strategies to account for the CCV of the in-cylinder flow

Spurious PIV vector correction using Linear Stochastic Estimation

Techniques for the experimental determination of velocity fields such as particle image velocimetry (PIV) can often be hampered by spurious vectors or sparse regions of measurement which may occur due to a number of reasons. Commonly used methods for detecting and replacing erroneous values are often based on statistical measures of the surrounding vectors and may be influenced by further poor data quality in the region. A new method is presented in this paper using Linear Stochastic Estimation for vector replacement (LSEVR) which allows for increased flexibility in situations with regions of spurious vectors. LSEVR is applied to PIV dataset to demonstrate and assess its performance relative to commonly used bilinear and bicubic interpolation methods. For replacement of a single vector, all methods performed well, with LSEVR having an average error of 11% in comparison to 14% and 18% for bilinear and bicubic interpolation respectively. A more significant difference was found in replacement of clusters of vectors which showed average vector angle errors of 10°, 9° and 6° for bilinear, bicubic and LSEVR respectively. Error in magnitude was 3% for both interpolation techniques and 1% for LSEVR showing a clear benefit to using LSEVR for conditions that require multiple clustered vectors to be replaced

Influence of asymmetric valve strategy on large-scale and turbulent in-cylinder flows

Phase-locked particle imaging velocimetry (PIV) measurements are carried out in a direct-injected spark-ignition (DISI) single cylinder optical research engine equipped with fully variable valve timing (FVVT) to assess the impact of asymmetric intake valve lift strategies on the in-cylinder flow. The engine was operated under a range of asymmetric strategies, with one valve following a full lift profile, while the second intake valve is scaled as a factor of the first, expressed as % maximum valve lift (MVL). Proper orthogonal decomposition (POD) combined with a proposed methodology allows instantaneous velocity fields to be decomposed into what are nominally demonstrated as coherent and turbulent constituent velocity fields. Analysis of the coherent fields reveals the behaviour of large scale structures within the flow, subject to cyclic variation. In the case of 40% MVL, an increase in the flow cyclic variability is observed. This is found to be as a result of a switch between a flow dominated by a counter-rotating vortex pair and a single vortex. The impact of MVL on the bulk motion is further evident by an increase in the magnitude of swirl ratio from 0.5 to -6.0 (at 75o CA). Analysis of the turbulent constituent shows how increased valve life asymmetry leads to increased turbulence during the intake stroke by over 250%. Finally, it is shown how the ensemble turbulence statistics may be misleading as stochastic fluctuations were found to be typically 66% of the total TKE calculated from the ensemble statistic in the tested conditions

Distinct phosphorylation clusters determines the signalling outcome of the free fatty acid receptor FFA4/GPR120

It is established that long-chain free fatty acids including ω-3 fatty acids mediate an array of biological responses through members of the free fatty acid receptor family, which includes FFA4. However, the signalling mechanisms and modes of regulation of this receptor class remain unclear. Here we employ mass spectrometry to determine that phosphorylation of mouse (m)FFAR4 occurs at five serine and threonine residues clustered in two separable regions of the C terminal tail, designated cluster 1 (Thr347, Thr349 and Ser350) and cluster 2 (Ser357 and Ser361). Mutation of these phospho-acceptor sites to alanine completely prevented phosphorylation of mFFA4 but did not limit receptor coupling to ERK1/2 activation. Rather an inhibitor of Gq/11 proteins completely prevented receptor signalling to ERK1/2. In contrast, the recruitment of arrestin 3, receptor internalization and activation of Akt were regulated by mFFA4 phosphorylation. The analysis of mFFA4 phosphorylation-dependent signalling was extended further by selective mutations of the phospho-acceptor sites. Mutations within cluster 2 did not affect agonist activation of Akt but instead significantly compromised receptor internalization and arrestin 3 recruitment. Distinctly, mutation of the phospho-acceptor sites within cluster 1 had no effect on receptor internalization and a less extensive effect on arrestin 3 recruitment, but significantly uncoupled the receptor from Akt activation. These unique observations define differential effects on signalling mediated by phosphorylation at distinct locations. This hallmark feature supports the possibility that the signalling outcome of mFFA4 activation can be determined by the pattern of phosphorylation (phosphorylation barcode) at the C-terminus of the receptor

Using negative muons as a probe for depth profiling silver Roman coinage

Debasement of silver Roman coins is a well-known phenomenon and understanding the quality of ancient silver coinages can provide an idea about the underlying fiscal condition of the issuing states. These coins are made from a silver-copper alloy, the surfaces of which were deliberately enhanced at the mints by a process of surface-enrichment to give them the appearance of being made of pure silver. Therefore, any surface analysis would provide a composition of the silver-copper alloy that would not be representative of the original alloy from which the coin blank was made; the result would be too high in silver. However, the bulk of the sample, the interior, should provide a composition that is true to the original alloy. Elemental analysis using negative muons has been used to provide a depth dependent compositional, completely non-destructive analysis of a silver-copper alloy denarius of the empress Julia Domna datable to 211–217 CE. The composition of the coin, beyond the surface enrichment layer, is 51 ± 1.8 % copper and 49 ± 1.9% silver, taken at a muon depth of 402 ± 61 µm. The surface enrichment layer is approximately 190 µm thick

Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture.

Calcium entry through voltage-gated calcium channels has widespread cellular effects upon a host of physiological processes including neuronal excitability, muscle excitation-contraction coupling, and secretion. Using single particle analysis methods, we have determined the first three-dimensional structure, at 23 A resolution, for a member of the low voltage-activated voltage-gated calcium channel family, CaV3.1, a T-type channel. CaV3.1 has dimensions of approximately 115x85x95 A, composed of two distinct segments. The cytoplasmic densities form a vestibule below the transmembrane domain with the C terminus, unambiguously identified by the presence of a His tag being approximately 65 A long and curling around the base of the structure. The cytoplasmic assembly has a large exposed surface area that may serve as a signaling hub with the C terminus acting as a "fishing rod" to bind regulatory proteins. We have also determined a three-dimensional structure, at a resolution of 25 A, for the monomeric form of the cardiac L-type voltage-gated calcium (high voltage-activated) channel with accessory proteins beta and alpha2delta bound to the ion channel polypeptide CaV1.2. Comparison with the skeletal muscle isoform finds a good match particularly with respect to the conformation, size, and shape of the domain identified as that formed by alpha2. Furthermore, modeling of the CaV3.1 structure (analogous to CaV1.2 at these resolutions) into the heteromeric L-type voltage-gated calcium channel complex volume reveals multiple interaction sites for beta-CaV1.2 binding and for the first time identifies the size and organization of the alpha2delta polypeptides

The Stargazin-Related Protein {gamma}7 Interacts with the mRNA-Binding Protein Heterogeneous Nuclear Ribonucleoprotein A2 and Regulates the Stability of Specific mRNAs, Including CaV2.2

The role(s) of the novel stargazin-like {gamma}-subunit proteins remain controversial. We have shown previously that the neuron-specific {gamma}7 suppresses the expression of certain calcium channels, particularly CaV2.2, and is therefore unlikely to operate as a calcium channel subunit. We now show that the effect of {gamma}7 on CaV2.2 expression is via an increase in the degradation rate of CaV2.2 mRNA and hence a reduction of CaV2.2 protein level. Furthermore, exogenous expression of {gamma}7 in PC12 cells also decreased the endogenous CaV2.2 mRNA level. Conversely, knockdown of endogenous {gamma}7 with short-hairpin RNAs produced a reciprocal enhancement of CaV2.2 mRNA stability and an increase in endogenous calcium currents in PC12 cells. Moreover, both endogenous and expressed {gamma}7 are present on intracellular membranes, rather than the plasma membrane. The cytoplasmic C terminus of {gamma}7 is essential for all its effects, and we show that {gamma}7 binds directly via its C terminus to a heterogeneous nuclear ribonucleoprotein (hnRNP A2), which also binds to a motif in CaV2.2 mRNA, and is associated with native CaV2.2 mRNA in PC12 cells. The expression of hnRNP A2 enhances CaV2.2 IBa, and this enhancement is prevented by a concentration of {gamma}7 that alone has no effect on IBa. The effect of {gamma}7 is selective for certain mRNAs because it had no effect on {alpha}2{delta}-2 mRNA stability, but it decreased the mRNA stability for the potassium-chloride cotransporter, KCC1, which contains a similar hnRNP A2 binding motif to that in CaV2.2 mRNA. Our results indicate that {gamma}7 plays a role in stabilizing CaV2.2 mRNA