Performance of the psoroptes ovis - antibody enzyme-linked immunosorbent assay in the face of low-level mite infestation

Psoroptes ovis mites, the causative agent of sheep scab, can severely compromise sheep welfare and production. However, in subclinical infections, mite detection is difficult increasing the risk of spread. A recent serodiagnostic test, based on detecting host antibodies to the P ovis allergen, Pso o 2, has made the detection of subclinical infection possible. The use of this test was demonstrated in subclinical situations, through an opportunistic observational study on an extensive hill farm and a lowland flock with recently introduced, quarantined livestock. Twelve animals were tested from each group. Breeding ewes and lambs on the hill farm had seroprevalences of 16 per cent (12.5–17.8 per cent) and 8.3 per cent (4.8–10.1 per cent), respectively. Quarantined store lambs had a seroprevalence of 16.7 per cent (13.2–18.5 per cent); no evidence of P ovis was found in quarantined replacement ewes. By detecting subclinical infection, this serological test could be a powerful tool in sheep scab control, for quarantine procedures, accreditation programmes, and possibly regional or national eradication protocols

Effect of Air Injection Depth on Big-bubble Formation in Lamellar Keratoplasty: an Ex Vivo Study

This study evaluated the effect of air injection depth in the big-bubble (BB) technique, which is used for corneal tissue preparation in lamellar keratoplasty. The BB technique was performed on ex vivo human corneoscleral buttons using a depth-sensing needle, based on optical coherence tomography (OCT) imaging technology. The needle tip, equipped with a miniaturized OCT depth-sensing probe, was inserted for air injection at a specified depth. Inside the corneal tissue, our needle obtained OCT line profiles, from which residual thickness below the needle tip was measured. Subjects were classified into Groups I, II, III, and IV based on injection depths of 75-80%, 80-85%, 85-90%, and > 90% of the full corneal thickness, respectively. Both Type I and II BBs were produced when the mean residual thicknesses of air injection were 109.7 +/- 38.0 mu m and 52.4 +/- 19.2 mu m, respectively. Type II BB (4/5) was dominant in group IV. Bubble burst occurred in 1/16 cases of type I BB and 3/16 cases of type II BB, respectively. Injection depth was an important factor in determining the types of BBs produced. Deeper air injection could facilitate formation of Type II BBs, with an increased risk of bubble bursts

Effect of Fibrin Glue on the Biomechanical Properties of Human Descemet's Membrane

10.1371/journal.pone.0037456PLoS ONE75

Preliminary observations on the value of using effective anthelmintic drugs to control nematode parasitism in lambs in the face of a high level of infective larval challenge

Gastrointestinal nematode parasites in small ruminants pose a major challenge to global agriculture. In most cases, their control depends on the availability of anthelmintics, which must be used in a manner that achieves a balance between ensuring adequate productivity and minimising the impact of inevitable selection for drug resistance. In this observational field study, the liveweight gains of lambs grazed on putative heavily infective larval contaminated pasture were compared after effective or ineffective anthelmintic drug treatments. Moxidectin, monepantel, and a derquantel–abamectin combination achieved 100% post treatment efficacies in faecal egg count reduction tests, while the efficacy of levamisole against Teladorsagia circumcincta was only 33%. During the 14 days after treatment, lambs treated with moxidectin, monepantel, and a derquantel–abamectin combination, gained about 0.3 kg, 0.5 kg, and 0.7 kg, respectively, more than those treated with levamisole, generally supporting the economic investment in use of effective drugs. These observations serve to highlight the manner in which effective anthelmintic drugs are used most efficiently with the primary objective of minimising subsequent exposure of naïve lambs to high levels of infective larval challenge, as opposed to the more common practice of simply treating animals grazing on heavily contaminated pastures

Hepatogenous photosensitisation in Scottish sheep casued by Dicrocoelium dendriticum

Dicrocoeliosis was identified as the probable predisposing cause of weight loss and hepatogenous photosensitisation affecting half of a group of 14 month-old ewe lambs on a farm on the Inner Hebridean island of Coll. Oral dosing of the ewe lambs with 15 mg/kg albendazole only achieved a 79.2% reduction in Dicrocoelium dendriticum egg count, 21 day post treatment. Thus, this report of dicrocoeliosis differs from the disease that occurs elsewhere, both in the severity and nature of its clinical signs, and in the response of the parasite to anthelmintic drug treatment. These differences could indicate the existence of a genetically divergent D. dendriticum population, which may have evolved within the unique and isolated biotope afforded by the machair on the island of Coll. Better understanding of these factors is a prerequisite for effective and sustainable disease control

Addressing sustainable sheep farming: application of a targeted selective treatment approach for anthelmintic use on a commercial farm

Sustainable control of nematode parasites in small ruminant production is a worldwide ambition. Development of anthelmintic resistance can severely impair small ruminant production. A practical approach to reduce selection pressure for anthelmintic resistance is to treat only a proportion of the flock (Targeted Selective Treatment), leaving a proportion of the nematode population untreated. The aim of this study was to compare the sustainability and efficacy of a performance-based marker, the Happy Factor™, a monitor of nutrient utilisation efficiency, with a routine whole flock anthelmintic treatment. In a commercial flock in the South West of Scotland, 183 Texel cross lambs were split into two matched but co-grazing groups: one group managed as routinely for the farm (RT group) and the other subjected to targeted selective treatment (TST group). All lambs from the RT group were drenched every 6 weeks during the grazing season, while anthelmintic administration in the TST group was restricted to animals that failed to reach pre-determined weight gain targets, based on an estimate of their efficiency of gross energy utilisation. Animal performance and parasitological data were recorded every two weeks. In the 20 week period of the study, anthelmintic treatments were reduced by approximately 50% in the TST group compared to a routine anthelmintic administration that would have been applied, whilst epg counts were always <500 throughout the study in both groups. Finally, there was no discernible difference in the mean bodyweight gain between the two groups

Donor-to-host transmission of infection: contrasting outcomes of lamellar and penetrating keratoplasty

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