Relationship of IFNγ and IL10 SNPs with mycobacterial antigen induced cytokine secretion.

<p>Whole blood from TB not affected (TBNA) Tuberculin skin test positive donors was stimulated with <i>M. tuberculosis</i> culture filtrate (CF) proteins [5 µg/ml] and supernatants tested at 2 days for IL10 secretion and day 5 for IFNγ secretion using ELISA method as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#s4" target="_blank">material and methods</a>. Results are expressed as pg/ml after deducting secretion in un-stimulated whole blood.</p

Demographic characteristics of TB Patients and Controls.

<p><b>Note:</b> Patient stratification is as given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#s4" target="_blank">Materials and Methods</a>. Abbreviations for groups are given in brackets. TBA included 10 previously treated patients.</p

Diagnostic modality used for confirmation of tuberculosis.

<p>Note: Primary diagnostic modality used diagnosis of tuberculosis.</p>*<p>Criteria for disease category given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#s4" target="_blank">material and methods</a>.</p>¶<p>Imaging tests included chest×rays for pulmonary patients, CT scan and or MRI for disseminated disease.</p

Genotype combination in relation to disease severity.

<p>Genotype combination in relation to disease severity.</p

Differences in allele frequencies in healthy controls and tuberculosis patients

<p>Note: Patient stratification is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#s4" target="_blank">material and methods</a>. N for each group is given in brackets. Abbreviations used as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#pone-0004778-t001" target="_blank">Table 1</a>.</p

Genotype frequencies in healthy controls and different clinical forms of tuberculosis (TB).

<p>Note: Patient stratification is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#s4" target="_blank">Materials and Methods</a>. N for each group given in brackets. Abbreviations used as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004778#pone-0004778-t001" target="_blank">Table 1</a>. Number (frequency) of genotypes is indicated. Pearson chi analysis was carried to determine the significance of differences.</p><p>All significant p values are indicated in bold. P approaching significance is given in italics. <i>p</i><0.05 is considered significant.</p

Differential LPS-induced CXCL9 and CCL2 in limited and severe TB.

<p>Whole blood cells were stimulated with LPS at 1 µg/ml and CXCL9 measured in cell supernatants harvested at 2 days post-stimulation. All other parameters as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005158#pone-0005158-g001" target="_blank">Fig. 1</a>. A. CXCL9 and B. CCL2.</p

ESAT6- and CFP10-induced cytokine and chemokine responses.

<p>EC, healthy endemic controls; TB, tuberculosis patients.</p><p>‘δ’ denotes cytokine secretion after background subtraction in each case.</p>*<p>denotes significant difference (p<0.05) as compared with EC.</p><p>All statistical analyses performed using the Mann-Whitney U non-parametric test.</p

Differential ESAT6-induced IFNγ and CXCL9 in pulmonary and extrapulmonary TB.

<p>Whole blood cells were stimulated with ESAT6 at 5 µg/ml, and IFNγ measured in cell supernatants harvested at 5 day post-stimulation, while CXCL9 was measured at 2 days post-stimulation. TB patient groups comprised those with pulmonary TB (PTB, n = 30), less severe EPulTB (LNTB, n = 24) and severe EPulTB (SevTB, n = 22). The box plots represent data from each group after cytokine secretion from unstimulated cells has been subtracted. The whiskers indicate the 25^th and 75^th quartile respectively, while the median line separates the two. ‘*’ denotes significant differences between groups, p<0.05; (*). A. IFNγ, B. CXCL9.</p

Type of fluid therapy adminstered.

<p>N/S  =  Normal saline.</p><p>R/L  =  Ringer's lactate.</p><p>ORS  =  Oral Rehydration Solution.</p><p>D/S  =  Dextrose Saline.</p