192 research outputs found

    Legal certainty of legality principle in the legislation of the continental and anglo-american system

    Get PDF
    In this article, through the comparative legal analysis of domestic, as well as foreign constitutional and criminal law norm content, various approaches to the principle of legality and its main features were examine

    Nonisospectral integrable nonlinear equations with external potentials and their GBDT solutions

    Full text link
    Auxiliary systems for matrix nonisospectral equations, including coupled NLS with external potential and KdV with variable coefficients, were introduced. Explicit solutions of nonisospectral equations were constructed using the GBDT version of the B\"acklund-Darboux transformation

    Methods and techniques for identifying signs of extremism

    Get PDF
    In the present reality, the public distribution of audio, video and printed materials containing signs of extremism causes serious social and state danger. In this regard, it is important to determine a clear algorithm for the activity of an expert in organizing research in order to identify signs of extremism, namely the determination of the most important methods and techniques for working with disputed material

    Explicit solutions of the four-wave mixing model

    Full text link
    The dynamical degenerate four-wave mixing is studied analytically in detail. By removing the unessential freedom, we first characterize this system by a lower-dimensional closed subsystem of a deformed Maxwell-Bloch type, involving only three physical variables: the intensity pattern, the dynamical grating amplitude, the relative net gain. We then classify by the Painleve' test all the cases when singlevalued solutions may exist, according to the two essential parameters of the system: the real relaxation time tau, the complex response constant gamma. In addition to the stationary case, the only two integrable cases occur for a purely nonlocal response (Real(gamma)=0), these are the complex unpumped Maxwell-Bloch system and another one, which is explicitly integrated with elliptic functions. For a generic response (Re(gamma) not=0), we display strong similarities with the cubic complex Ginzburg-Landau equation.Comment: 16 pages, J Phys A Fast track communication, to appear 200

    A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

    Get PDF
    Genetically encoded calcium indicators (GECIs) are mainly represented by two- or one-fluorophore-based sensors. One type of two-fluorophore-based sensor, carrying Opsanus troponin C (TnC) as the Ca2+-binding moiety, has two binding sites for calcium ions, providing a linear response to calcium ions. One-fluorophore-based sensors have four Ca2+-binding sites but are better suited for in vivo experiments. Herein, we describe a novel design for a one-fluorophore-based GECI with two Ca2+-binding sites. The engineered sensor, called NTnC, uses TnC as the Ca2+-binding moiety, inserted in the mNeonGreen fluorescent protein. Monomeric NTnC has higher brightness and pH-stability in vitro compared with the standard GECI GCaMP6s. In addition, NTnC shows an inverted fluorescence response to Ca2+. Using NTnC, we have visualized Ca2+ dynamics during spontaneous activity of neuronal cultures as confirmed by control NTnC and its mutant, in which the affinity to Ca2+ is eliminated. Using whole-cell patch clamp, we have demonstrated that NTnC dynamics in neurons are similar to those of GCaMP6s and allow robust detection of single action potentials. Finally, we have used NTnC to visualize Ca2+ neuronal activity in vivo in the V1 cortical area in awake and freely moving mice using two-photon microscopy or an nVista miniaturized microscope
    corecore