Impact of PRRSV infection and dietary soybean meal on ileal amino acid digestibility and endogenous amino acid losses in growing pigs

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant disease in the swine industry, and increasing soybean meal (SBM) consumption during this disease challenge may improve performance. Our objectives were to determine the impact of SBM level on apparent total tract (ATTD) and ileal (AID) digestibility during PRRSV infection and to determine ileal basal endogenous losses (BEL) during PRRSV infection. Forty PRRSV negative gilts were fitted with a T-cannula in the distal ileum. Treatments were arranged in a 2 × 2 factorial with high and low SBM (HSBM, 29% vs. LSBM, 10%), with and without PRRSV (n = 6/treatment). The remaining pigs (n = 8/challenge status) were fed a N-free diet. Chromic oxide was used as an indigestible marker. On day post inoculation (dpi) 0, at 47.7 ± 0.57 kg BW, 20 pigs were inoculated with live PRRSV; 20 control pigs were sham inoculated. Infection was confirmed by serum PCR. Feces were collected at dpi 5 to 6 and dpi 16 to 17, and ileal digesta collected at dpi 7 to 8 and dpi 18 to 19. Feed, feces, and digesta were analyzed for DM, N, and GE. Digesta and feed were analyzed for AA. Data were analyzed in a 2 × 2 + 2 factorial design to determine main effects of diet and PRRSV and their interaction. Data from N-free fed pigs were analyzed separately to determine BEL and hindgut disappearance due to PRRSV infection. All control pigs remained PRRSV negative. There were no interactions for AID of AA; however, HSBM reduced DM, GE, Lys, and Met AID and increased Arg and Gly AID during both collection periods (P \u3c 0.05). At dpi 7 to 8 only, PRRSV reduced DM and GE AID (P \u3c 0.05). At 7 to 8 dpi, BEL of Arg, Ala, and Pro were reduced (P \u3c 0.05) due to PRRSV by 64%, 39%, and 94%, respectively. At dpi 18 to 19, BEL of Thr tended (P = 0.06) to be increased in PRRSV-infected pigs; however, no other differences were observed. Pigs fed LSBM had increased Lys, Met, Thr, Trp, and Pro standardized ileal digestibility (SID), primarily at 7 to 8 dpi. At 7 to 8 dpi, PRRSV reduced Arg, Gly, and Pro SID (P \u3c 0.01), and SID Pro continued to be reduced by 17% at dpi 18 to 19. Compared with HSBM pigs, LSBM reduced hindgut disappearance of DM and GE at dpi 5 to 8 and dpi 16 to 19, while N disappearance was reduced at dpi 5 to 8. There were no differences between control and PRRSV N-free fed pigs. Altogether, SBM inclusion impacts SID of AA and hindgut disappearance of nutrients, regardless of PRRSV. In contrast, there is minimal impact of PRRSV on BEL, and therefore, SID of most AA are not different

Impact of Brachyspira hyodysenteriae on intestinal amino acid digestibility and endogenous amino acid losses in pigs

Brachyspira hyodysenteriae (Bhyo) induces mucohemorrhagic diarrhea in pigs and is an economically significant disease worldwide. Our objectives were to determine the impact of Bhyo on apparent total tract digestibility (ATTD), ileal digestibility (AID), and ileal basal endogenous losses (BEL) in grower pigs. In addition, we assessed the effect of Bhyo on hindgut disappearance of DM, N, and GE. Thirty-two Bhyo negative gilts (38.6 ± 0.70 kg BW) were fitted with a T-cannula in the distal ileum and individually penned. In replicates 1 and 2, pigs were fed a complete diet (7 Bhyo−, 10 Bhyo+ pigs) or nitrogen-free diet (NFD; 4 Bhyo−, 11 Bhyo+ pigs), respectively. Across multiple rooms, the 21 Bhyo+ pigs (62.6 ± 1.39 kg BW) were inoculated with Bhyo on day post inoculation (dpi) 0, and the 11 Bhyo− pigs were sham inoculated. Feces were collected from 9 to 11 dpi and ileal digesta collected from 12 to 13 dpi. All pigs were euthanized at 14 to 15 dpi and intestinal tract pathology assessed. Within the complete diet and NFD treatments, data were analyzed to determine pathogen effects. All Bhyo− pigs remained Bhyo negative, and 5 Bhyo+ pigs in each replicate were confirmed Bhyo positive within 9 dpi. Infection with Bhyo reduced ATTD of DM, N, and GE and increased AID of Gly (P \u3c 0.05). No other AA AID differences were observed. Only BEL of Pro was reduced (P \u3c 0.05) while Arg, Trp, and Gly tended (P \u3c 0.10) to be reduced in Bhyo+ pigs. When calculated from AID and BEL, Bhyo infection reduced standardized ileal digestibility (SID) of N, Arg, Lys, Ala, Gly, Pro, and Ser (P \u3c 0.05) and tended to reduce Thr SID (P = 0.09). In the hindgut of Bhyo+ pigs, there was generally an appearance of nutrients rather than disappearance. In Bhyo+ pigs fed a complete diet, hindgut appearance of N and GE were increased (P \u3c 0.05) by 58 and nine-fold, respectively, and DM tended to be increased two-fold (P = 0.06). Similarly, in NFD fed pigs, hindgut appearance of N and GE was increased by 172% and 162%, respectively, although high variability led to no significance. Altogether, Bhyo infection decreases ATTD but has minimal impact on AID of AA, when corrected for BEL, SID of N, Arg, Lys and some nonessential AA are specifically reduced. Unexpectedly, BEL of several AA involved in mucin production were unaffected by Bhyo infection. This may suggest an increased need for specific AA and energy during a Bhyo challenge

Impact of PRRSV infection and dietary soybean meal on ileal amino acid digestibility and endogenous amino acid losses in growing pigs

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant disease in the swine industry, and increasing soybean meal (SBM) consumption during this disease challenge may improve performance. Our objectives were to determine the impact of SBM level on apparent total tract (ATTD) and ileal (AID) digestibility during PRRSV infection and to determine ileal basal endogenous losses (BEL) during PRRSV infection. Forty PRRSV negative gilts were fitted with a T-cannula in the distal ileum. Treatments were arranged in a 2 × 2 factorial with high and low SBM (HSBM, 29% vs. LSBM, 10%), with and without PRRSV (n = 6/treatment). The remaining pigs (n = 8/challenge status) were fed a N-free diet. Chromic oxide was used as an indigestible marker. On day post inoculation (dpi) 0, at 47.7 ± 0.57 kg BW, 20 pigs were inoculated with live PRRSV; 20 control pigs were sham inoculated. Infection was confirmed by serum PCR. Feces were collected at dpi 5 to 6 and dpi 16 to 17, and ileal digesta collected at dpi 7 to 8 and dpi 18 to 19. Feed, feces, and digesta were analyzed for DM, N, and GE. Digesta and feed were analyzed for AA. Data were analyzed in a 2 × 2 + 2 factorial design to determine main effects of diet and PRRSV and their interaction. Data from N-free fed pigs were analyzed separately to determine BEL and hindgut disappearance due to PRRSV infection. All control pigs remained PRRSV negative. There were no interactions for AID of AA; however, HSBM reduced DM, GE, Lys, and Met AID and increased Arg and Gly AID during both collection periods (P P P P = 0.06) to be increased in PRRSV-infected pigs; however, no other differences were observed. Pigs fed LSBM had increased Lys, Met, Thr, Trp, and Pro standardized ileal digestibility (SID), primarily at 7 to 8 dpi. At 7 to 8 dpi, PRRSV reduced Arg, Gly, and Pro SID (P This article is published as Schweer, Wesley P., John F. Patience, Eric R. Burrough, Brian J. Kerr, and Nicholas K. Gabler. "Impact of PRRSV infection and dietary soybean meal on ileal amino acid digestibility and endogenous amino acid losses in growing pigs." Journal of animal science 96, no. 5 (2018): 1846-1859. doi:10.1093/jas/sky093.</p

Impact of Brachyspira hyodysenteriae on intestinal amino acid digestibility and endogenous amino acid losses in pigs

Brachyspira hyodysenteriae (Bhyo) induces mucohemorrhagic diarrhea in pigs and is an economically significant disease worldwide. Our objectives were to determine the impact of Bhyo on apparent total tract digestibility (ATTD), ileal digestibility (AID), and ileal basal endogenous losses (BEL) in grower pigs. In addition, we assessed the effect of Bhyo on hindgut disappearance of DM, N, and GE. Thirty-two Bhyo negative gilts (38.6 ± 0.70 kg BW) were fitted with a T-cannula in the distal ileum and individually penned. In replicates 1 and 2, pigs were fed a complete diet (7 Bhyo−, 10 Bhyo+ pigs) or nitrogen-free diet (NFD; 4 Bhyo−, 11 Bhyo+ pigs), respectively. Across multiple rooms, the 21 Bhyo+ pigs (62.6 ± 1.39 kg BW) were inoculated with Bhyo on day post inoculation (dpi) 0, and the 11 Bhyo− pigs were sham inoculated. Feces were collected from 9 to 11 dpi and ileal digesta collected from 12 to 13 dpi. All pigs were euthanized at 14 to 15 dpi and intestinal tract pathology assessed. Within the complete diet and NFD treatments, data were analyzed to determine pathogen effects. All Bhyo− pigs remained Bhyo negative, and 5 Bhyo+ pigs in each replicate were confirmed Bhyo positive within 9 dpi. Infection with Bhyo reduced ATTD of DM, N, and GE and increased AID of Gly (P P P P P = 0.09). In the hindgut of Bhyo+ pigs, there was generally an appearance of nutrients rather than disappearance. In Bhyo+ pigs fed a complete diet, hindgut appearance of N and GE were increased (P P = 0.06). Similarly, in NFD fed pigs, hindgut appearance of N and GE was increased by 172% and 162%, respectively, although high variability led to no significance. Altogether, Bhyo infection decreases ATTD but has minimal impact on AID of AA, when corrected for BEL, SID of N, Arg, Lys and some nonessential AA are specifically reduced. Unexpectedly, BEL of several AA involved in mucin production were unaffected by Bhyo infection. This may suggest an increased need for specific AA and energy during a Bhyo challenge.This article is published as Schweer, Wesley P., Eric R. Burrough, John F. Patience, Brian J. Kerr, and Nicholas K. Gabler. "Impact of Brachyspira hyodysenteriae on intestinal amino acid digestibility and endogenous amino acid losses in pigs." Journal of animal science 97, no. 1 (2019): 257-268. doi:10.1093/jas/sky393.</p

Disseminated aspergillosis in a dog due to Aspergillus alabamensis

Disseminated aspergillosis is uncommon in dogs and often associated with Aspergillus terreus. A case of disseminated disease in an English springer spaniel is reported from which Aspergillus alabamensis was recovered by culture and identified by molecular means suggesting a potential role for this agent as a primary pathogen of dogs.This article is published as Burrough, Eric, Krysta Deitz, Joann Kinyon, Claire Andreasen, Timothy Frana, Deanna Sutton, Elizabeth Thompson, Jianmin Fu, Brian Wickes, and Jesse Hostetter. "Disseminated aspergillosis in a dog due to Aspergillus alabamensis." Medical Mycology Case Reports 1, no. 1 (2012): 1-4. DOI: 10.1016/j.mmcr.2012.02.002. Copyright 2012 International Society for Human and Animal Mycology. Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) Posted with permission

Waste disposal site location, Piedmont Region, Italy, 1:500,000 scale inSpecial purpose mapping for waste disposal sites Report of IAEG Commission 1: Engineering Geological Maps

This report results from work that was first proposed to a meeting of the International Association of Engineering Geology (IAEG), Commission No.1 held in Bratislava (Slovakia) in 1992. Following the publication of the Commission’s report on Engineering Geological Maps (Engineering geological maps (1976) and reports on the description and classification of rock and soil and on recommended symbols for engineering geological mapping (Bull. Int. Assoc. Eng. Geol. 24 (1981) 235; see also 227), a period of consolidation ensued in which the approaches suggested in those reports were implemented. The decision to prepare an additional report reflected the increasing need to apply these approaches to a range of specific activities. The background to the report was provided in a paper by two of the authors presented at the IAEG conference in Athens in 1997 (Proc Int. Symp. Int. Assoc. Eng. Geol. Environ. 2 (1997) 2103)