Spatial Constraint Corrections to the Elasticity of dsDNA Measured with Magnetic Tweezers

In this paper, we have studied, within a discrete WLC model, the spatial constraints in magnetic tweezers used in single molecule experiments. Two elements are involved: first, the fixed plastic slab on which is stuck the initial strand, second, the magnetic bead which pulls (or twists) the attached molecule free end. We have shown that the bead surface can be replaced by its tangent plane at the anchoring point, when it is close to the bead south pole relative to the force. We are led to a model with two parallel repulsive plates: the fixed anchoring plate and a fluctuating plate, simulating the bead, in thermal equilibrium with the system. The bead effect is a slight upper shift of the elongation, about four times smaller than the similar effect induced by the fixed plate. This rather unexpected result, has been qualitatively confirmed within the soluble Gaussian model. A study of the molecule elongation versus the countour length exhibits a significant non-extensive behaviour. The curve for short molecules (with less than 2 kbp) is well fitted by a straight line, with a slope given by the WLC model, but it does not go through the origin. The non-extensive offset gives a 15% upward shift to the elongation of a 2 kbp molecule stretched by a 0.3 pN force.Comment: 28 pages, 6 figures An explanatory figure has been added. The physical interpretation of the results has been made somewhat more transparen

Small business in Ukraine: problems and opportunity of efficient functioning

Статтю присвячено дослідженню проблем малого підприємництва та можливості його ефективного функціонування в сучасних ринкових умовах. Малий бізнес є невід’ємною складовою ринкового господарства. Функціонування в сучасних ринкових умовах надає йому гнучкості, мобілізує фінансові й виробничі ресурси, прискорює темпи науково-технічного прогресу, вирішує проблему зайнятості населення. Тому багатостороння підтримка розвитку малого бізнесу та побудова соціально орієнтованої економіки має стати головним вектором реформ в Україні, що служитиме фактором підвищення рівня життя населення та сприятиме процесам інтеграції національної економіки у світове глобальне господарство.The article is devoted to the problems of small business and the possibility of its effective functioning in the current market conditions. Small business is an integral part of the market economy. Functioning in the current market conditions gives it flexibility, mobilizes financial and industrial resources, boosts scientific and technological progress, solves the problem of unemployment. Therefore, multilateral support of small business development and building a socially oriented economy should become the main vectors of reforms in Ukraine, which will serve as factorsof improving standards of life and facilitating the integration of national economy into the world global economy

Simulation of a semiflexible polymer in a narrow cylindrical pore

The probability that a randomly accelerated particle in two dimensions has not yet left a simply connected domain ${\cal A}$ after a time $t$ decays as $e^{-E_0t}$ for long times. The same quantity $E_0$ also determines the confinement free energy per unit length $\Delta f=k_BT\thinspace E_0$ of a semiflexible polymer in a narrow cylindrical pore with cross section ${\cal A}$. From simulations of a randomly accelerated particle we estimate the universal amplitude of $\Delta f$ for both circular and rectangular cross sections.Comment: 10 pages, 2 eps figure

Casimir Forces between Spherical Particles in a Critical Fluid and Conformal Invariance

Mesoscopic particles immersed in a critical fluid experience long-range Casimir forces due to critical fluctuations. Using field theoretical methods, we investigate the Casimir interaction between two spherical particles and between a single particle and a planar boundary of the fluid. We exploit the conformal symmetry at the critical point to map both cases onto a highly symmetric geometry where the fluid is bounded by two concentric spheres with radii R_- and R_+. In this geometry the singular part of the free energy F only depends upon the ratio R_-/R_+, and the stress tensor, which we use to calculate F, has a particularly simple form. Different boundary conditions (surface universality classes) are considered, which either break or preserve the order-parameter symmetry. We also consider profiles of thermodynamic densities in the presence of two spheres. Explicit results are presented for an ordinary critical point to leading order in epsilon=4-d and, in the case of preserved symmetry, for the Gaussian model in arbitrary spatial dimension d. Fundamental short-distance properties, such as profile behavior near a surface or the behavior if a sphere has a `small' radius, are discussed and verified. The relevance for colloidal solutions is pointed out.Comment: 37 pages, 2 postscript figures, REVTEX 3.0, published in Phys. Rev. B 51, 13717 (1995

Entropic Elasticity of Double-Strand DNA Subject to Simple Spatial Constraints

The aim of the present paper is the study of the entropic elasticity of the dsDNA molecule, having a cristallographic length L of the order of 10 to 30 persistence lengths A, when it is subject to spatial obstructions. We have not tried to obtain the single molecule partition function by solving a Schodringer-like equation. We prefer to stay within a discretized version of the WLC model with an added one-monomer potential, simulating the spatial constraints. We derived directly from the discretized Boltzmann formula the transfer matrix connecting the partition functions relative to adjacent "effective monomers". We have plugged adequate Dirac delta-functions in the functional integral to ensure that the monomer coordinate and the tangent vector are independent variables. The partition function is, then, given by an iterative process which is both numerically efficient and physically transparent. As a test of our discretized approach, we have studied two configurations involving a dsDNA molecule confined between a pair of parallel plates.Comment: The most formal developments of Section I have been moved into an appendix and replaced by a direct derivation of the transfer matrix used in the applications. of Section II. Two paragraphs and two figures have been added to clarify the physical interpretation of the result

Critical behaviour near multiple junctions and dirty surfaces in the two-dimensional Ising model

We consider m two-dimensional semi-infinite planes of Ising spins joined together through surface spins and study the critical behaviour near to the junction. The m=0 limit of the model - according to the replica trick - corresponds to the semi-infinite Ising model in the presence of a random surface field (RSFI). Using conformal mapping, second-order perturbation expansion around the weakly- and strongly-coupled planes limits and differential renormalization group, we show that the surface critical behaviour of the RSFI model is described by Ising critical exponents with logarithmic corrections to scaling, while at multiple junctions (m>2) the transition is first order. There is a spontaneous junction magnetization at the bulk critical point.Comment: Old paper, for archiving. 6 pages, 1 figure, IOP macro, eps

Non-Universal Critical Behaviour of Two-Dimensional Ising Systems

Two conditions are derived for Ising models to show non-universal critical behaviour, namely conditions concerning 1) logarithmic singularity of the specific heat and 2) degeneracy of the ground state. These conditions are satisfied with the eight-vertex model, the Ashkin-Teller model, some Ising models with short- or long-range interactions and even Ising systems without the translational or the rotational invariance.Comment: 17 page

Conformal off-diagonal boundary density profiles on a semi-infinite strip

The off-diagonal profile phi(v) associated with a local operator (order parameter or energy density) close to the boundary of a semi-infinite strip with width L is obtained at criticality using conformal methods. It involves the surface exponent x_phi^s and displays a simple universal behaviour which crosses over from surface finite-size scaling when v/L is held constant to corner finite-size scaling when v/L -> 0.Comment: 5 pages, 1 figure, IOP macros and eps

Shock statistics in higher-dimensional Burgers turbulence

We conjecture the exact shock statistics in the inviscid decaying Burgers equation in D>1 dimensions, with a special class of correlated initial velocities, which reduce to Brownian for D=1. The prediction is based on a field-theory argument, and receives support from our numerical calculations. We find that, along any given direction, shocks sizes and locations are uncorrelated.Comment: 4 pages, 8 figure