Effective transverse momentum in multiple jet production at hadron colliders

We consider the class of inclusive hadron collider processes in which several energetic jets are produced, possibly accompanied by colorless particles [such as Higgs boson(s), vector boson(s) with their leptonic decays, and so forth]. We propose a new variable that smoothly captures the $N+1$ to $N$-jet transition. This variable, that we dub $k_{ness}^{T}$, represents an effective transverse momentum controlling the singularities of the $N+1$-jet cross section when the additional jet is unresolved. The $k_{ness}^{T}$ variable offers novel opportunities to perform higher-order calculations in quantum chromodynamics by using nonlocal subtraction schemes. We study the singular behavior of the $N+1$-jet cross section as $k_{ness}^{T}→0$ and, as a phenomenological application, we use the ensuing results to evaluate next-to-leading-order corrections to $H+jet$ and $Z+2$-jet production at the LHC. We show that $k_{ness}^{T}$ performs extremely well as a resolution variable and appears to be very stable with respect to hadronization and multiple-parton interactions

Exploring slicing variables for jet processes

We consider the class of inclusive hadron collider processes in which one or more energetic jets are produced, possibly accompanied by colourless particles. We provide a general formulation of a slicing scheme for this class of processes, by identifying the various contributions that need to be computed up to next-to-leading order (NLO) in QCD perturbation theory. We focus on two novel observables, the one-jet resolution variable $\Delta E_t$ and the $n$-jet resolution variable $k_{T}^{\mathrm{ness}}$, and explicitly compute all the ingredients needed to carry out NLO computations using these variables. We contrast the behaviour of these variables when the slicing parameter becomes small. In the case of $k_{T}^{\mathrm{ness}}$ we also present results for the hadroproduction of multiple jets.Comment: 47 pages, 3 figure

Mixed QCD-electroweak corrections to pp→lνl+X at the LHC

We consider the hadroproduction of a massive charged lepton plus the corresponding neutrino through the Drell-Yan mechanism. We present a new computation of the mixed QCD-electroweak (EW) corrections to this process. The cancellation of soft and collinear singularities is achieved by using a formulation of the qT subtraction formalism derived from the next-to-next-to-leading-order QCD calculation for heavy-quark production. For the first time, all the real and virtual contributions due to initial- and final-state radiation are consistently included without any approximation, except for the finite part of the two-loop virtual correction, which is computed in the pole approximation and suitably improved through a reweighting procedure. We demonstrate that our calculation is reliable in both on-shell and off-shell regions, thereby providing the first prediction of the mixed QCD-EW corrections in the entire region of the lepton transverse momentum. The computed corrections are in qualitative agreement with what we obtain in a factorized approach of QCD and EW corrections. At large values of the lepton pT, the mixed QCD-EW corrections are negative and increase in size, to about −20% with respect to the next-to-leading-order QCD result at pT=500  GeV

Associated production of a WW boson and massive bottom quarks at next-to-next-to-leading order in QCD

We present the first calculation for the hadroproduction of a $W$ boson in association with a massive bottom ($b$) quark-antiquark pair at next-to-next-to-leading order (NNLO) in QCD perturbation theory. We exploit the hierarchy between the $b$ quark mass and the characteristic energy scale of the process to obtain a reliable analytic expression for the two-loop virtual amplitude with three massive legs, starting from the corresponding result available for massless bottom quarks. The use of massive $b$ quarks avoids the ambiguities associated with the correct flavour assignment in massless calculations, paving the way to a more realistic comparison with experimental data. We present phenomenological results considering proton-proton collisions at centre-of-mass energy $\sqrt{s}=13.6$ TeV for inclusive $Wb \bar b$ production and within a fiducial region relevant for the associated production of a $W$ boson and a Higgs boson decaying into a $b \bar b$ pair, for which $Wb \bar b$ production represents one of the most relevant backgrounds. We find that the NNLO corrections are substantial and that their inclusion is mandatory to obtain reliable predictions.Comment: 8 pages, 2 figures, 2 table

Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme

BACKGROUND: Fungal laccases are useful enzymes for industrial applications; they exhibit broad substrate specificity and thus are able to oxidize a variety of xenobiotic compounds including chlorinated phenolics, synthetic dyes, pesticides and polycyclic aromatic hydrocarbons. Unfortunately, the biotechnological exploitation of laccases can be hampered by the difficulties concerning the enzyme production by the native hosts. RESULTS: In order to obtain a simple and efficient source of laccase, the lcc1 cDNA isolated from the white-rot fungus Trametes trogii has been successfully expressed in the methylotrophic yeast Pichia pastoris under the control of the methanol induced alcohol oxidase promoter P(AOX1). The recombinant Lcc1 was produced as a secreted protein with the native N-terminal prepropeptide for signal trafficking, and thus easily recovered from the culture medium. At the 1-liter scale, as calculated on the basis of the specific activity, the recombinant protein was produced at a yield of 17 mg/l. The highest production level obtained in fed-batch culture was 2520 U/l, corresponding to a specific productivity of 31.5 U/g biomass. The purified recombinant laccase exhibited a behaviour similar to the main laccase produced by T. trogii. Lcc1 showed high activity in the presence of organic solvents and a high decolourization capacity towards azo, triarylmethane, indigo carmine and anthraquinonic dyes, that could be significantly enhanced in the presence of the redox mediators 1-hydroxybenzotriazole and violuric acid. CONCLUSION: Heterologous expression of T. trogii laccase lcc1 in the methylotrophic yeast P. pastoris was successfully achieved. The biochemical and kinetic characterization of the recombinant protein suggests potential technological applications for this enzyme

Associated production of a W boson with a top-antitop quark pair: next-to-next-to-leading order QCD predictions for the LHC

The production of a top-antitop quark pair in association with a W boson (ttW) is one of the heaviest signatures currently probed at the Large Hadron Collider (LHC). Since the first observation reported in 2015 the corresponding rates have been found to be consistently higher than the Standard Model predictions, which are based on next-to-leading order (NLO) calculations in the QCD and electroweak (EW) interactions. We present the first next-to-next-to-leading order (NNLO) QCD computation of ttW production at hadron colliders. The calculation is exact, except for the finite part of the two-loop virtual corrections, which is computed using two different approaches that lead to consistent results within their uncertainties. We combine the newly computed NNLO QCD corrections with the complete NLO QCD+EW results, thus obtaining the most advanced perturbative prediction available to date for the ttW inclusive cross section. The tension with the latest ATLAS and CMS results remains at the $1\sigma-2\sigma$ level.Comment: 7 pages, 3 figures, 1 tabl

Erythropoietin as a Neuroprotective Drug for Newborn Infants: Ten Years after the First Use

Protective strategies against perinatal brain injury represent a major challenge for modern neonatology. Erythropoietin (Epo) enhances endogenous mechanisms of repair and angiogenesis. In order to analyse the newest evidence on the role of Epo in prematurity, hypoxic ischemic encephalopathy (HIE) and perinatal stroke, a critical review using 2020 PRISMA statement guidelines was conducted. This review uncovered 26 clinical trials examining the use of Epo for prematurity and brain injury-related outcomes. The effects of Epo on prematurity were analysed in 16 clinical trials. Erythropoietin was provided until 32-35 weeks of corrected postnatal age with a dosage between 500-3000 UI/kg/dose. Eight trials reported the Epo effects on HIE term newborn infants: Erythropoietin was administered in the first weeks of life, at different multiple doses between 250-2500 UI/kg/dose, as either an adjuvant therapy with hypothermia or a substitute for hypothermia. Two trials investigated Epo effects in perinatal stroke. Erythropoietin was administered at a dose of 1000 IU/kg for three days. No beneficial effect in improving morbidity was observed after Epo administration in perinatal stroke. A positive effect on neurodevelopmental outcome seems to occur when Epo is used as an adjuvant therapy with hypothermia in the HIE newborns. Administration of Epo in preterm infants still presents inconsistencies with regard to neurodevelopmental outcome. Clinical trials show significant differences mainly in target population and intervention scheme. The identification of specific markers and their temporal expression at different time of recovery after hypoxia-ischemia in neonates might be implemented to optimize the therapeutic scheme after hypoxic-ischemic injury in the developing brain. Additional studies on tailored regimes, accounting for the risk stratification of brain damage in newborns, are required

Sudden Infant Death Syndrome: Beyond Risk Factors

Sudden infant death syndrome (SIDS) is defined as "the sudden death of an infant under 1 year of age which remains unexplained after thorough investigation including a complete autopsy, death scene investigation, and detailed clinical and pathological review". A significant decrease of SIDS deaths occurred in the last decades in most countries after the beginning of national campaigns, mainly as a consequence of the implementation of risk reduction action mostly concentrating on the improvement of sleep conditions. Nevertheless, infant mortality from SIDS still remains unacceptably high. There is an urgent need to get insight into previously unexplored aspects of the brain system with a special focus on high-risk groups. SIDS pathogenesis is associated with a multifactorial condition that comprehends genetic, environmental and sociocultural factors. Effective prevention of SIDS requires multiple interventions from different fields. Developing brain susceptibility, intrinsic vulnerability and early identification of infants with high risk of SIDS represents a challenge. Progress in SIDS research appears to be fundamental to the ultimate aim of eradicating SIDS deaths. A complex model that combines different risk factor data from biomarkers and omic analysis may represent a tool to identify a SIDS risk profile in newborn settings. If high risk is detected, the infant may be referred for further investigations and follow ups. This review aims to illustrate the most recent discoveries from different fields, analyzing the neuroanatomical, genetic, metabolic, proteomic, environmental and sociocultural aspects related to SIDS

Immunoglobulin T from sea bass (Dicentrarchus labrax L.): Molecular characterization, tissue localization and expression after nodavirus infection

Background Immunoglobulins (Igs) are fundamental components of the adaptive immune system of vertebrates, with the IgT/IgZ isotype specific of Teleosts. In this paper we describe the identification of an IgT heavy chain from the European sea bass (Dicentrarchus labrax L.), its molecular characterization and tissue mRNA localization by in situ hybridization. Results Sea bass IgT consists of 552 aa (Accession Number KM410929) and it contains a putative 19 amino acids long signal peptide and one potential N-glycosylation site. The C-region consists of four CH domains; each contains the cysteine and tryptophan residues required for their correct folding. Based on the recent sequencing of sea bass genome, we have identified five different genomic contigs bearing exons unequivocally pertaining to IgT (CH2, CH3 and CH4), but none corresponded to a complete IgH locus as IgT sequences were found in the highly fragmented assembled genomic regions which could not be assigned to any major scaffold. The 3D structure of sea bass IgT has been modelled using the crystal structure of a mouse Ig gamma as a template, thus showing that the amino acid sequence is suitable for the expected topology referred to an immunoglobulin-like architecture. The basal expression of sea bass IgT and IgM in different organs has been analysed: gut and gills, important mucosal organs, showed high IgT transcripts levels and this was the first indication of the possible involvement of sea bass IgT in mucosal immune responses. Moreover, sea bass IgT expression increased in gills and spleen after infection with nodavirus, highlighting the importance of IgT in sea bass immune responses. In situ hybridization confirmed the presence of IgT transcripts in the gut and it revealed a differential expression along the intestinal tract, with a major expression in the posterior intestine, suggesting the hindgut as a site for the recruitment of IgT+ cells in this species. IgT transcripts were also found in gill filaments and parallel lamellae and, for the first time, we identified scattered IgT positive cells in the liver, with a strong signal in the hepatic parenchyma. Conclusions In conclusion, we performed a full molecular characterization of IgT in sea bass that points out its possible involvement in mucosal immune responses of this species