Ganetespib inhibits proliferation of primary human pheochromocytoma cells.

<p>Bar graphs represent cell counts of tyrosine hydroxylase (TH)-positive first passage primary human pheochromocytoma cells treated with ganetespib. Control indicates untreated cells; vehicle indicates DMSO treatment; the numbers under each bar indicate the ganetespib concentration (micromolar).</p

Ganetespib inhibits tumor metastasis in a spontaneous metastatic pheochromocytoma model.

<p>A) Efficacy of ganetespib in an MTT-luc subcutaneous model of pheochromocytoma. Quantification of tumor growth was determined by luciferase measurement in an IVIS instrument. Data are plotted as mean photon counts (total flux) from regions of interest (ROI). Error bars represent standard deviations. B) Primary tumors excised from the two groups were compared. C and D) <i>Ex vivo</i> bioluminescence signals from lung and liver were compared between the two groups and graphed as total flux as a method of quantification of metastatic burden at the end of the experiment (*p<0.05).</p

17-AAG and ganetespib inhibit cellular proliferation in pheochromocytoma cell lines. A and B) MPC cells, C and D) MTT cells, and E and F) PC12 cells were treated with different concentrations of 17-AAG or ganetespib (from 1 nM to 1 µM) and incubated for 48 hours.

<p>The graph represents the dose-response of log 10 concentrations of 17-AAG or ganetespib versus signal intensity in a thiazolyl blue formazan (MTT)-based assay.</p

Incubation with 17-AAG or ganetespib inhibits cellular migration in pheochromocytoma MTT cells.

<p>Inhibition of migration by 17-AAG-treated (A) or ganetespib -treated (B) MTT cells in a Transwell assay in the presence of serum. Mean number of migrated cells per microscopic field (at 10× magnification) was determined from triplicate wells and is represented in this graph: 17-AAG (C) and ganetespib (D); error bars represent the standard deviation of triplicate determinations.</p

Combination of SAHA and epirubicin in MTT cells.

<p>Combination of SAHA and epirubicin in MTT cells.</p