Gas chromatographic mass spectrometric detection of dihydroxy fatty acids preserved in the 'bound' phase of organic residues of archaeological pottery vessels.

A methodology is presented for the determination of dihydroxy fatty acids preserved in the ‘bound’ phase of organic residues preserved in archaeological potsherds. The method comprises saponification, esterification, silica gel column chromatographic fractionation, and analysis by gas chromatography/mass spectrometry. The electron ionisation mass spectra of the trimethylsilyl ether methyl ester derivatives are characterised by fragment ions arising from cleavage of the bond between the two vicinal trimethylsiloxy groups. Other significant fragment ions are [M–15]+., [M–31]+., m/z 147 and ions characteristic of vicinal disubstituted (trimethylsiloxy) TMSO? groups (?7,8, ?9,10,?11,12 and ?13,14: m/z 304, 332, 360 and 388, respectively). The dihydroxy fatty acids identified in archaeological extracts exhibited carbon numbers ranging from C16 to C22 and concentrations varying from 0.05 to 14.05 ?g g?1. The wide range of dihydroxy fatty acids observed indicates that this approach may be applied confidently in screening archaeological potsherds for the degradation products of monounsaturated fatty acids derived from commodities processed in archaeological pottery vessels

Novel Approach to Leading-Edge Vortex Suppression

A novel approach to reduce the peak lift and pitching moment on a plunging airfoil is investigated through force, moment, and velocity measurements. This approach, unlike previous investigations of delayed flow separation and leading-edge vortex suppression, uses forced separation through deployment of a minitab near the leading edge. The device can be activated for short time intervals during a gust encounter or unsteady maneuver at the expense of short-duration drag increase. Depending on the frequency and the amplitude of the wing motion and the mean angle of attack, roll-up of vorticity and the formation of a vortex can be delayed or even prevented. This change in the vortex dynamics provides effective lift and moment alleviation for post-stall angles of attack and for low reduced frequencies. In contrast, at low angles of attack, the separated shear layer may roll up for the manipulated flow, resulting in vortex shedding, and lift and nosedown pitching moment increase. These two distinct flow regimes cause decreased or increased lift force, with the most effective frequencies scaling with the reduced frequency. In contrast, the borderline between the two regions scales with the Strouhal number based on amplitude and, in particular, with the minimum effective angle of attack during the cycle. The transient response was studied by investigating impulsively started plunging oscillations. During the first cycle, lift reduction is achieved for all frequencies within the range tested

A Smirnov-Bickel-Rosenblatt theorem for compactly-supported wavelets

In nonparametric statistical problems, we wish to find an estimator of an unknown function f. We can split its error into bias and variance terms; Smirnov, Bickel and Rosenblatt have shown that, for a histogram or kernel estimate, the supremum norm of the variance term is asymptotically distributed as a Gumbel random variable. In the following, we prove a version of this result for estimators using compactly-supported wavelets, a popular tool in nonparametric statistics. Our result relies on an assumption on the nature of the wavelet, which must be verified by provably-good numerical approximations. We verify our assumption for Daubechies wavelets and symlets, with N = 6, ..., 20 vanishing moments; larger values of N, and other wavelet bases, are easily checked, and we conjecture that our assumption holds also in those cases

Three-dimensionality of leading-edge vortices on high aspect ratio plunging wings

To investigate the effect of sweep on the leading-edge vortex of high aspect ratio plunging wings, this article presents force, bending moment, and volumetric velocity measurements for high aspect ratio AR = 10 wings with sweep angles of 0° and 40°. The effect of the sweep angle on the bending moment is the largest at the minimum effective angle of attack. This is because as the leading-edge vortex sheds it moves inboard on the unswept wing while moving outboard on the swept wing. Where the leg of the leading-edge vortex connects with the wing there is significant three-dimensional flow. The axial velocity along the vortex filament, which may be towards to the wing tip or the wing root (reversed flow), exhibits increasing magnitude as the effective angle of attack decreases and the vortex filament deforms. Reversed axial flow along the vortex filament has the largest magnitudes for the unswept wing. In the vortex core, jetlike, wakelike, and uniform axial velocity profiles were observed. Unlike the classical vortex breakdown, the transition from the jetlike to the wakelike axial flow does not appear to be abrupt. The measurements also revealed evidence of spanwise instabilities in the leading-edge vortex filament

Tibial osteotomy as a mechanical model of primary osteoarthritis in rats

This study has presented the first purely biomechanical surgical model of osteoarthritis (OA) in rats, which could be more representative of the human primary disease than intra-articular techniques published previously. A surgical tibial osteotomy (TO) was used to induce degenerative cartilage changes in the medial knee of Sprague-Dawley rats. The presence of osteoarthritic changes in the medial knee compartment of the operated animals was evaluated histologically and through analysis of serum carboxy-terminal telepeptides of type II collagen (CTX-II). In-vivo biomechanical analyses were carried out using a musculoskeletal model of the rat hindlimb to evaluate the loading conditions in the knee pre and post-surgically. Qualitative and quantitative medial cartilage degeneration consistent with OA was found in the knees of the operated animals alongside elevated CTX-II levels and increased tibial compressive loading. The potential avoidance of joint inflammation post-surgically, the maintenance of internal joint biomechanics and the ability to quantify the alterations in joint loading should make this model of OA a better candidate for modeling primary forms of the disease in humans

Developing a training course to teach research skills to people with learning disabilities: “It gives us a voice. We CAN be researchers!”.

Background Within learning disability research, it is important to involve people with learning disabilities at all stages, but there are limited opportunities for them to learn about the research process or to gain research skills. Method An eight-session research training course for people with learning disabilities was developed and piloted at a university in London. The focus was on understanding the research process and gaining practical skills in collecting, analysing and presenting research data. Training methods were experimental, with an emphasis on learning by experience in a “fun” way. Results Ten people with learning disabilities completed the course, showing great enthusiasm and commitment. During the final sessions, students developed and conducted their own research projects, choosing “Employment” as their research topic. The training methods were well received. Benefits included an increase in confidence and new work opportunities for several of the students. This paper was co-authored by the tutors and most of the course graduates. Conclusion It is possible for people with learning disabilities to become skilled researchers, but in order to do so, it is important that they have adequate training opportunities. Funding should be made available for more such courses

Mechanisms of root reinforcement in soils:An experimental methodology using four-dimensional X-ray computed tomography and digital volume correlation

Vegetation on railway or highway slopes can improve slope stability through the generation of soil pore water suctions by plant transpiration and mechanical soil reinforcement by the roots. To incorporate the enhanced shearing resistance and stiffness of root-reinforced soils in stability calculations, it is necessary to understand and quantify its effectiveness. This requires integrated and sophisticated experimental and multiscale modelling approaches to develop an understanding of the processes at different length scales, from individual root-soil interaction through to full soil-profile or slope scale. One of the challenges with multiscale models is ensuring that they sufficiently closely represent real behaviour. This requires calibration against detailed high-quality and data-rich experiments. This study presents a novel experimental methodology, which combines in situ direct shear loading of a willow root reinforced soil with X-ray computed tomography to capture the 3D chronology of soil and root deformation within the shear zone. Digital volume correlation (DVC) analysis was applied to the computed tomography (CT) dataset to obtain full-field 3D displacement and strain information. This paper demonstrates the feasibility and discusses the challenges associated with DVC experiments on root-reinforced soils

Radiocarbon dating wooden carvings and skeletal remains from Pitch Lake, Trinidad

Since the mid 19th century, rare prehistoric wooden carvings and human skeletal remains have been dredged from Pitch Lake, Trinidad, during commercial asphalt mining. Establishing a chronology for these objects is challenging, due to both a lack of stratigraphic and contextual information and the necessity to completely remove any pitch to ensure accurate radiocarbon dates. A range of solvent extraction protocols was tested to identify the most suitable one for pretreating the Pitch Lake artefacts, and then applied to ten wooden objects and a human cranium recovered from the lake. Several of these objects yielded earlier dates than expected, raising concerns that pitch had remained after pretreatment and had affected the dates. Pyrolysis-GC/MS and optical microscopy techniques were applied to material from the human cranium, a weaving tool, and a small bowl. These techniques, as well as routinely applied laboratory quality assurance procedures, indicated that there was no residual pitch within the cranium or the weaving tool after pretreatment, giving confidence to the dates. However, the small bowl was observed to still be contaminated with pitch after extensive pretreatment, indicating that the date is too old and can only be considered as a terminus post quem