19 research outputs found
Zero sound in a quantum gas of spin-3/2 atoms with multipole exchange interaction
In the context of quantum gases, we obtain a many-body Hamiltonian for
spin-3/2 atoms with general multipole (spin, quadrupole, and octupole) exchange
interaction by employing the apparatus of irreducible spherical tensor
operators. This Hamiltonian implies the finite-range interaction, whereas, for
zero-range (contact) potentials parameterized by the -wave scattering
length, the multipole exchange interaction becomes irrelevant. Following the
reduced description method for quantum systems, we derive the quantum kinetic
equation for spin-3/2 atoms in a magnetic field and apply it to examine the
high-frequency oscillations known as zero sound.Comment: 21 pages, 2 figure
Broken-axisymmetry state and magnetic state diagram of spin-1 condensate through the prism of quadrupole degrees of freedom
We theoretically study a weakly interacting gas of spin-1 atoms with
Bose-Einstein condensate in external magnetic field within the Bogoliubov
approach. To this end, in contrast to previous studies, we employ the general
Hamiltonian, which includes both spin and quadrupole exchange interactions as
well as the couplings of the spin and quadrupole moment with the external
magnetic field (the linear and quadratic Zeeman terms). The latter is
responsible for the emergence of the broken-axisymmetry state. We also
re-examine ferromagnetic, quadrupolar, and paramagnetic states employing the
proposed Hamiltonian. For all magnetic states, we find the relevant
thermodynamic characteristics such as magnetization, quadrupole moment,
thermodynamic potential, as well as excitation energies for broken-axisymmetry
state. We show that the broken-axisymmetry state can be prepared at three
different regimes of applied magnetic field. We also present the magnetic state
diagrams for each regime of realizing the broken-axisymmetry state.Comment: 14 pages, 2 figures, 4 table
Using an Inducible Promoter of a Gene Encoding <i>Penicillium verruculosum</i> Glucoamylase for Production of Enzyme Preparations with Enhanced Cellulase Performance
<div><p>Background</p><p><i>Penicillium verruculosum</i> is an efficient producer of highly active cellulase multienzyme system. One of the approaches for enhancing cellulase performance in hydrolysis of cellulosic substrates is to enrich the reaction system with β -glucosidase and/or accessory enzymes, such as lytic polysaccharide monooxygenases (LPMO) displaying a synergism with cellulases.</p><p>Results</p><p>Genes <i>bglI</i>, encoding β-glucosidase from <i>Aspergillus niger</i> (AnBGL), and <i>eglIV</i>, encoding LPMO (formerly endoglucanase IV) from <i>Trichoderma reesei</i> (TrLPMO), were cloned and expressed by <i>P</i>. <i>verruculosum</i> B1-537 strain under the control of the inducible <i>gla1</i> gene promoter. Content of the heterologous AnBGL in the secreted multienzyme cocktails (hBGL1, hBGL2 and hBGL3) varied from 4 to 10% of the total protein, while the content of TrLPMO in the hLPMO sample was ~3%. The glucose yields in 48-h hydrolysis of Avicel and milled aspen wood by the hBGL1, hBGL2 and hBGL3 preparations increased by up to 99 and 80%, respectively, relative to control enzyme preparations without the heterologous AnBGL (at protein loading 5 mg/g substrate for all enzyme samples). The heterologous TrLPMO in the hLPMO preparation boosted the conversion of the lignocellulosic substrate by 10–43%; however, in hydrolysis of Avicel the hLPMO sample was less effective than the control preparations. The highest product yield in hydrolysis of aspen wood was obtained when the hBGL2 and hLPMO preparations were used at the ratio 1:1.</p><p>Conclusions</p><p>The enzyme preparations produced by recombinant <i>P</i>. <i>verruculosum</i> strains, expressing the heterologous AnBGL or TrLPMO under the control of the <i>gla1</i> gene promoter in a starch-containing medium, proved to be more effective in hydrolysis of a lignocellulosic substrate than control enzyme preparations without the heterologous enzymes. The enzyme composition containing both AnBGL and TrLPMO demonstrated the highest performance in lignocellulose hydrolysis, providing a background for developing a fungal strain capable to express both heterologous enzymes simultaneously.</p></div
Progress kinetics of Avicel hydrolysis by different <i>P. verruculosum</i> preparations.
<p>Conditions: substrate concentration 100 mg/mL; protein loading 5 mg/g substrate; CDH loading (when applied) 0.1 mg/g substrate; 50°C; pH 5.0.</p
The scheme of cloning pGA-BGL and pGA-EGIV plasmids.
<p>The scheme of cloning pGA-BGL and pGA-EGIV plasmids.</p
Progress kinetics of hydrolysis of pretreated aspen wood by different <i>P. verruculosum</i> preparations.
<p>Conditions: substrate concentration 100 mg/mL; protein loading 5 mg/g substrate; 50°C; pH 5.0.</p
SDS-PAGE of <i>P. verruculosum</i> preparations.
<p><i>M</i>, molecular markers (in kDa); <i>1</i>, hBGL1; <i>2</i>, hBGL2; <i>3</i>, hBGL3; <i>4</i>, PvC1; <i>5</i>, PvC2; <i>6</i>, hLPMO.</p