Subnanomolar detection of tuberculosis biomarker MPT64 in sandwich sers immunoassay on novel low -cost substrate

The use of SERS for detection, analysis and imaging has attracted great interest in the past decade owing to its high sensitivity and molecular fingerprint specificity. There is an increasing interest in scientific community to SERS as it becomes a versatile method for early medical diagnostics and reliable detection of major health threats to humans (e.g. cancer, tuberculosis, etc) and animals [1]. The key component of our SERS-based immunoassay include:1) a capture substrate to specifically adsorb antigens from solution; (2) Extrinsic Raman Labels (ERLs): surface functionalized gold nanoparticles (AuNPs) to bind to captured antigens selectively and generate intense SERS signals, which contain both capture antigen and SERS active readout molecule (4-nitrobenzenethiol (4-NBT))

Subnanomolar detection of tuberculosis biomarker MPT64 in sandwich sers immunoassay on novel low -cost substrate

The use of SERS for detection, analysis and imaging has attracted great interest in the past decade owing to its high sensitivity and molecular fingerprint specificity. There is an increasing interest in scientific community to SERS as it becomes a versatile method for early medical diagnostics and reliable detection of major health threats to humans (e.g. cancer, tuberculosis, etc) and animals [1]. The key component of our SERS-based immunoassay include:1) a capture substrate to specifically adsorb antigens from solution; (2) Extrinsic Raman Labels (ERLs): surface functionalized gold nanoparticles (AuNPs) to bind to captured antigens selectively and generate intense SERS signals, which contain both capture antigen and SERS active readout molecule (4-nitrobenzenethiol (4-NBT))

High sensitivity detection of NBT on novel SERS substrate

Surface-enhanced Raman scattering (SERS) is rapidly evolving as a useful analytical tool in chemical and biological analysis. The most critical aspect of SERS is the research of efficient SERSactive substrates, such as nanostructured surface or nanoparticles of noble metals with suitable physical parameters such as their material, size, shape, and spacing [1]. Generally, Ag and Au substrates are regarded as one of the best candidates for SERS. Nevertheless, fabrication of SERS substrates with both high sensitivity and high reproducibility remains difficult, and it is costly for routine SERS detection. We report the novel combined substrate for highly sensitive Surface Enhanced Raman spectroscopy (SERS) are capable of detecting 4-nitrobenzenethiol (NBT), as a model Raman active molecule, at very low concentration

High sensitivity detection of NBT on novel SERS substrate

Surface-enhanced Raman scattering (SERS) is rapidly evolving as a useful analytical tool in chemical and biological analysis. The most critical aspect of SERS is the research of efficient SERSactive substrates, such as nanostructured surface or nanoparticles of noble metals with suitable physical parameters such as their material, size, shape, and spacing [1]. Generally, Ag and Au substrates are regarded as one of the best candidates for SERS. Nevertheless, fabrication of SERS substrates with both high sensitivity and high reproducibility remains difficult, and it is costly for routine SERS detection. We report the novel combined substrate for highly sensitive Surface Enhanced Raman spectroscopy (SERS) are capable of detecting 4-nitrobenzenethiol (NBT), as a model Raman active molecule, at very low concentration

Quantifying impact of the low-cost SERS substrate on SERS signal

Surface-Enhanced Raman Spectroscopy (SERS) allows structural detection of molecules absorbed on rough noble metal surfaces by amplifying a normally weak Raman signal. It can be applied in forensic science, biosensing, food safety, and medical diagnostics [1]. Although costly gold and silver films are used as SERS substrates, AuNPs (gold nanoparticles) on aluminum foil can become a low-cost alternative

Quantifying impact of the low-cost SERS substrate on SERS signal

Surface-Enhanced Raman Spectroscopy (SERS) allows structural detection of molecules absorbed on rough noble metal surfaces by amplifying a normally weak Raman signal. It can be applied in forensic science, biosensing, food safety, and medical diagnostics [1]. Although costly gold and silver films are used as SERS substrates, AuNPs (gold nanoparticles) on aluminum foil can become a low-cost alternative

Profile Prediction and Fabrication of Wet-Etched Gold Nanostructures for Localized Surface Plasmon Resonance

Dispersed nanosphere lithography can be employed to fabricate gold nanostructures for localized surface plasmon resonance, in which the gold film evaporated on the nanospheres is anisotropically dry etched to obtain gold nanostructures. This paper reports that by wet etching of the gold film, various kinds of gold nanostructures can be fabricated in a cost-effective way. The shape of the nanostructures is predicted by profile simulation, and the localized surface plasmon resonance spectrum is observed to be shifting its extinction peak with the etching time

Control of data centers’ load and resources for virtual hosting

Розглянуто проблему управління навантаженням і ресурсами центрів оброблення даних. Змодельовано і досліджено систему розподілу ресурсів і динамічного управління навантаженням серверів центру оброблення даних при віртуальному хостингу. Розглянуто існуючі рішення. На основі аналізу виявлених чинників впливу запропоновано загальний підхід до вирішення проблеми. У вигляді задач змішаного програмування з неперервними і булевими змінними сформульовано моделі розподілу екземплярів застосувань між фізичними серверами за різними критеріями ефективності з урахуванням ресурсних та інших обмежень. Для розв’язання задач запропоновано варіанти генетичного та евристичного алгоритмів, а також комплексний алгоритм, побудований на вирішенні задачі лінійного програмування із наступним використанням направленого перебору для визначення значень булевих змінних. Описано структуру й функціонування системи управління інфраструктурою інформаційних технологій, у якій реалізовано запропоновані в статті моделі й алгоритми вирішення зазначеної проблеми.Data centers’ load and resources control problem is considered. The system of data center’s servers’ resources distribution and dynamical load control for virtual hosting is modeling and researching. The available solutions review is fulfilled. On base of influence factors analysis the general approach to solve this problem is proposed. The models of application instances placement between servers under different criteria providing resources and other limitations in form of mixed programming tasks with continuous and Boolean variables are elaborated. The variants of genetic and heuristic algorithms, and also complex algorithm, based on linear programming tasks solution with next step realization of directed search schema to find values for Boolean variables for this problem solving are proposed. The structure and functioning description of information technologies infrastructure control system, in which proposed models and algorithms were realized, are described